AB12296

Anti-B MyB antibody

(1 Review)

(12 Publications )

Rabbit Polyclonal B MyB antibody. Suitable for WB and reacts with Human samples. Cited in 12 publications.

View Alternative Names

BMYB, MYBL2, Myb-related protein B, B-Myb, Myb-like protein 2

2 Images

Collaborator

Western blot - Anti-B MyB antibody (AB12296)

A : 293 HEK cells were transfected with negative control cDNA (pcDNA3 vector) or human B-Myb cDNA.

B : negative control cDNA (pcDNA3 vector) or human B-Myb cDNA were in vitro translated in rabbit reticulocyte lysate.

A band corresponding to B-Myb was detected (arrow) in all lanes except negative control lanes.

ab12296 dilution : 200 μg/ml.
Exposure was for 10 minutes.

A : 293 HEK cells were transfected with negative control cDNA (pcDNA3 vector) or human B-Myb cDNA.
B : negative control cDNA (pcDNA3 vector) or human B-Myb cDNA were in vitro translated in rabbit reticulocyte lysate.
A band corresponding to B-Myb was detected (arrow) in all lanes except negative control lanes.
ab12296 dilution : 200 µg/ml. Exposure was for 10 minutes.

All lanes:

Western blot - Anti-B MyB antibody (ab12296)

Predicted band size: 79 kDa

false

Exposure time: 10min

Michael Korenja and Alexander Brehm

Project

Western blot - Anti-B MyB antibody (AB12296)

All lanes:

Western blot - Anti-B MyB antibody (ab12296) at 1 µg/mL

All lanes:

HEK293 Wcl Transiently Overexpressing B-Myb at 5 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 122 kDa,79 kDa

Observed band size: 45 kDa,98 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The B MyB protein also known as MYBL2 or B-MYB is a transcription factor involved in cell cycle regulation. It belongs to the MYB family of proteins and has a molecular weight of approximately 92 kDa. This protein is expressed in a range of tissues including proliferating cells. B MyB's activity as a transcription factor involves binding to specific DNA sequences to regulate gene expression essential for cell cycle progression particularly in the S phase where it plays a role in DNA replication and mitosis.

Biological function summary

MYBL2 participates in processes important for normal cell cycle and proliferation. It does not appear to form part of a larger protein complex but regulates genes necessary for cell division independently. B MyB significantly impacts the expression of factors that facilitate the transition from G1 to S phase of the cell cycle impacting cellular growth and replication. Its function ensures that the cells adequately prepare for DNA synthesis and subsequent mitotic division.

Pathways

Various cell cycle regulatory pathways rely on MYBL2 for their correct functioning. B MyB influences the G1/S phase transition and is associated with the E2F transcription factor pathway. In this context it regulates E2F target genes necessary for DNA synthesis. B MyB interacts with other proteins such as CDK2 and cyclins to orchestrate a synchronized cell cycle progression ensuring that cells divide correctly and effectively.

B MyB is frequently associated with cancer development and progression notably in contexts where there is rapid cell proliferation such as in breast cancer and leukemia. Dysregulation of MYBL2 expression or its downstream targets can lead to uncontrolled cell division thereby contributing to oncogenesis. It associates with proteins such as p53 a pivotal tumor suppressor where alterations in their pathways can lead to malignancy. Researchers evaluate B MyB's expression and activity as potential markers for cancer diagnosis and as targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.

See full target information MYBL2

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:7733 PubMed38565963

2024

B-Myb deficiency boosts bortezomib-induced immunogenic cell death in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan-Jian Hui,Ting-Ting Yu,Liu-Gen Li,Xing-Chun Peng,Mao-Jun Di,Hui Liu,Wen-Long Gu,Tong-Fei Li,Kai-Liang Zhao,Wei-Xing Wang

Bioengineered 13:9019-9031 PubMed35341461

2022

Actin-binding Rho activating C-terminal like (ABRACL) transcriptionally regulated by MYB proto-oncogene like 2 (MYBL2) promotes the proliferation, invasion, migration and epithelial-mesenchymal transition of breast cancer cells.

Applications

Species

Human

Jie Li,Hui Chen

Cell death & disease 12:683 PubMed34234118

2021

A MYBL2 complex for RRM2 transactivation and the synthetic effect of MYBL2 knockdown with WEE1 inhibition against colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Liu,Lijuan Guo,Hongyan Qi,Meng Lou,Rui Wang,Boning Hai,Kailun Xu,Lijun Zhu,Yongfeng Ding,Chen Li,Lingdan Xie,Jing Shen,Xueping Xiang,Jimin Shao

American journal of cancer research 11:389-415 PubMed33575078

2021

CDCA8, targeted by MYBL2, promotes malignant progression and olaparib insensitivity in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Gonghua Qi,Chenyi Zhang,Hanlin Ma,Yingwei Li,Jiali Peng,Jingying Chen,Beihua Kong

Journal of translational medicine 19:4 PubMed33407591

2021

Extracellular vesicle-encapsulated microRNA-424 exerts inhibitory function in ovarian cancer by targeting MYB.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Li,Hongyan Xin,Lili Lu

The Prostate 80:674-686 PubMed32294305

2020

miR-30a inhibits androgen-independent growth of prostate cancer via targeting MYBL2, FOXD1, and SOX4.

Applications

Unspecified application

Species

Unspecified reactive species

Xinjun Li,Meng Jiao,Jing Hu,Mei Qi,Jing Zhang,Mingfeng Zhao,Hui Liu,Xueting Xiong,Xuesen Dong,Bo Han

Journal of neuropathology and experimental neurology 74:380-7 PubMed25756586

2015

Drosha inclusions are new components of dipeptide-repeat protein aggregates in FTLD-TDP and ALS C9orf72 expansion cases.

Applications

Unspecified application

Species

Unspecified reactive species

Sílvia Porta,Linda K Kwong,John Q Trojanowski,Virginia M-Y Lee

eLife 2:e00825 PubMed23878725

2013

MYBL2 is a sub-haploinsufficient tumor suppressor gene in myeloid malignancy.

Applications

Species

Human

Stefan Heinrichs,Lillian F Conover,Carlos E Bueso-Ramos,Outi Kilpivaara,Kristen Stevenson,Donna Neuberg,Mignon L Loh,Wen-Shu Wu,Scott J Rodig,Guillermo Garcia-Manero,Hagop M Kantarjian,A Thomas Look

Journal of cell science 126:3782-95 PubMed23750012

2013

Enhancement of c-Myc degradation by BLM helicase leads to delayed tumor initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Suruchika Chandra,Raina Priyadarshini,Vinoth Madhavan,Shweta Tikoo,Mansoor Hussain,Richa Mudgal,Priyanka Modi,Vivek Srivastava,Sagar Sengupta

RNA biology 10:287-300 PubMed23353574

2013

Large-scale screening identifies a novel microRNA, miR-15a-3p, which induces apoptosis in human cancer cell lines.

Applications

Species

Human

Aliaksandr Druz,Yu-Chi Chen,Rajarshi Guha,Michael Betenbaugh,Scott E Martin,Joseph Shiloach

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-B MyB antibody

Western blot - Anti-B MyB antibody (AB12296)

Western blot - Anti-B MyB antibody (AB12296)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise