Rabbit Polyclonal B3GNT3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human B3GNT3 aa 100-350.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human B3GNT3 aa 100-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y2A9
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Associated Products
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Target data
Function
Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Has activity for type 2 oligosaccharides (PubMed:11042166). Also acts as a core1-1,3-N-acetylglucosaminyltransferase (Core1-beta3GlcNAcT) to form the 6-sulfo sialyl Lewis x on extended core1 O-glycans (PubMed:11439191).
Alternative names
B3GALT8, TMEM3, UNQ637/PRO1266, B3GNT3, Beta-3-Gx-T8, Core1-beta3GlcNAcT, Transmembrane protein 3, Beta3Gal-T8, Beta3GalT8, b3Gal-T8, BGnT-3, Beta3Gn-T3
Recommended products
Rabbit Polyclonal B3GNT3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human B3GNT3 aa 100-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human B3GNT3 aa 100-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y2A9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
B3GNT3 also known as beta-13-N-acetylglucosaminyltransferase 3 plays a role in the synthesis of poly-N-acetyllactosamine chains. The target protein with a mass of approximately 40 kDa transfers N-acetylglucosamine from UDP-GlcNAc to a beta-galactoside link forming beta-13 linkage. B3GNT3 is important for the assembly of carbohydrate structures on cell surfaces. It is widely expressed in human tissues such as the lungs stomach and various epithelial cells where glycosylation is essential for cellular interaction and communication.
- Biological function summary
B3GNT3 functions in the modification of glycoproteins and glycolipids affecting cellular adhesion and signaling processes. As a part of the larger glycosylation enzyme complex this enzyme influences the maturation of proteins through the addition of carbohydrate chains. The expression levels of B3GNT3 directly impact the stability and function of glycoconjugates which play roles in immune responses and cell-cell recognition.
- Pathways
Cellular glycosylation processes heavily depend on B3GNT3 activity within the biosynthesis of poly-N-acetyllactosamine structures. This protein operates in the glycan metabolism pathway influencing the structure of glycans on glycoproteins and glycolipids. Through its interactions with other glycosyltransferases B3GNT3 affects cell signaling pathways including immune cell regulation and inflammation responses. The protein collaborates closely with other enzymes like B3GNT2 and B4GALT1 which also participate in glycan synthesis maintaining cellular homeostasis.
- Associated diseases and disorders
Disruptions in B3GNT3 activity lead to abnormalities in glycosylation patterns which relate to disorders such as cancer metastasis and congenital disorders of glycosylation. Altered B3GNT3 function affects cell adhesion properties contributing to cancer cell spread. Additionally it connects to immunological disorders through its influence on glycoprotein structures impacting immune recognition and response. B3GNT3's dysregulation pairs with proteins such as CD44 in cancer influencing the metastatic potential of tumor cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-B3GNT3 antibody (ab96267)
10% SDS-PAGE
All lanes: Western blot - Anti-B3GNT3 antibody (ab96267) at 1/1000 dilution
All lanes: 293T whole cell lysate at 30 µg
Predicted band size: 43 kDa
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