Rabbit Polyclonal B3GNT7 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human B3GNT7 aa 250-300 conjugated to Keyhole Limpet Haemocyanin.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human B3GNT7 aa 250-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NFL0
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
N-acetyl glucosamine (GlcNAc) transferase that catalyzes the transfer of GlcNAc via a beta1->3 linkage from UDP-GlcNAc to the non-reducing terminal galactose (Gal) in the linearly growing chain of N- and O-linked keratan sulfate proteoglycans. Cooperates with B4GALT4 galactosyltransferase and CHST6 and CHST1 sulfotransferases to construct and elongate mono- and disulfated disaccharide units [->3Galbeta1->4(6-sulfoGlcNAcbeta)1->] and [->3(6-sulfoGalbeta)1->4(6-sulfoGlcNAcbeta)1->] within keratan sulfate polymer (PubMed:14706853, PubMed:17690104). Involved in biosynthesis of N-linked keratan sulfate proteoglycans in cornea, with an impact on proteoglycan fibril organization and corneal transparency (By similarity) (PubMed:17690104). May play a role in the maintenance of tissue architecture by suppressing cellular motility and invasion (By similarity).
Alternative names
BGnT-7, Beta3Gn-T7, B3GNT7
Recommended products
Rabbit Polyclonal B3GNT7 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human B3GNT7 aa 250-300 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human B3GNT7 aa 250-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NFL0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
B3GNT7 also known as β-13-N-acetylglucosaminyltransferase 7 is an enzyme involved in glycosylation processes. It has a mass of approximately 46 kDa. Found within the Golgi apparatus of cells B3GNT7 is responsible for catalyzing the transfer of N-acetylglucosamine (GlcNAc) residues to precursor oligosaccharides forming β1-3 glycosidic bonds. It shows widespread expression but levels are higher in tissues such as brain pancreas and kidneys indicating tissue-specific roles.
- Biological function summary
B3GNT7 plays an important role in the biosynthesis of glycosaminoglycans which are complex polysaccharides. These polysaccharides provide structure and support to cells and tissues partaking in processes like cell signaling and maintaining cellular environment. B3GNT7 is not part of multienzyme complexes but interacts closely with other glycosylation enzymes. This specificity influences the structural diversity and function of glycosaminoglycans in various biological processes.
- Pathways
B3GNT7 participates in the glycosaminoglycan biosynthesis pathway particularly in the synthesis of keratan sulfate. This pathway impacts cellular communication and structural integrity significant for tissue function. B3GNT7 functions alongside related enzymes such as B3GNT1 which also contribute to glycosaminoglycan chain elongation. The interactions with these enzymes play an important role in modifying glycosaminoglycan chains for distinct biological functions.
- Associated diseases and disorders
Alterations in B3GNT7 expression and function relate to osteoarthritis and certain neuropathies. In osteoarthritis abnormal glycosaminoglycan synthesis can lead to impaired joint function and cartilage deterioration. Interactions with proteins like B3GNT6 which has overlapping roles in glycosylation may influence disease progression. Understanding B3GNT7's impact on these conditions helps in developing targeted therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B3GNT7 antibody (ab190217)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling B3GNT7 with ab190217 at 1/50 dilution.
Western blot - Anti-B3GNT7 antibody (ab190217)
All lanes: Western blot - Anti-B3GNT7 antibody (ab190217) at 1/1000 dilution
All lanes: Mouse heart lysate at 35 µg
Predicted band size: 46 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com