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Rabbit Recombinant Monoclonal Azurocidin antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

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Images

Western blot - Anti-Azurocidin antibody [EPR9503] - BSA and Azide free (AB250556), expandable thumbnail
  • Western blot - Anti-Azurocidin antibody [EPR9503] - BSA and Azide free (AB250556), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Azurocidin antibody [EPR9503] - BSA and Azide free (AB250556), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Azurocidin antibody [EPR9503] - BSA and Azide free (AB250556), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Azurocidin antibody [EPR9503] - BSA and Azide free (AB250556), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.

Alternative names

Azurocidin, Cationic antimicrobial protein CAP37, Heparin-binding protein, HBP, hHBP, AZU1

Recommended products

Rabbit Recombinant Monoclonal Azurocidin antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR9503
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250556 is the carrier-free version of Anti-Azurocidin antibody [EPR9503] ab181989.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Azurocidin also known as heparin-binding protein or CAP37 is a serine protease homolog with a molecular mass of approximately 37 kDa. Unlike other serine proteases it doesn’t have protease activity due to specific structural changes in its catalytic triad. Azurocidin expresses mainly in azurophilic granules of neutrophils and monocytes. Upon activation these cells release it into the extracellular environment where it exerts its functions.

Biological function summary

Azurocidin plays a role in innate immune responses by promoting leukocyte recruitment and increasing vascular permeability. It does not form a part of any known enzyme complex though cooperates with other proteins to mount an effective defense against pathogens. Azurocidin works by acting as an antimicrobial agent demonstrating activity against a variety of gram-negative and gram-positive bacteria. It also modulates inflammatory responses and impacts cellular signaling.

Pathways

Azurocidin contributes to the innate immune signaling pathways and inflammation processes. It interacts with the inflammatory cascade by promoting the chemotaxis of monocytes and macrophages. Azurocidin relates to other inflammation modulating proteins such as cathepsin G and elastase which also originate from neutrophil granules. The interplay between these proteins forms a network that amplifies and sustains immune responses during infection or tissue injury.

Associated diseases and disorders

Researchers associate azurocidin with conditions characterized by excessive inflammation such as sepsis and rheumatoid arthritis. During these conditions azurocidin's activity tends to increase contributing to pathophysiological developments. It connects particularly to proteinase 3 during inflammatory response in rheumatoid arthritis where they jointly participate in the degradation of cartilage and perpetuation of inflammation. In sepsis its role in increasing vascular permeability and promoting immune cell infiltration can intensify the inflammatory damage seen in patients.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Azurocidin antibody [EPR9503] - BSA and Azide free (ab250556), expandable thumbnail

    Western blot - Anti-Azurocidin antibody [EPR9503] - BSA and Azide free (ab250556)

    This data was developed using Anti-Azurocidin antibody [EPR9503] ab181989, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Azurocidin antibody [EPR9503] (Anti-Azurocidin antibody [EPR9503] ab181989) at 1/10000 dilution

    Lane 1: HUVEC cell lysate at 20 µg

    Lane 2: 293 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 26 kDa

  • Western blot - Anti-Azurocidin antibody [EPR9503] - BSA and Azide free (ab250556), expandable thumbnail

    Western blot - Anti-Azurocidin antibody [EPR9503] - BSA and Azide free (ab250556)

    This data was developed using Anti-Azurocidin antibody [EPR9503] ab181989, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Azurocidin antibody [EPR9503] (Anti-Azurocidin antibody [EPR9503] ab181989) at 1/2000 dilution

    All lanes: Human spleen tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Azurocidin antibody [EPR9503] - BSA and Azide free (ab250556), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Azurocidin antibody [EPR9503] - BSA and Azide free (ab250556)

    This data was developed using Anti-Azurocidin antibody [EPR9503] ab181989, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Azurocidin with Anti-Azurocidin antibody [EPR9503] ab181989 at a 1/100 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Azurocidin antibody [EPR9503] - BSA and Azide free (ab250556), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Azurocidin antibody [EPR9503] - BSA and Azide free (ab250556)

    This data was developed using Anti-Azurocidin antibody [EPR9503] ab181989, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Azurocidin with Anti-Azurocidin antibody [EPR9503] ab181989 at a 1/100 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-Azurocidin antibody [EPR9503] - BSA and Azide free (ab250556), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Azurocidin antibody [EPR9503] - BSA and Azide free (ab250556)

    This data was developed using Anti-Azurocidin antibody [EPR9503] ab181989, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HUVEC cells labeling Azurocidin using Anti-Azurocidin antibody [EPR9503] ab181989 at a 1/40 dilution, or rabbit monoclonal IgG as isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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