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AB247291

Anti-b-Myc antibody [EP643Y] - BSA and Azide free

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Rabbit Recombinant Monoclonal b-Myc antibody. Carrier free. Suitable for WB and reacts with Rat samples.

View Alternative Names

Bmyc, Mycb, Protein B-Myc

1 Images
Western blot - Anti-b-Myc antibody [EP643Y] - BSA and Azide free (AB247291)
  • WB

Unknown

Western blot - Anti-b-Myc antibody [EP643Y] - BSA and Azide free (AB247291)

This data was developed using ab40798, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-b-Myc antibody [EP643Y] (<a href='/en-us/products/primary-antibodies/b-myc-antibody-ep643y-ab40798'>ab40798</a>) at 1/500 dilution

Lane 1:

Rat Brain

Lane 2:

PC12

Predicted band size: 18.3 kDa

Observed band size: 20 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP643Y

Isotype

IgG

Carrier free

Yes

Reacts with

Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab247291 is the carrier-free version of ab40798.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

B-Myc also known as B-Myc or MYCL is a member of the Myc family of transcription factors. It is an essential regulator in cellular processes like growth and differentiation. The molecular weight of b-Myc is approximately 24 kDa. This protein acts as a transcriptional repressor and is mainly expressed in tissues such as the mammary gland lung and prostate. It plays a part in suppressing the Myc-mediated gene expression by competing for the same DNA binding sites thereby offering a balance to the functions of other Myc proteins.
Biological function summary

B-Myc functions to modulate transcriptional activities that are necessary for cellular homeostasis. It can associate with other proteins and is often considered part of transcriptional repression complexes. These complexes contribute to regulating gene expression achieving this by directly binding to target DNA sequences or interacting with other transcription factors and co-repressors. Its activities significantly impact cellular differentiation and proliferation providing checks and balances against other members of the Myc family that promote cellular growth.

Pathways

B-Myc is involved in pathways that play a critical role in cell cycle regulation and apoptosis. It actively participates in the Myc signaling pathway where its presence helps counterbalance the more proliferative effects of c-Myc and N-Myc. By doing so it interacts with proteins like Max enabling the formation of different dimers with varying transcriptional roles. Additionally b-Myc's activity within these pathways suggests an intriguing role in modulating cellular responses to growth signals and stressors.

B-Myc's expression and functioning relate to cancer development particularly in breast and prostate cancers. Its capacity to restrain Myc-driven transcriptional programs implies that deviations in b-Myc expression can lead to unregulated cell proliferation and tumorigenesis. In cancerous contexts alterations in b-Myc's interactions with proteins such as EZH2 a component of the PRC2 complex highlight its potential implication in the disruption of normal cellular stability. Understanding b-Myc's role in these diseases offers insights into targeted therapeutic strategies where inhibiting or modulating its function could yield beneficial outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Seems to act as an inhibitor of cellular proliferation.
See full target information Mycb
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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