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AB327128

Anti-B3GAT1 Antibody [CL9500]

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Mouse Monoclonal B3GAT1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human B3GAT1.

View Alternative Names

GLCATP, B3GAT1, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Glucuronosyltransferase P, GlcAT-P, GlcUAT-P

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GAT1 Antibody [CL9500] (AB327128)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GAT1 Antibody [CL9500] (AB327128)

Immunohistochemical staining of human duodenum using ab327128 at 1 : 200–1 : 500 dilution, showing moderate cytoplasmic positivity in lymphoid cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GAT1 Antibody [CL9500] (AB327128)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GAT1 Antibody [CL9500] (AB327128)

Immunohistochemical staining of human tonsil using ab327128 at 1 : 200–1 : 500 dilution, showing strong cytoplasmic positivity in a small subset of lymphoid cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GAT1 Antibody [CL9500] (AB327128)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GAT1 Antibody [CL9500] (AB327128)

Immunohistochemical staining of human renal cancer using ab327128 at 1 : 200–1 : 500 dilution, showing moderate cytoplasmic positivity in lymphoid cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GAT1 Antibody [CL9500] (AB327128)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GAT1 Antibody [CL9500] (AB327128)

Immunohistochemical staining of human prostate using ab327128 at 1 : 200–1 : 500 dilution, showing moderate to strong staining of secretion.

Western blot - Anti-B3GAT1 Antibody [CL9500] (AB327128)
  • WB

Supplier Data

Western blot - Anti-B3GAT1 Antibody [CL9500] (AB327128)

Western blot analysis in human cerebral cortex tissue using ab327128 at 1 µg/ml.

All lanes:

Western blot - Anti-B3GAT1 Antibody [CL9500] (ab327128)

All lanes:

Human cerebral cortex tissue

Predicted band size: 38.26 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GAT1 Antibody [CL9500] (AB327128)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GAT1 Antibody [CL9500] (AB327128)

Immunohistochemical staining of human skeletal muscle using ab327128 at 1 : 200–1 : 500 dilution, showing no positivity in myocytes as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL9500

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human B3GAT1.

Q9P2W7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity : stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
See full target information B3GAT1
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Product promise

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