AB121326

Anti-B4GALT1 antibody

Name: Anti-B4GALT1 antibody (ab121326) | Abcam
Brand: Abcam Limited
SKU: AB121326
Price: 435 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(10 Publications)

Rabbit Polyclonal B4GALT1 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human B4GALT1 aa 50-200.

View Alternative Names

GGTB2, B4GALT1, Beta4Gal-T1, b4Gal-T1, Lactose synthase A protein, N-acetyllactosamine synthase, Nal synthase

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT1 antibody (AB121326)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT1 antibody (AB121326)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling B4GALT1 with ab121326 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT1 antibody (AB121326)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling B4GALT1 with ab121326 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT1 antibody (AB121326)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling B4GALT1 with ab121326 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT1 antibody (AB121326)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling B4GALT1 with ab121326 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-B4GALT1 antibody (AB121326)

Loading control : Anti-HSP90B1

All lanes:

Western blot - Anti-B4GALT1 antibody (ab121326) at 2 µg

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 2:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 44 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC

applications

Immunogen

Recombinant Fragment Protein within Human B4GALT1 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

P15291

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

B4GALT1 also known as beta-14-galactosyltransferase 1 is an enzyme that facilitates the transfer of galactose residues from UDP-galactose to acceptor sugars forming β1-4 galactosidic linkages. This enzyme has an approximate mass of 60 kDa. It is primarily expressed in the Golgi apparatus of many cell types including epithelial cells and fibroblasts. B4GALT1 represents one of seven beta-14-galactosyltransferase enzymes each with varying specificity and function depending on the tissue type.

Biological function summary

B4GALT1 plays an important role in the modification of glycoproteins and glycolipids. It acts as part of a larger complex responsible for the formation and maintenance of glycan structures on cellular proteins which are important for cell-cell communication and protein stability. This modification process is essential for normal cellular functions because it impacts protein folding secretion and cell surface expression. B4GALT1 achieves this by participating in the synthesis of N-linked and O-linked glycans which directly influences various physiological processes.

Pathways

B4GALT1 functions in the glycosylation pathway where it modifies oligosaccharide chains during protein post-translational modification. This pathway is essential for the synthesis of glycoproteins which play a role in cell adhesion and signaling. B4GALT1 also contributes to the protein N-glycosylation metabolic pathway and interacts with proteins like ST3GAL1 which modify glycan structures further. These pathways are indispensable for proper cellular communication and function.

Defects or dysregulation of B4GALT1 can lead to conditions such as congenital disorders of glycosylation. Mutations in this enzyme have been linked to neurological and developmental abnormalities due to impaired glycan synthesis. B4GALT1 has also shown relevance in cancer influencing tumor progression and metastasis. It interacts with MUC1 a protein that when aberrantly glycosylated can promote cancer cell invasiveness and impede immune response therefore affecting cancer prognosis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Galactosyltransferase acting in the Golgi stacks. Catalyzes the transfer of galactose (Gal) from UDP-alpha-D-galactose in beta(1->4) linkage to the non-reducing terminal N-acetylglucosamine (GlcNAc) moieties of glycolipids and complex-type N-linked glycans (PubMed : 16157350, PubMed : 27872474, PubMed : 29133956, PubMed : 36280670, PubMed : 37632720, PubMed : 38321209). Adds one Gal residue to both GlcNAc beta(1->2)-linked to the alpha(1->3) and alpha(1->6) mannose antennae of complex-type N-glycans, enabling the formation of mono- and di-galactosylated glycoforms. Galactosylates complex-type N-glycans attached on the fragment crystallizable (Fc) of immunoglobulin-gamma isotypes (IgGs), a prerequisite for antibody glycan sialylation and related anti-inflammatory effector functions (PubMed : 27872474, PubMed : 29133956, PubMed : 36280670, PubMed : 37632720). Can also transfer a Gal residue to free GlcNAc to form N-acetyllactosamine (PubMed : 16157350). With LALBA/alpha-lactalbumin forms the lactose synthase complex responsible for production of large amounts of lactose in the lactating mammary gland. Interaction with LALBA alters the sugar substrate specificity of the catalytic domain, enabling high affinity binding of glucose and its transformation to lactose (PubMed : 16157350).. Isoform Long. The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, like those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix. Acts as a sperm receptor for ZP3 O-linked glycans on the zona pellucida leading to activation of G-protein signaling and acrosome reaction.. Processed beta-1,4-galactosyltransferase 1. The secreted form is proficient in galactosyltransferase activity and could be involved in glycan remodeling in biological fluids.

See full target information B4GALT1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 32:1907-1919 PubMed40629165

2025

Glycerol mediates crosstalk between metabolism and trafficking through the golgin Imh1.

