AB203123

Anti-B3GAT1 antibody - N-terminal

Name: Anti-B3GAT1 antibody - N-terminal (ab203123) | Abcam
Brand: Abcam Limited
SKU: AB203123
Price: 520 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal B3GAT1 antibody. N-terminal. Suitable for IHC-P and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human B3GAT1 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

GLCATP, B3GAT1, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Glucuronosyltransferase P, GlcAT-P, GlcUAT-P

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GAT1 antibody - N-terminal (AB203123)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GAT1 antibody - N-terminal (AB203123)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse lung tissue labeling B3GAT1 with ab203123 at 1/100 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human B3GAT1 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9P2W7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>(or 1/50 - 1/200 using a fluorescent secondary antibody).</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The B3GAT1 protein also known as CD57 or HNK-1 functions mechanically as a β-13-glucuronyltransferase. It has a mass of approximately 55 kDa. B3GAT1 localizes mainly in the Golgi apparatus and is predominantly expressed in neural and immune tissues. The protein plays an important role in the biosynthesis of the HNK-1 carbohydrate epitope which is significant for cellular communication and molecular interactions in the nervous system.

Biological function summary

B3GAT1 participates in the generation of specific carbohydrate structures that contribute to cell-cell adhesion processes. It is a part of a larger complex of glycosyltransferases that include enzymes like β3GalT1 and ST3GalT6 which together help build complex glycan chains. By modifying glycoproteins and glycolipids B3GAT1 influences neural connectivity and immune response modulation.

Pathways

B3GAT1 influences with its role in the glycosphingolipid biosynthesis pathway and the neural crest cell migration pathway. In glycosphingolipid biosynthesis its activity is essential for creating carbohydrate polymer structures linking it with proteins like β3GalT2. It also interacts with NCAM1 within the neural crest cell migration pathway impacting neural development and plasticity.

Abnormal expression or mutations in B3GAT1 link to diseases such as Alzheimer's as the HNK-1 epitope expression correlates with neuronal impairment. In cancers altered B3GAT1 glycosylation patterns associate with tumor progression and immune escape mechanisms where its interaction with CD56 plays a role in modulating immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity : stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.

See full target information B3GAT1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutics 13: PubMed34683924

2021

Bi-Functional Peptides as a New Therapeutic Tool for Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Savier,Lorena Simon-Gracia,Frederic Charlotte,Pierre Tuffery,Tambet Teesalu,Olivier Scatton,Angelita Rebollo

International journal of cancer 146:192-207 PubMed31107974

2019

Wnt/IL-1β/IL-8 autocrine circuitries control chemoresistance in mesothelioma initiating cells by inducing ABCB5.

Applications

Unspecified application

Species

Unspecified reactive species

Vladan Milosevic,Joanna Kopecka,Iris C Salaroglio,Roberta Libener,Francesca Napoli,Stefania Izzo,Sara Orecchia,Preeta Ananthanarayanan,Paolo Bironzo,Federica Grosso,Fabrizio Tabbò,Valentina Comunanza,Teodora Alexa-Stratulat,Federico Bussolino,Luisella Righi,Silvia Novello,Giorgio V Scagliotti,Chiara Riganti

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-B3GAT1 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GAT1 antibody - N-terminal (AB203123)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise