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AB238818

Anti-B3GNT antibody

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(1 Publication)

Rabbit Polyclonal B3GNT antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human B4GAT1 aa 150 to C-terminus.

View Alternative Names

B3GNT1, B3GNT6, B4GAT1, Poly-N-acetyllactosamine extension enzyme, iGnT

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GNT antibody (AB238818)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GNT antibody (AB238818)

Paraffin-embedded human brain tissue stained for B3GNT using ab238818 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GNT antibody (AB238818)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GNT antibody (AB238818)

Paraffin-embedded human kidney tissue stained for B3GNT using ab238818 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human B4GAT1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43505

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

B3GNT also known as Beta-13-N-acetylglucosaminyltransferase is an enzyme that facilitates the transfer of N-acetylglucosamine from UDP-GlcNAc to various substrates. It often involves the synthesis of poly-N-acetyllactosamine chains. B3GNT family proteins weigh around 45 kDa although variations might exist. This enzyme mostly expresses in tissues such as the liver spleen and lungs. During mechanical processes B3GNT operates in the Golgi apparatus and actively participates in glycosylation processes.
Biological function summary

B3GNT enzymes play an important role in creating and modifying glycan structures on glycoproteins and glycolipids. Complex assemblies form with other glycosyltransferases for the efficient construction and regulation of glycan chains. These glycan structures are important for cellular processes like cell adhesion migration and signal transduction. By shaping these vital cellular events B3GNT contributes to fundamental biological functions and cell interactions.

Pathways

B3GNT performs essential functions in the glycosphingolipid biosynthesis and Notch signaling pathways. These pathways govern cell fate determination morphogenesis and differentiation. B3GNT interacts with various proteins including fucosyltransferases and sialyltransferases during the synthesis of complex glycan structures. The Notch signaling pathway also involves proteins such as Notch receptors and ligands where B3GNT influences receptor-ligand interactions through glycosylation modifications impacting the signaling outcomes.

B3GNT dysfunction correlates with conditions like cancer and congenital disorders of glycosylation. Changes in glycan structures due to altered B3GNT activity often link with tumorigenesis and cancer metastasis. B3GNT's relationship with proteins like E-cadherin in cancer can affect E-cadherin-mediated cell adhesion promoting invasive behavior. In congenital disorders of glycosylation irregularities in glycan structures reflect defects in early developmental processes leading to multisystemic symptoms. These conditions emphasize the importance of B3GNT in maintaining normal cellular functions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Beta-1,4-glucuronyltransferase involved in O-mannosylation of alpha-dystroglycan (DAG1) (PubMed : 19587235, PubMed : 23359570, PubMed : 25279697, PubMed : 25279699). Transfers a glucuronic acid (GlcA) residue onto a xylose (Xyl) acceptor to produce the glucuronyl-beta-1,4-xylose-beta disaccharide primer, which is further elongated by LARGE1, during synthesis of phosphorylated O-mannosyl glycan (PubMed : 25279697, PubMed : 25279699). Phosphorylated O-mannosyl glycan is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (PubMed : 25279697, PubMed : 25279699). Required for axon guidance; via its function in O-mannosylation of alpha-dystroglycan (DAG1) (By similarity).
See full target information B4GAT1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 2:100166 PubMed33521697

2021

A distinct innate immune signature marks progression from mild to severe COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Stéphane Chevrier,Yves Zurbuchen,Carlo Cervia,Sarah Adamo,Miro E Raeber,Natalie de Souza,Sujana Sivapatham,Andrea Jacobs,Esther Bachli,Alain Rudiger,Melina Stüssi-Helbling,Lars C Huber,Dominik J Schaer,Jakob Nilsson,Onur Boyman,Bernd Bodenmiller
View all publications
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