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AB249316

Anti-AZIN2 antibody [EPR7124(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal AZIN2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

ADC, KIAA1945, ODCP, AZIN2, Antizyme inhibitor 2, AzI2, Arginine decarboxylase, Ornithine decarboxylase-like protein, ornithine decarboxylase paralog, ARGDC, ODC-like protein, ODC-p

2 Images
Flow Cytometry (Intracellular) - Anti-AZIN2 antibody [EPR7124(2)] - BSA and Azide free (AB249316)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-AZIN2 antibody [EPR7124(2)] - BSA and Azide free (AB249316)

This data was developed using ab157214, the same antibody clone in a different buffer formulation.

Overlay histogram showing SH-SY5Y cells stained with ab157214 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab157214, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Western blot - Anti-AZIN2 antibody [EPR7124(2)] - BSA and Azide free (AB249316)
  • WB

Unknown

Western blot - Anti-AZIN2 antibody [EPR7124(2)] - BSA and Azide free (AB249316)

This data was developed using ab157214, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AZIN2 antibody [EPR7124(2)] (<a href='/en-us/products/primary-antibodies/azin2-antibody-epr71242-ab157214'>ab157214</a>) at 1/1000 dilution

All lanes:

Human cerebellum lysate at 10 µg

Predicted band size: 50 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7124(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249316 is the carrier-free version of ab157214.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target AZIN2 also known as Antizyme Inhibitor 2 is a protein that modulates polyamine levels within cells by inhibiting antizyme activity. It weighs approximately 47 kDa. AZIN2 expresses in various tissues including the brain and adrenal glands where its role is closely associated with polyamine biosynthesis. The protein interacts with antizymes to regulate their function which in turn controls the degradation of ornithine decarboxylase and the uptake of polyamines.
Biological function summary

AZIN2 functions as a modulator of the polyamine pathway preventing protein degradation and facilitating cellular growth and proliferation. It is part of a regulatory complex that includes antizymes and affects the homeostasis of polyamines. This complex interplay significantly impacts the cellular environment and plays a role in maintaining cellular function influencing processes such as signal transduction and ion transport.

Pathways

AZIN2's manipulation of the polyamine pathway reveals its role in cellular growth regulation. It participates in the pathway alongside the arginine and proline metabolism influencing the balance of polyamines within cells. AZIN2 shares a relationship with ornithine decarboxylase and antizymes stabilizing polyamine levels critical for DNA stabilization and protein synthesis.

Dysregulation of AZIN2 can relate to cancer and metabolic syndrome. In cancer disrupted polyamine regulation can lead to uncontrolled cell proliferation; AZIN2 affects this through its interaction with antizymes and ornithine decarboxylase. In metabolic syndrome altered polyamine levels associate with energy homeostasis and can implicate AZIN2’s function in disease progression. Understanding these connections provides potential therapeutic avenues for managing such conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Antizyme inhibitor (AZI) protein that positively regulates ornithine decarboxylase (ODC) activity and polyamine uptake. AZI is an enzymatically inactive ODC homolog that counteracts the negative effect of ODC antizymes (AZs) OAZ1, OAZ2 and OAZ3 on ODC activity by competing with ODC for antizyme-binding (PubMed : 17900240). Inhibits antizyme-dependent ODC degradation and releases ODC monomers from their inactive complex with antizymes, leading to formation of the catalytically active ODC homodimer and restoring polyamine production (PubMed : 17900240). Participates in the morphological integrity of the trans-Golgi network (TGN) and functions as a regulator of intracellular secretory vesicle trafficking (PubMed : 20188728).
See full target information AZIN2
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Product promise

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For full details, please see our Terms & Conditions

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