Rabbit Polyclonal B3GNT antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human B4GAT1 aa 150 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human B4GAT1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O43505
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Cow | Predicted |
| Orangutan | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Orangutan | Dilution info - | Notes - |
Target data
Function
Beta-1,4-glucuronyltransferase involved in O-mannosylation of alpha-dystroglycan (DAG1) (PubMed:19587235, PubMed:23359570, PubMed:25279697, PubMed:25279699). Transfers a glucuronic acid (GlcA) residue onto a xylose (Xyl) acceptor to produce the glucuronyl-beta-1,4-xylose-beta disaccharide primer, which is further elongated by LARGE1, during synthesis of phosphorylated O-mannosyl glycan (PubMed:25279697, PubMed:25279699). Phosphorylated O-mannosyl glycan is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (PubMed:25279697, PubMed:25279699). Required for axon guidance; via its function in O-mannosylation of alpha-dystroglycan (DAG1) (By similarity).
Alternative names
B3GNT1, B3GNT6, B4GAT1, Poly-N-acetyllactosamine extension enzyme, iGnT
Recommended products
Rabbit Polyclonal B3GNT antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human B4GAT1 aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human B4GAT1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O43505
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity greater than 95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
B3GNT also known as Beta-13-N-acetylglucosaminyltransferase is an enzyme that facilitates the transfer of N-acetylglucosamine from UDP-GlcNAc to various substrates. It often involves the synthesis of poly-N-acetyllactosamine chains. B3GNT family proteins weigh around 45 kDa although variations might exist. This enzyme mostly expresses in tissues such as the liver spleen and lungs. During mechanical processes B3GNT operates in the Golgi apparatus and actively participates in glycosylation processes.
- Biological function summary
B3GNT enzymes play an important role in creating and modifying glycan structures on glycoproteins and glycolipids. Complex assemblies form with other glycosyltransferases for the efficient construction and regulation of glycan chains. These glycan structures are important for cellular processes like cell adhesion migration and signal transduction. By shaping these vital cellular events B3GNT contributes to fundamental biological functions and cell interactions.
- Pathways
B3GNT performs essential functions in the glycosphingolipid biosynthesis and Notch signaling pathways. These pathways govern cell fate determination morphogenesis and differentiation. B3GNT interacts with various proteins including fucosyltransferases and sialyltransferases during the synthesis of complex glycan structures. The Notch signaling pathway also involves proteins such as Notch receptors and ligands where B3GNT influences receptor-ligand interactions through glycosylation modifications impacting the signaling outcomes.
- Associated diseases and disorders
B3GNT dysfunction correlates with conditions like cancer and congenital disorders of glycosylation. Changes in glycan structures due to altered B3GNT activity often link with tumorigenesis and cancer metastasis. B3GNT's relationship with proteins like E-cadherin in cancer can affect E-cadherin-mediated cell adhesion promoting invasive behavior. In congenital disorders of glycosylation irregularities in glycan structures reflect defects in early developmental processes leading to multisystemic symptoms. These conditions emphasize the importance of B3GNT in maintaining normal cellular functions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GNT antibody (ab238818)
Paraffin-embedded human kidney tissue stained for B3GNT using ab238818 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GNT antibody (ab238818)
Paraffin-embedded human brain tissue stained for B3GNT using ab238818 at 1/100 dilution in immunohistochemical analysis.
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