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AB96267

Anti-B3GNT3 antibody

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(2 Publications)

Rabbit Polyclonal B3GNT3 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human B3GNT3 aa 100-350.

View Alternative Names

B3GALT8, TMEM3, UNQ637/PRO1266, B3GNT3, Beta-3-Gx-T8, Core1-beta3GlcNAcT, Transmembrane protein 3, Beta3Gal-T8, Beta3GalT8, b3Gal-T8, BGnT-3, Beta3Gn-T3

1 Images
Western blot - Anti-B3GNT3 antibody (AB96267)
  • WB

Unknown

Western blot - Anti-B3GNT3 antibody (AB96267)

10% SDS-PAGE

All lanes:

Western blot - Anti-B3GNT3 antibody (ab96267) at 1/1000 dilution

All lanes:

293T whole cell lysate at 30 µg

Predicted band size: 43 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human B3GNT3 aa 100-350. The exact immunogen used to generate this antibody is proprietary information.

Q9Y2A9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

B3GNT3 also known as beta-13-N-acetylglucosaminyltransferase 3 plays a role in the synthesis of poly-N-acetyllactosamine chains. The target protein with a mass of approximately 40 kDa transfers N-acetylglucosamine from UDP-GlcNAc to a beta-galactoside link forming beta-13 linkage. B3GNT3 is important for the assembly of carbohydrate structures on cell surfaces. It is widely expressed in human tissues such as the lungs stomach and various epithelial cells where glycosylation is essential for cellular interaction and communication.
Biological function summary

B3GNT3 functions in the modification of glycoproteins and glycolipids affecting cellular adhesion and signaling processes. As a part of the larger glycosylation enzyme complex this enzyme influences the maturation of proteins through the addition of carbohydrate chains. The expression levels of B3GNT3 directly impact the stability and function of glycoconjugates which play roles in immune responses and cell-cell recognition.

Pathways

Cellular glycosylation processes heavily depend on B3GNT3 activity within the biosynthesis of poly-N-acetyllactosamine structures. This protein operates in the glycan metabolism pathway influencing the structure of glycans on glycoproteins and glycolipids. Through its interactions with other glycosyltransferases B3GNT3 affects cell signaling pathways including immune cell regulation and inflammation responses. The protein collaborates closely with other enzymes like B3GNT2 and B4GALT1 which also participate in glycan synthesis maintaining cellular homeostasis.

Disruptions in B3GNT3 activity lead to abnormalities in glycosylation patterns which relate to disorders such as cancer metastasis and congenital disorders of glycosylation. Altered B3GNT3 function affects cell adhesion properties contributing to cancer cell spread. Additionally it connects to immunological disorders through its influence on glycoprotein structures impacting immune recognition and response. B3GNT3's dysregulation pairs with proteins such as CD44 in cancer influencing the metastatic potential of tumor cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Has activity for type 2 oligosaccharides (PubMed : 11042166). Also acts as a core1-1,3-N-acetylglucosaminyltransferase (Core1-beta3GlcNAcT) to form the 6-sulfo sialyl Lewis x on extended core1 O-glycans (PubMed : 11439191).
See full target information B3GNT3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Thoracic cancer 14:1606-1617 PubMed37105940

2023

Propofol mediates non-small cell lung cancer growth in part by regulating circ_0003028-related mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuli Zhang,Dongzhi Liu,Ping Wang,Naihe Liu

Cancer management and research 12:2381-2391 PubMed32280275

2020

B3GNT3, a Direct Target of miR-149-5p, Promotes Lung Cancer Development and Indicates Poor Prognosis of Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Sun,Tao Liu,Lei Xian,Wenzhou Liu,Jun Liu,Huafu Zhou
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Product promise

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