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Rabbit Recombinant Monoclonal B MyB phospho T487 antibody. Suitable for IHC-P, WB, ICC/IF, Dot, Flow Cyt (Intra) and reacts with Human, Transfected cell line - Human, Synthetic peptide samples. Cited in 25 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B MyB (phospho T487) antibody [EPR2204Y] (AB76009), expandable thumbnail
  • Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] (AB76009), expandable thumbnail
  • Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] (AB76009), expandable thumbnail
  • Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] (AB76009), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-B MyB (phospho T487) antibody [EPR2204Y] (AB76009), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFDotFlow Cyt (Intra)
Human
Tested
Tested
Tested
Expected
Tested
Synthetic peptide
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Transfected cell line - Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/8000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Transfected cell line - Human, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species
Transfected cell line - Human, Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.

Alternative names

BMYB, MYBL2, Myb-related protein B, B-Myb, Myb-like protein 2

Recommended products

Rabbit Recombinant Monoclonal B MyB phospho T487 antibody. Suitable for IHC-P, WB, ICC/IF, Dot, Flow Cyt (Intra) and reacts with Human, Transfected cell line - Human, Synthetic peptide samples. Cited in 25 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2204Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The B MyB protein also known as MYBL2 or B-MYB is a transcription factor involved in cell cycle regulation. It belongs to the MYB family of proteins and has a molecular weight of approximately 92 kDa. This protein is expressed in a range of tissues including proliferating cells. B MyB's activity as a transcription factor involves binding to specific DNA sequences to regulate gene expression essential for cell cycle progression particularly in the S phase where it plays a role in DNA replication and mitosis.

Biological function summary

MYBL2 participates in processes important for normal cell cycle and proliferation. It does not appear to form part of a larger protein complex but regulates genes necessary for cell division independently. B MyB significantly impacts the expression of factors that facilitate the transition from G1 to S phase of the cell cycle impacting cellular growth and replication. Its function ensures that the cells adequately prepare for DNA synthesis and subsequent mitotic division.

Pathways

Various cell cycle regulatory pathways rely on MYBL2 for their correct functioning. B MyB influences the G1/S phase transition and is associated with the E2F transcription factor pathway. In this context it regulates E2F target genes necessary for DNA synthesis. B MyB interacts with other proteins such as CDK2 and cyclins to orchestrate a synchronized cell cycle progression ensuring that cells divide correctly and effectively.

Associated diseases and disorders

B MyB is frequently associated with cancer development and progression notably in contexts where there is rapid cell proliferation such as in breast cancer and leukemia. Dysregulation of MYBL2 expression or its downstream targets can lead to uncontrolled cell division thereby contributing to oncogenesis. It associates with proteins such as p53 a pivotal tumor suppressor where alterations in their pathways can lead to malignancy. Researchers evaluate B MyB's expression and activity as potential markers for cancer diagnosis and as targets for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009)

    Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue sections labelling B MyB (phospho T487) with ab76009 at 1/8000 dilution. The section was incubated with ab76009 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
    Nuclear staining on Human colon cancer tissue without alkaline phosphatase treatment (Image A); No signal was detected when tissues were treated with alkaline phosphatase (Image B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009), expandable thumbnail

    Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009)

    Blocking buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration: 5% NFDM/TBST

    Lanes 1 - 2: Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free ab232521) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) nuclear fraction lysate at 15 µg

    Lane 2: HeLa nuclear fraction lysate treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 79 kDa

    Observed band size: 100 kDa

    Exposure time: 120s

  • Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009), expandable thumbnail

    Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009)

    Blocking buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration: 5% NFDM/TBST

    Lanes 1 - 2: Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free ab232521) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) nuclear fraction lysate at 15 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) without nuclear fraction lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 79 kDa

    Observed band size: 100 kDa

    Exposure time: 60s

  • Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009), expandable thumbnail

    Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009)

    Blocking buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration: 5% NFDM/TBST

    Lanes 1 - 2: Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free ab232521) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009) at 1/1000 dilution

    Lane 1: 293T (Human embryonic kidney epithelial cell) transfected with empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 2: 293T transfected with human B MyB (WT) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 79 kDa

    Observed band size: 100 kDa

    Exposure time: 20s

  • Immunocytochemistry/ Immunofluorescence - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling B MyB (phospho T487) with primary antibody anti-B MyB (phospho T487) (ab76009) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody at 1/1000 dilution. Confocal image showing nuclear staining in HeLa cells and no staining in HeLa cells with Alkaline Phosphatase treatment 37℃ for 1 hour. Anti-alpha Tubulin antibody (DM1A) - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) was used to counterstain tubulin at 1/200 dilution. The nuclear counter stain is DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009)

    Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue sections labelling B MyB (phospho T487) with ab76009 at 1/8000 dilution. The section was incubated with ab76009 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
    Nuclear staining on Human lung cancer tissue without alkaline phosphatase treatment (Image A); No signal was detected when tissues were treated with alkaline phosphatase (Image B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Flow Cytometry (Intracellular) - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells treated with Alkaline Phosphatase overnight (Left) and untreated HeLa cells (Right) labeling B MyB (phospho T487) with ab76009 at 1/1000 dilution (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Dot Blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009), expandable thumbnail

    Dot Blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009)

    Dot blot analysis of B MyB (pT487) phospho peptide (lane 1) and B MyB non-phospho peptide (lane 2) with ab76009 at a 1/1000 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at a dilution of 1/20,000.
    Blocking and dilution buffer: 5% NFDM/TBST
    Exposure time: 3 minutes

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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