Applications

Unspecified application

Species

Unspecified reactive species

Wan-Yun Chiu,Yi-Hsun Wang,Ming-Chieh Lin,Chun-Chi Lai,Chia-Jung Yu,Fang-Jen S Lee

Journal of experimental & clinical cancer research : CR 42:146 PubMed37303063

2023

B4GALT1 promotes immune escape by regulating the expression of PD-L1 at multiple levels in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Cui,Jun Li,Pengpeng Zhang,Dandan Yin,Ziyu Wang,Jiali Dai,Wei Wang,Erbao Zhang,Renhua Guo

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2204786 PubMed36504438

2022

Single-Cell and Spatial Transcriptomics Decodes Wharton's Jelly-Derived Mesenchymal Stem Cells Heterogeneity and a Subpopulation with Wound Repair Signatures.

Applications

Unspecified application

Species

Unspecified reactive species

Penghong Chen,Shijie Tang,Ming Li,Dezhi Wang,Caixiang Chen,Yiqun Qiu,Zhuoqun Fang,Haoruo Zhang,Hangqi Gao,Haiyan Weng,Kailun Hu,Jian Lin,Qingxia Lin,Yi Tan,Shirong Li,Jinghua Chen,Liangwan Chen,Xiaosong Chen

Frontiers in genetics 13:882004 PubMed36568388

2022

High expression of B4GALT1 is associated with poor prognosis in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihong Ren,Xiaoyu Huang,Qing Lv,Yiming Lei,Haiqiang Shi,Fanping Wang,Mingyong Wang

International journal of molecular sciences 23: PubMed36499368

2022

B4GALT1 as a New Biomarker of Idiopathic Pulmonary Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia De Vitis,Michela D'Ascanio,Andrea Sacconi,Dario Pizzirusso,Valentina Salvati,Massimiliano Mancini,Giorgia Scafetta,Roberto Cirombella,Francesca Ascenzi,Sara Bruschini,Antonella Esposito,Silvia Castelli,Claudia Salvucci,Leonardo Teodonio,Bruno Sposato,Angela Catizone,Arianna Di Napoli,Andrea Vecchione,Gennaro Ciliberto,Salvatore Sciacchitano,Alberto Ricci,Rita Mancini

Cancer letters 500:228-243 PubMed33309857

2020

Galactosyltransferase B4GALT1 confers chemoresistance in pancreatic ductal adenocarcinomas by upregulating N-linked glycosylation of CDK11.

Applications

Unspecified application

Species

Unspecified reactive species

Yitian Chen,Liangping Su,Cheng Huang,Sangqing Wu,Xiaoyi Qiu,Xinbao Zhao,Qiong Meng,Ya-Ming Meng,Xiangzhan Kong,Minghui Wang,Chao Liu,Ping-Pui Wong

Technology in cancer research & treatment 19:1533033820980104 PubMed33287670

2020

B4GalT1 Regulates Apoptosis and Autophagy of Glioblastoma In Vitro and In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Pu Wang,Xiaolong Li,Yuan Xie

Molecular cancer 19:147 PubMed33032611

2020

Identification of a distinct luminal subgroup diagnosing and stratifying early stage prostate cancer by tissue-based single-cell RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoshi Ma,Jinan Guo,Kaisheng Liu,Lipeng Chen,Dale Liu,Shaowei Dong,Jinquan Xia,Qiaoyun Long,Yongjian Yue,Pan Zhao,Fengyan Hu,Zhangang Xiao,Xinghua Pan,Kefeng Xiao,Zhiqiang Cheng,Zunfu Ke,Zhe-Sheng Chen,Chang Zou

American journal of physiology. Renal physiology 319:F312-F322 PubMed32628542

2020

Upregulated proteoglycan-related signaling pathways in fluid flow shear stress-treated podocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Tarak Srivastava,Trupti Joshi,Yuexu Jiang,Daniel P Heruth,Mohamed H Rezaiekhaligh,Jan Novak,Vincent S Staggs,Uri S Alon,Robert E Garola,Ashraf El-Meanawy,Ellen T McCarthy,Jianping Zhou,Varun C Boinpelly,Ram Sharma,Virginia J Savin,Mukut Sharma

Acta biochimica et biophysica Sinica 48:1120-1127 PubMed27797721

2016

ZFX modulates the growth of human leukemic cells via B4GALT1.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Wu,Lun Xiao,Haixia Zhou,Hong Liu,Yue Ge,Jing Yang,Yuanyuan Li,Depei Wu,Yun Zhao,Xiuyan Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-B4GALT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT1 antibody (AB121326)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT1 antibody (AB121326)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT1 antibody (AB121326)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT1 antibody (AB121326)

Western blot - Anti-B4GALT1 antibody (AB121326)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise