Rabbit Polyclonal B3GALNT1 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human B3GALNT1 aa 1-250.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human B3GALNT1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link O75752
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Species Mouse | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Transfers N-acetylgalactosamine onto globotriaosylceramide (PubMed:10993897). Plays a critical role in preimplantation stage embryonic development (By similarity).
Alternative names
B3GALT3, UNQ531/PRO1074, B3GALNT1, Beta-3-Gx-T3, Galactosylgalactosylglucosylceramide beta-D-acetyl-galactosaminyltransferase, Globoside synthase, Beta3Gal-T3, Beta3GalT3, b3Gal-T3
Recommended products
Rabbit Polyclonal B3GALNT1 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human B3GALNT1 aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human B3GALNT1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link O75752
- Purification technique
- Affinity purification Immunogen
- Reconstitution
- Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
{B3GALNT1} also known as beta-13-N-acetylgalactosaminyltransferase 1 is an enzyme that catalyzes the addition of N-acetylgalactosamine in a beta-13 linkage to its substrate. The enzyme has a molecular weight of approximately 60 kDa. It plays a significant role in the biosynthesis of certain glycoproteins and glycolipids. {B3GALNT1} is expressed in various tissues with higher levels observed in the brain and the central nervous system.
- Biological function summary
The {B3GALNT1} enzyme contributes to the synthesis of Ganglioside GM2 an important component of neuronal membranes. It is not part of a complex but interacts with other enzymes in the sialylation pathways to ensure the correct structure of gangliosides. These interactions stabilize cellular membranes and facilitate cell signaling. Proper function of {B3GALNT1} ensures the integrity of cell communication and adhesion.
- Pathways
{B3GALNT1} plays a role in the O-glycan biosynthesis pathway. This pathway contributes to the formation of glycoproteins essential for cellular interactions and signal transduction. The enzyme also participates in the metabolism of glycosphingolipids which connects it to proteins like ST3GAL2 that further modify gangliosides. These pathways highlight its importance in maintaining cellular homeostasis and health.
- Associated diseases and disorders
Mutations or dysregulation of {B3GALNT1} are linked to GM2 gangliosidosis a neurodegenerative disorder. Altered enzymatic activity can also associate with cancer as abnormal glycosylation affects tumor progression and metastasis. {B3GALNT1} interacts with proteins such as HEXA in these diseases highlighting its significance in pathological conditions. Understanding {B3GALNT1}'s roles aids in the development of therapeutic approaches for these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GALNT1 antibody (ab176891)
Immunohistochemical analysis of formalin fixed and paraffin embedded Human fetal colon labeling B3GALNT1 with ab176891 at 1/00 dilution.
Western blot - Anti-B3GALNT1 antibody (ab176891)
All lanes: Western blot - Anti-B3GALNT1 antibody (ab176891) at 1/200 dilution
Lane 1: Rat muscle lysate
Lane 2: Mouse brain lysate
Developed using the ECL technique.
Predicted band size: 40 kDa
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