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Rabbit Recombinant Monoclonal B4GALNT1 antibody. Suitable for WB, IHC-P, Dot and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] (AB314130), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] (AB314130), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] (AB314130), expandable thumbnail
  • Western blot - Anti-B4GALNT1 antibody [EPR28064-64] (AB314130), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] (AB314130), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PDotICC/IFFlow Cyt (Intra)IP
Human
Tested
Tested
Expected
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Expected
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Expected
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human, Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Involved in the biosynthesis of gangliosides GM2, GD2, GT2 and GA2 from GM3, GD3, GT3 and GA3, respectively.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal B4GALNT1 antibody. Suitable for WB, IHC-P, Dot and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR28064-64

Purification technique

Affinity purification Protein A

Specificity

Unsuitable for mouse IHC-P, rat IHC-P

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

B4GALNT1 also known as beta-14-N-acetylgalactosaminyltransferase 1 is an enzyme that plays a role in the biosynthesis of gangliosides. It has a molecular mass of approximately 56 kDa. B4GALNT1 catalyzes the transfer of N-acetylgalactosamine to substrates in the Golgi apparatus which facilitates the formation of gangliosides. This enzyme exhibits expression mainly in the central nervous system and several other tissues reflecting its role in the development and function of neural cells.

Biological function summary

The enzyme contributes to the synthesis of complex gangliosides. B4GALNT1 does not work alone; instead it integrates into a system of glycosylation pathways involving other glycosyltransferases forming a complex network that tailors the composition of glycan structures on lipids and proteins. This activity influences cell recognition adhesion and signaling processes important for neurodevelopment and synapse formation.

Pathways

B4GALNT1 influences the ganglioside biosynthetic pathway an important aspect for proper neuronal function. This pathway involves collaboration with other proteins such as GM2 synthase which cooperates in the addition of sugar residues at various stages of glycolipid formation. These interactions ensure the synthesis of diverse ganglioside molecules within nerve cell membranes which are vital in modulating cellular interactions and signal transduction.

Associated diseases and disorders

Alterations in B4GALNT1 function have connections to neurological disorders such as Alzheimer's disease and certain types of gangliosidosis. Perturbations in its enzymatic activity can disrupt normal ganglioside composition affecting membrane dynamics and cell signaling. Phenomena linked to other glycosylation enzymes like GM3 synthase also illustrate disease pathways impacted by aberrant glycan synthesis highlighting B4GALNT1's indirect influence on these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling B4GALNT1 with ab314130 at 1/500 (1.02 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression: no staining on human skeletal muscle. The section was incubated with ab314130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130)

    Immunohistochemical analysis of paraffin-embedded human lung tissue labeling B4GALNT1 with ab314130 at 1/500 (1.02 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human lung (PMID: 33367717). The section was incubated with ab314130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control:Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130)

    Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labeling B4GALNT1 with ab314130 at 1/500 (1.02 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human astrocytoma. The section was incubated with ab314130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130), expandable thumbnail

    Western blot - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8824296, 35356430, 22638855).
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
    Exposure time: 37 seconds

    All lanes: Western blot - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130) at 1/1000 dilution

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 2: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 75 kDa, 80 kDa

    Exposure time: 37s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling B4GALNT1 with ab314130 at 1/500 (1.02 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with ab314130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130), expandable thumbnail

    Western blot - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: skeletal muscle (PMID: 28709807).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8824296, 35356430, 22638855).
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
    Exposure time: 180 seconds

    All lanes: Western blot - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130) at 1/1000 dilution

    Lane 1: Mouse cerebellum tissue lysate at 20 µg

    Lane 2: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 3: Rat cerebellum tissue lysate at 20 µg

    Lane 4: Rat skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 75 kDa, 80 kDa

    Exposure time: 180s

  • Western blot - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130), expandable thumbnail

    Western blot - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: skeletal muscle (PMID: 28709807).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8824296, 35356430, 22638855).
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
    Exposure time: 180 seconds

    All lanes: Western blot - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 20 µg

    Lane 2: Human skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 80 kDa

    Exposure time: 180s

  • Western blot - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130), expandable thumbnail

    Western blot - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: 293T (PMID: 28709807).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8824296, 35356430, 22638855).
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
    Exposure time: 37 seconds

    All lanes: Western blot - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130) at 1/1000 dilution

    Lane 1: IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 2: SK-N-SH (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 4: A549 (human lu carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 5: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 75 kDa, 80 kDa

    Exposure time: 37s

  • Dot Blot - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130), expandable thumbnail

    Dot Blot - Anti-B4GALNT1 antibody [EPR28064-64] (ab314130)

    Dot blot analysis of B4GALNT1 using ab314130 at 1:1000 (0.51 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
    Lane 1: Human B4GALNT1 fragment
    Lane 2: Human B4GALNT2 fragment
    Exposure time: 180 seconds
    Blocking and diluting buffer and concentration: 5% NFDM/TBST

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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