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AB137492

Anti-B19 antibody

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(2 Publications)

Rabbit Polyclonal B19 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human HUNK aa 650 to C-terminus.

View Alternative Names

MAKV, HUNK, Hormonally up-regulated neu tumor-associated kinase, B19, Serine/threonine-protein kinase MAK-V

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B19 antibody (AB137492)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B19 antibody (AB137492)

Immunohistochemical analysis of paraffin-embedded Human OVCA xenograft tissue labelling B19 with ab137492 at 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-B19 antibody (AB137492)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-B19 antibody (AB137492)

Immunofluorescence analysis of paraformaldehyde-fixed A549 cells labelling B19 with ab137492 at 1/200 dilution. The lower image is costained with Hoechst 33342.

Western blot - Anti-B19 antibody (AB137492)
  • WB

Unknown

Western blot - Anti-B19 antibody (AB137492)

7.5% SDS PAGE

All lanes:

Western blot - Anti-B19 antibody (ab137492) at 1/1000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 80 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human HUNK aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P57058

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>or Tris-EDTA buffer pH 8</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Human parvovirus B19 also known simply as B19 is a small DNA virus with a linear genome and a non-enveloped capsid. The virus itself is approximately 5.6 kilodaltons in mass. B19 targets and infects erythroid progenitor cells in the bone marrow which are critical for red blood cell development. Its expression occurs primarily in tissues with rapid cell turnover such as the bone marrow and B19 is also detectable in fetal liver tissues.
Biological function summary

Human parvovirus B19 acts by binding to the P antigen on susceptible cells particularly erythroid precursors. This interaction facilitates the virus's entry into the cell where it replicates and inhibits cellular DNA synthesis potentially causing cell apoptosis. The virus does not typically form part of a protein complex within the host cell but manipulates host cellular machinery to replicate its own DNA effectively. This mechanism highlights B19's influence in the development of certain anemic conditions such as transient aplastic crisis in patients with hemolytic disorders.

Pathways

The infection mechanism of human parvovirus B19 involves several cellular pathways including those associated with cell cycle regulation and apoptosis. Notably B19 impacts the retinoblastoma protein (pRb) related pathways which are important in controlling cell cycle progression. Furthermore the virus interferes with tumor suppressor protein p53 disrupting pathways related to DNA damage response. These interactions emphasize the virus's adeptness at manipulating host cell controls to ensure successful replication.

Infection with human parvovirus B19 has associations with erythema infectiosum and hydrops fetalis. In individuals with compromised immune systems or those with existing hemolytic anemia B19 infection can aggravate these conditions as the virus exacerbates the reduction of erythroid progenitor cells. The virus shares a relationship with the TNF-alpha protein particularly in inflammatory and autoimmune responses seen in parvovirus-associated arthralgia. This connection underlines the potential for B19 to contribute to a range of pathological conditions through direct and indirect interactions within the host.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information HUNK
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35805907

2022

In Vitro and Ectopic In Vivo Studies toward the Utilization of Isolated Human Nasal Chondrocytes for Single-Stage Arthroscopic Cartilage Regeneration Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Gyözö Lehoczky,Raluca Elena Trofin,Queralt Vallmajo-Martin,Shikha Chawla,Karoliina Pelttari,Marcus Mumme,Martin Haug,Christian Egloff,Marcel Jakob,Martin Ehrbar,Ivan Martin,Andrea Barbero

Cartilage 13:68S-81S PubMed32959685

2020

Comparison of Human Articular Cartilage Tissue and Chondrocytes Isolated from Peripheral versus Central Regions of Traumatic Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Acevedo,Lukas Iselin,Majoska H M Berkelaar,Gian M Salzmann,Francine Wolf,Sandra Feliciano,Nicole Vogel,Geert Pagenstert,Ivan Martin,Karoliina Pelttari,Andrea Barbero,Markus P Arnold
View all publications
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