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Rabbit Recombinant Monoclonal B MyB phospho T487 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Dot, Flow Cyt (Intra) and reacts with Human, Transfected cell line - Human, Synthetic peptide samples.

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Images

Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (AB232521), expandable thumbnail
  • Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (AB232521), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (AB232521), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (AB232521), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (AB232521), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFDotFlow Cyt (Intra)
Human
Tested
Tested
Tested
Expected
Tested
Synthetic peptide
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Transfected cell line - Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Transfected cell line - Human, Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human, Transfected cell line - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Human, Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species

Transfected cell line - Human, Synthetic peptide

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.

Alternative names

Myb-related protein B, B-Myb, Myb-like protein 2, MYBL2, BMYB

Recommended products

Rabbit Recombinant Monoclonal B MyB phospho T487 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Dot, Flow Cyt (Intra) and reacts with Human, Transfected cell line - Human, Synthetic peptide samples.

Alternative names

Myb-related protein B, B-Myb, Myb-like protein 2, MYBL2, BMYB

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR2204Y

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab232521 is the carrier-free version of Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The B MyB protein also known as MYBL2 or B-MYB is a transcription factor involved in cell cycle regulation. It belongs to the MYB family of proteins and has a molecular weight of approximately 92 kDa. This protein is expressed in a range of tissues including proliferating cells. B MyB's activity as a transcription factor involves binding to specific DNA sequences to regulate gene expression essential for cell cycle progression particularly in the S phase where it plays a role in DNA replication and mitosis.

Biological function summary

MYBL2 participates in processes important for normal cell cycle and proliferation. It does not appear to form part of a larger protein complex but regulates genes necessary for cell division independently. B MyB significantly impacts the expression of factors that facilitate the transition from G1 to S phase of the cell cycle impacting cellular growth and replication. Its function ensures that the cells adequately prepare for DNA synthesis and subsequent mitotic division.

Pathways

Various cell cycle regulatory pathways rely on MYBL2 for their correct functioning. B MyB influences the G1/S phase transition and is associated with the E2F transcription factor pathway. In this context it regulates E2F target genes necessary for DNA synthesis. B MyB interacts with other proteins such as CDK2 and cyclins to orchestrate a synchronized cell cycle progression ensuring that cells divide correctly and effectively.

Associated diseases and disorders

B MyB is frequently associated with cancer development and progression notably in contexts where there is rapid cell proliferation such as in breast cancer and leukemia. Dysregulation of MYBL2 expression or its downstream targets can lead to uncontrolled cell division thereby contributing to oncogenesis. It associates with proteins such as p53 a pivotal tumor suppressor where alterations in their pathways can lead to malignancy. Researchers evaluate B MyB's expression and activity as potential markers for cancer diagnosis and as targets for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521), expandable thumbnail

    Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521)

    Blocking buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration: 5% NFDM/TBST
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009).

    Lanes 1 - 2: Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] (Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) nuclear fraction lysate at 15 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) without nuclear fraction lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 79 kDa

    Observed band size: 100 kDa

    Exposure time: 60s

    Blocking buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521), expandable thumbnail

    Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521)

    Blocking buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration: 5% NFDM/TBST
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009).

    Lanes 1 - 2: Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] (Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009) at 1/1000 dilution

    Lane 1: 293T (Human embryonic kidney epithelial cell) transfected with empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 2: 293T transfected with human B MyB (WT) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 79 kDa

    Observed band size: 100 kDa

    Exposure time: 20s

    Blocking buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling B MyB (phospho T487) with primary antibody anti-B MyB (phospho T487) (Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody at 1/1000 dilution. Confocal image showing nuclear staining in HeLa cells and no staining in HeLa cells with Alkaline Phosphatase treatment 37°C for 1 hour. Anti-alpha Tubulin antibody (DM1A) - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) was used to counterstain tubulin at 1/200 dilution. The nuclear counter stain is DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521)

    Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue sections labelling B MyB (phospho T487) with Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009 at 1/8000 dilution. The section was incubated with Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
    Nuclear staining on Human lung cancer tissue without alkaline phosphatase treatment (Image A); No signal was detected when tissues were treated with alkaline phosphatase (Image B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009).

  • Flow Cytometry (Intracellular) - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells treated with Alkaline Phosphatase overnight (Left) and untreated HeLa cells (Right) labeling B MyB (phospho T487) with Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009 at 1/1000 dilution (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009).

  • Dot Blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521), expandable thumbnail

    Dot Blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521)

    Dot blot analysis of B MyB (pT487) phospho peptide (lane 1) and B MyB non-phospho peptide (lane 2) with Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009 at a 1/1000 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at a dilution of 1/20,000.
    Blocking and dilution buffer: 5% NFDM/TBST
    Exposure time: 3 minutes

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521)

    Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue sections labelling B MyB (phospho T487) with Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009 at 1/8000 dilution. The section was incubated with Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
    Nuclear staining on Human colon cancer tissue without alkaline phosphatase treatment (Image A); No signal was detected when tissues were treated with alkaline phosphatase (Image B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009).

  • Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521), expandable thumbnail

    Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521)

    Blocking buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration: 5% NFDM/TBST
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009).

    Lanes 1 - 2: Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Anti-B MyB (phospho T487) antibody [EPR2204Y] (Anti-B MyB (phospho T487) antibody [EPR2204Y] ab76009) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) nuclear fraction lysate at 15 µg

    Lane 2: HeLa nuclear fraction lysate treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 79 kDa

    Observed band size: 100 kDa

    Exposure time: 120s

    Blocking buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration: 5% NFDM/TBST

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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