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AB178678

Anti-Azurocidin antibody [EPR9502]

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Rabbit Recombinant Monoclonal Azurocidin antibody. Suitable for WB and reacts with Human samples.

View Alternative Names

Azurocidin, Cationic antimicrobial protein CAP37, Heparin-binding protein, HBP, hHBP, AZU1

1 Images
Western blot - Anti-Azurocidin antibody [EPR9502] (AB178678)
  • WB

Supplier Data

Western blot - Anti-Azurocidin antibody [EPR9502] (AB178678)

All lanes:

Western blot - Anti-Azurocidin antibody [EPR9502] (ab178678) at 1/1000 dilution

Lane 1:

Human spleen lysate at 10 µg

Lane 2:

U937 lysate at 10 µg

Predicted band size: 26 kDa

false

  • Carrier free

    Anti-Azurocidin antibody [EPR9502] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9502

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Azurocidin also known as heparin-binding protein or CAP37 is a serine protease homolog with a molecular mass of approximately 37 kDa. Unlike other serine proteases it doesn’t have protease activity due to specific structural changes in its catalytic triad. Azurocidin expresses mainly in azurophilic granules of neutrophils and monocytes. Upon activation these cells release it into the extracellular environment where it exerts its functions.
Biological function summary

Azurocidin plays a role in innate immune responses by promoting leukocyte recruitment and increasing vascular permeability. It does not form a part of any known enzyme complex though cooperates with other proteins to mount an effective defense against pathogens. Azurocidin works by acting as an antimicrobial agent demonstrating activity against a variety of gram-negative and gram-positive bacteria. It also modulates inflammatory responses and impacts cellular signaling.

Pathways

Azurocidin contributes to the innate immune signaling pathways and inflammation processes. It interacts with the inflammatory cascade by promoting the chemotaxis of monocytes and macrophages. Azurocidin relates to other inflammation modulating proteins such as cathepsin G and elastase which also originate from neutrophil granules. The interplay between these proteins forms a network that amplifies and sustains immune responses during infection or tissue injury.

Researchers associate azurocidin with conditions characterized by excessive inflammation such as sepsis and rheumatoid arthritis. During these conditions azurocidin's activity tends to increase contributing to pathophysiological developments. It connects particularly to proteinase 3 during inflammatory response in rheumatoid arthritis where they jointly participate in the degradation of cartilage and perpetuation of inflammation. In sepsis its role in increasing vascular permeability and promoting immune cell infiltration can intensify the inflammatory damage seen in patients.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.
See full target information Azurocidin
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com