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AB200639

Anti-b4Gal-T6 antibody

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(1 Publication)

Rabbit Polyclonal b4Gal-T6 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human B4GALT6 aa 300 to C-terminus.

View Alternative Names

Beta4Gal-T6, b4Gal-T6, Lactosylceramide synthase, LacCer synthase, B4GALT6

1 Images
Western blot - Anti-b4Gal-T6 antibody (AB200639)
  • WB

Supplier Data

Western blot - Anti-b4Gal-T6 antibody (AB200639)

All lanes:

Western blot - Anti-b4Gal-T6 antibody (ab200639)

Lane 1:

HEK293T whole cell lysate

Lane 2:

A549 whole cell lysate

Lane 3:

mouse sp/0 whole cell lysate

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human B4GALT6 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UBX8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab200639 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Storage buffer
pH: 7.2 Preservative: 0.097% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

B4Gal-T6 also known as beta-14-galactosyltransferase 6 is an enzyme that catalyzes the transfer of galactose to specific acceptor molecules like N-acetylglucosamine. It is approximately 46 kDa in size. It plays a role in the biosynthesis of glycoproteins and glycolipids. This enzyme is expressed in several tissues including the liver kidney and brain. Its activity contributes to the formation of complex carbohydrates that occur on cell surfaces and in secreted proteins.
Biological function summary

B4Gal-T6 functions in the assembly of glycosaminoglycans (GAGs) which form the carbohydrate components of the extracellular matrix. This enzyme acts as part of a larger complex involving other glycosyltransferases that regulate the elongation of polysaccharide chains. It ensures the proper structure and function of proteoglycans in tissues affecting cell adhesion migration and proliferation.

Pathways

B4Gal-T6 is involved in the glycosylation pathways related to the synthesis of GAGs and the N-linked glycosylation of proteins. It interacts with other enzymes like b4Gal-T1 within these pathways ensuring the correct formation and modification of glycan structures. The enzyme contributes to pathways such as the Wnt signaling pathway where proper glycosylation is necessary for pathway activation and function.

Abnormal b4Gal-T6 activity can associate with cancer and Ehlers-Danlos syndrome. In cancer altered glycosylation patterns can influence tumor growth and metastasis. The enzyme interacts with proteins such as decorin which is involved in collagen fibril organization and skin integrity linking its dysfunction to Ehlers-Danlos syndrome. Understanding its role could provide targets for therapeutic intervention in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the synthesis of lactosylceramide (LacCer) via the transfer of galactose from UDP-galactose to glucosylceramide (GlcCer) (PubMed : 1551920, PubMed : 24498430, PubMed : 3099851). LacCer is the starting point in the biosynthesis of all gangliosides (membrane-bound glycosphingolipids) which play pivotal roles in the CNS including neuronal maturation and axonal and myelin formation (By similarity).
See full target information B4GALT6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 7:eabh2148 PubMed34739319

2021

PD-1 independent of PD-L1 ligation promotes glioblastoma growth through the NFκB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Reza Mirzaei,Ashley Gordon,Franz J Zemp,Mehul Kumar,Susobhan Sarkar,H Artee Luchman,Anita C Bellail,Chunhai Hao,Douglas J Mahoney,Jeff F Dunn,Pinaki Bose,V Wee Yong
View all publications
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Product promise

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