AB199156

Anti-B3GAT1 antibody

Name: Anti-B3GAT1 antibody (ab199156) | Abcam
Brand: Abcam Limited
SKU: AB199156
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal B3GAT1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human B3GAT1.

View Alternative Names

GLCATP, B3GAT1, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Glucuronosyltransferase P, GlcAT-P, GlcUAT-P

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GAT1 antibody (AB199156)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GAT1 antibody (AB199156)

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling B3GAT1 with ab199156 at 1/40 dilution.

Supplier Data

Western blot - Anti-B3GAT1 antibody (AB199156)

Gel : 10% SDS-PAGE

All lanes:

Western blot - Anti-B3GAT1 antibody (ab199156) at 1/400 dilution

All lanes:

Mouse brain tissue lysate at 40 µg

Predicted band size: 38 kDa

false

Exposure time: 20s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human B3GAT1. The exact immunogen used to generate this antibody is proprietary information.

Q9P2W7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The B3GAT1 protein also known as CD57 or HNK-1 functions mechanically as a β-13-glucuronyltransferase. It has a mass of approximately 55 kDa. B3GAT1 localizes mainly in the Golgi apparatus and is predominantly expressed in neural and immune tissues. The protein plays an important role in the biosynthesis of the HNK-1 carbohydrate epitope which is significant for cellular communication and molecular interactions in the nervous system.

Biological function summary

B3GAT1 participates in the generation of specific carbohydrate structures that contribute to cell-cell adhesion processes. It is a part of a larger complex of glycosyltransferases that include enzymes like β3GalT1 and ST3GalT6 which together help build complex glycan chains. By modifying glycoproteins and glycolipids B3GAT1 influences neural connectivity and immune response modulation.

Pathways

B3GAT1 influences with its role in the glycosphingolipid biosynthesis pathway and the neural crest cell migration pathway. In glycosphingolipid biosynthesis its activity is essential for creating carbohydrate polymer structures linking it with proteins like β3GalT2. It also interacts with NCAM1 within the neural crest cell migration pathway impacting neural development and plasticity.

Abnormal expression or mutations in B3GAT1 link to diseases such as Alzheimer's as the HNK-1 epitope expression correlates with neuronal impairment. In cancers altered B3GAT1 glycosylation patterns associate with tumor progression and immune escape mechanisms where its interaction with CD56 plays a role in modulating immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity : stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.

See full target information B3GAT1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 299:105128 PubMed37543361

2023

Brain-specific glycosylation enzyme GnT-IX maintains levels of protein tyrosine phosphatase receptor PTPRZ, thereby mediating glioma growth.

Applications

Unspecified application

Species

Unspecified reactive species

Kenichiro Nagai,Yui Muto,Saori Miura,Kazuto Takahashi,Yu Naruse,Ryo Hiruta,Yuko Hashimoto,Miwa Uzuki,Yoshimi Haga,Risa Fujii,Koji Ueda,Yasushi Kawaguchi,Masazumi Fujii,Shinobu Kitazume

Journal of oncology 2021:1901191 PubMed34868311

2021

Potential Clinical Value of 5-Hydroxytryptamine Receptor 3C as a Prognostic Biomarker for Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiun-Rung Chen,Ming-Shyan Huang,Yi-Chen Lee,Min-His Lin,Yi-Fang Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-B3GAT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GAT1 antibody (AB199156)

Western blot - Anti-B3GAT1 antibody (AB199156)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise