AB249506

Anti-B3GALT4 antibody [EPR11650] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal B3GALT4 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

GALT4, B3GALT4, Beta3Gal-T4, Beta3GalT4, GalT4, b3Gal-T4, Gal-T2, Ganglioside galactosyltransferase

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GALT4 antibody [EPR11650] - BSA and Azide free (AB249506)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GALT4 antibody [EPR11650] - BSA and Azide free (AB249506)

This data was developed using ab169759, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling B3GALT4 with ab169759 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GALT4 antibody [EPR11650] - BSA and Azide free (AB249506)

This data was developed using ab169759, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling B3GALT4 with ab169759 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-B3GALT4 antibody [EPR11650] - BSA and Azide free (AB249506)

This data was developed using ab169759, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-B3GALT4 antibody [EPR11650] (<a href='/en-us/products/primary-antibodies/b3galt4-antibody-epr11650-ab169759'>ab169759</a>) at 1/10000 dilution

Lane 1:

Fetal tissue lysate at 10 µg

Lane 2:

PC-3 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

U87-MG cell lysate at 10 µg

Predicted band size: 42 kDa

false

Flow Cytometry (Intracellular) - Anti-B3GALT4 antibody [EPR11650] - BSA and Azide free (AB249506)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-B3GALT4 antibody [EPR11650] - BSA and Azide free (AB249506)

This data was developed using ab169759, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling B3GALT4 with ab169759 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11650

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab249506 is the carrier-free version of ab169759.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

B3GALT4 also known as beta-13-galactosyltransferase-4 is a glycosyltransferase enzyme that plays an important role in glycosylation processes. This enzyme facilitates the transfer of galactose from UDP-galactose to the terminal N-acetylglucosamine of glycoproteins and glycolipids forming a beta-13 bond. B3GALT4 has a molecular mass of approximately 42 kDa. Expression of B3GALT4 occurs in various tissues including the brain liver and kidney indicating its extensive involvement across different physiological systems.

Biological function summary

The enzyme B3GALT4 contributes to the synthesis of glycan structures. It participates in the glycosphingolipid biosynthesis pathway where it has involvement in the addition of galactose to lactosylceramide. By doing so B3GALT4 helps in transforming lactosylceramide into more complex glycolipids which are components of cell membranes and mediate cell-cell interactions. This enzyme is not known as part of a larger complex but it acts independently within the glycosylation machinery.

Pathways

B3GALT4 functions within the glycosphingolipid metabolism and the N-glycan biosynthesis pathways. It contributes to the complexity and diversity of glycan structures that are key in these pathways. B3GALT4 interacts with other glycosyltransferases such as beta-14-galactosyltransferase in orchestrating the stepwise extension of oligosaccharide chains. Understanding these pathways offers insight into how B3GALT4 contributes to essential cellular functions like cell signaling and immune response.

B3GALT4 dysregulation associates with GM1 gangliosidosis and Morquio syndrome type B. GM1 gangliosidosis results from the accumulation of GM1 gangliosides due to insufficient breakdown often influenced by abnormal glycosylation from B3GALT4 activity. This disorder connects B3GALT4 with the lysosomal enzyme beta-galactosidase which is directly involved in degrading glycolipids. In Morquio syndrome type B mutations impacting glycosylation pathways suggest an indirect link to B3GALT4's enzymatic processes ultimately contributing to skeletal abnormalities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in GM1/GD1B/GA1 ganglioside biosynthesis.

See full target information B3GALT4

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-B3GALT4 antibody [EPR11650] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GALT4 antibody [EPR11650] - BSA and Azide free (AB249506)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GALT4 antibody [EPR11650] - BSA and Azide free (AB249506)

Western blot - Anti-B3GALT4 antibody [EPR11650] - BSA and Azide free (AB249506)

Flow Cytometry (Intracellular) - Anti-B3GALT4 antibody [EPR11650] - BSA and Azide free (AB249506)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise