AB314131

Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal B4GALNT1 antibody. Carrier free. Suitable for WB, IHC-P, Dot and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

View Alternative Names

GALGT, SIAT2, B4GALNT1, (N-acetylneuraminyl)-galactosylglucosylceramide, GM2/GD2 synthase, GalNAc-T

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

This data was developed using ab314130, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling B4GALNT1 with ab314130 at 1/500 (1.02 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with ab314130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

This data was developed using ab314130, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling B4GALNT1 with ab314130 at 1/500 (1.02 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression : no staining on human skeletal muscle. The section was incubated with ab314130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

This data was developed using ab314130, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human lung tissue labeling B4GALNT1 with ab314130 at 1/500 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human lung (PMID : 33367717). The section was incubated with ab314130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

This data was developed using ab314130, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling B4GALNT1 with ab314130 at 1/500 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human astrocytoma. The section was incubated with ab314130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Supplier Data

Western blot - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

This data was developed using ab314130, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : 293T (PMID : 28709807). The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8824296, 35356430, 22638855). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : 37 seconds

All lanes:

Western blot - Anti-B4GALNT1 antibody [EPR28064-64] (<a href='/en-us/products/primary-antibodies/b4galnt1-antibody-epr28064-64-ab314130'>ab314130</a>) at 1/1000 dilution

Lane 1:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 2:

SK-N-SH (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 4:

A549 (human lu carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 5:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,80 kDa

false

Exposure time: 37s

Supplier Data

Western blot - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

This data was developed using ab314130, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : skeletal muscle (PMID : 28709807). The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8824296, 35356430, 22638855). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-B4GALNT1 antibody [EPR28064-64] (<a href='/en-us/products/primary-antibodies/b4galnt1-antibody-epr28064-64-ab314130'>ab314130</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa

false

Exposure time: 180s

Supplier Data

Dot Blot - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

This data was developed using ab314130, the same antibody clone in a different buffer formulation. Dot blot analysis of B4GALNT1 using ab314130 at 1 : 1000 (0.51 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Lane 1 : Human B4GALNT1 fragment Lane 2 : Human B4GALNT2 fragment Exposure time : 180 seconds Blocking and diluting buffer and concentration : 5% NFDM/TBST

Supplier Data

Western blot - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

This data was developed using ab314130, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8824296, 35356430, 22638855). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : 37 seconds

All lanes:

Western blot - Anti-B4GALNT1 antibody [EPR28064-64] (<a href='/en-us/products/primary-antibodies/b4galnt1-antibody-epr28064-64-ab314130'>ab314130</a>) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 2:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,80 kDa

false

Exposure time: 37s

Supplier Data

Western blot - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

This data was developed using ab314130, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : skeletal muscle (PMID : 28709807). The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8824296, 35356430, 22638855). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-B4GALNT1 antibody [EPR28064-64] (<a href='/en-us/products/primary-antibodies/b4galnt1-antibody-epr28064-64-ab314130'>ab314130</a>) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 3:

Rat cerebellum tissue lysate at 20 µg

Lane 4:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,80 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28064-64

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB, Dot, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Unsuitable for mouse IHC-P, rat IHC-P

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Reactivity data

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Product details

ab314131 is the carrier-free version of ab314130.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

B4GALNT1 also known as beta-14-N-acetylgalactosaminyltransferase 1 is an enzyme that plays a role in the biosynthesis of gangliosides. It has a molecular mass of approximately 56 kDa. B4GALNT1 catalyzes the transfer of N-acetylgalactosamine to substrates in the Golgi apparatus which facilitates the formation of gangliosides. This enzyme exhibits expression mainly in the central nervous system and several other tissues reflecting its role in the development and function of neural cells.

Biological function summary

The enzyme contributes to the synthesis of complex gangliosides. B4GALNT1 does not work alone; instead it integrates into a system of glycosylation pathways involving other glycosyltransferases forming a complex network that tailors the composition of glycan structures on lipids and proteins. This activity influences cell recognition adhesion and signaling processes important for neurodevelopment and synapse formation.

Pathways

B4GALNT1 influences the ganglioside biosynthetic pathway an important aspect for proper neuronal function. This pathway involves collaboration with other proteins such as GM2 synthase which cooperates in the addition of sugar residues at various stages of glycolipid formation. These interactions ensure the synthesis of diverse ganglioside molecules within nerve cell membranes which are vital in modulating cellular interactions and signal transduction.

Alterations in B4GALNT1 function have connections to neurological disorders such as Alzheimer's disease and certain types of gangliosidosis. Perturbations in its enzymatic activity can disrupt normal ganglioside composition affecting membrane dynamics and cell signaling. Phenomena linked to other glycosylation enzymes like GM3 synthase also illustrate disease pathways impacted by aberrant glycan synthesis highlighting B4GALNT1's indirect influence on these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the biosynthesis of gangliosides GM2, GD2, GT2 and GA2 from GM3, GD3, GT3 and GA3, respectively.

See full target information B4GALNT1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

Western blot - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

Western blot - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

Dot Blot - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

Western blot - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

Western blot - Anti-B4GALNT1 antibody [EPR28064-64] - BSA and Azide free (AB314131)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise