AB221756

Anti-B3GAT1 antibody

Name: Anti-B3GAT1 antibody (ab221756) | Abcam
Brand: Abcam Limited
SKU: AB221756
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal B3GAT1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human B3GAT1 aa 1-100.

View Alternative Names

GLCATP, B3GAT1, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Glucuronosyltransferase P, GlcAT-P, GlcUAT-P

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-B3GAT1 antibody (AB221756)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-B3GAT1 antibody (AB221756)

PFA-fixed, Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained for B3GAT1 (green) using ab221756 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human B3GAT1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9P2W7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The B3GAT1 protein also known as CD57 or HNK-1 functions mechanically as a β-13-glucuronyltransferase. It has a mass of approximately 55 kDa. B3GAT1 localizes mainly in the Golgi apparatus and is predominantly expressed in neural and immune tissues. The protein plays an important role in the biosynthesis of the HNK-1 carbohydrate epitope which is significant for cellular communication and molecular interactions in the nervous system.

Biological function summary

B3GAT1 participates in the generation of specific carbohydrate structures that contribute to cell-cell adhesion processes. It is a part of a larger complex of glycosyltransferases that include enzymes like β3GalT1 and ST3GalT6 which together help build complex glycan chains. By modifying glycoproteins and glycolipids B3GAT1 influences neural connectivity and immune response modulation.

Pathways

B3GAT1 influences with its role in the glycosphingolipid biosynthesis pathway and the neural crest cell migration pathway. In glycosphingolipid biosynthesis its activity is essential for creating carbohydrate polymer structures linking it with proteins like β3GalT2. It also interacts with NCAM1 within the neural crest cell migration pathway impacting neural development and plasticity.

Abnormal expression or mutations in B3GAT1 link to diseases such as Alzheimer's as the HNK-1 epitope expression correlates with neuronal impairment. In cancers altered B3GAT1 glycosylation patterns associate with tumor progression and immune escape mechanisms where its interaction with CD56 plays a role in modulating immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity : stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.

See full target information B3GAT1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports methods 2:100367 PubMed36590694

2022

-derived medium spiny neurons recapitulate human striatal development and complexity at single-cell resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Conforti,Vittoria Dickinson Bocchi,Ilaria Campus,Linda Scaramuzza,Maura Galimberti,Tiziana Lischetti,Francesca Talpo,Matteo Pedrazzoli,Alessio Murgia,Ivan Ferrari,Chiara Cordiglieri,Alessandra Fasciani,Ernest Arenas,Dan Felsenfeld,Gerardo Biella,Dario Besusso,Elena Cattaneo

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