JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB155476

Anti-B4GALT4 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal B4GALT4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human B4GALT4 aa 100 to C-terminus.

View Alternative Names

UNQ552/PRO1109, B4GALT4, Beta4Gal-T4, b4Gal-T4, N-acetyllactosamine synthase, Nal synthase

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT4 antibody (AB155476)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT4 antibody (AB155476)

Immunohistochemical analysis of paraffin-embedded Human H441 xenograft tissue, labeling B4GALT4 using ab155476 at a 1/250 dilution.

Western blot - Anti-B4GALT4 antibody (AB155476)
  • WB

Unknown

Western blot - Anti-B4GALT4 antibody (AB155476)

10% SDS PAGE

All lanes:

Western blot - Anti-B4GALT4 antibody (ab155476) at 1/1000 dilution

All lanes:

Jurkat whole cell lysate at 30 µg

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human B4GALT4 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60513

style
left-column

Alternative Products

Primary Antibodies

AB169604

Anti-B4GALT4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-B4GALT4 antibody (AB169604)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB121326

Anti-B4GALT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B4GALT1 antibody (AB121326)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB192890

Anti-LC3B antibody [EPR18709] - Autophagosome Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB192890)

  • 5
  • 19
Primary Antibodies

AB176594

Anti-ZZEF1 antibody

Immunoprecipitation - Anti-ZZEF1 antibody (AB176594)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The B4GALT4 protein also known as beta-14-galactosyltransferase 4 participates in the addition of galactose to poly-N-acetyllactosaminoglycan structures found on glycans. This enzymatic activity occurs primarily in the Golgi apparatus where B4GALT4 modifies glycoproteins and glycolipids. It has a molecular mass of approximately 45 kDa. Expression of this protein happens across various tissues including the liver kidney and brain indicating its involvement in diverse biological processes.
Biological function summary

B4GALT4 helps in carbohydrate metabolism by acting as part of a complex. It plays an important role in regulating cell-cell recognition and signaling. Its activity affects the composition of glycoconjugates involved in immune responses and cellular interactions. The protein is essential in forming N-glycans which are critical components for extracellular matrix organization and function.

Pathways

B4GALT4 features prominently in the glycosylation pathway. It influences the biosynthesis of glycoproteins and other related molecules. In the context of glycosylation B4GALT4 works alongside other glycosyltransferases like B3GALT6 and contributes to essential pathways such as the galactose metabolism. These pathways are vital for generating complex glycans necessary for various cellular functions.

Aberrations in B4GALT4 activity associate with certain congenital disorders of glycosylation (CDG) and some cancers. In CDG mutations impacting this protein cause improper glycan structures leading to multisystemic disease symptoms. Cancers sometimes show altered expression or activity of glycosyltransferases including B4GALT4 affecting cell behavior and tumor progression. The protein’s interaction with components such as B3GALT6 in these conditions highlights its importance in maintaining cellular homeostasis and normal physiological function.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Galactose (Gal) transferase involved in the synthesis of terminal N-acetyllactosamine (LacNac) unit present on glycan chains of glycoproteins and glycosphingolipids (PubMed : 12511560, PubMed : 17690104, PubMed : 32827291, PubMed : 9792633). Catalyzes the transfer of Gal residue via a beta1->4 linkage from UDP-Gal to the non-reducing terminal N-acetyl glucosamine 6-O-sulfate (6-O-sulfoGlcNAc) in the linearly growing chain of both N- and O-linked keratan sulfate proteoglycans. Cooperates with B3GNT7 N-acetyl glucosamine transferase and CHST6 and CHST1 sulfotransferases to construct and elongate mono- and disulfated disaccharide units [->3Galbeta1->4(6-sulfoGlcNAcbeta)1->] and [->3(6-sulfoGalbeta)1->4(6-sulfoGlcNAcbeta)1->] within keratan sulfate polymer (PubMed : 17690104). Transfers Gal residue via a beta1->4 linkage to terminal 6-O-sulfoGlcNAc within the LacNac unit of core 2 O-glycans forming 6-sulfo-sialyl-Lewis X (sLex). May contribute to the generation of sLex epitope on mucin-type glycoproteins that serve as ligands for SELL/L-selectin, a major regulator of leukocyte migration (PubMed : 12511560). In the biosynthesis pathway of neolacto-series glycosphingolipids, transfers Gal residue via a beta1->4 linkage to terminal GlcNAc of a lactotriaosylceramide (Lc3Cer) acceptor to form a neolactotetraosylceramide (PubMed : 9792633). Efficiently galactosylates both non-sulfated and 6-O-sulfated terminal GlcNAc moities on G0 complex-type N-glycans.
See full target information B4GALT4
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 39:617-636 PubMed31527668

2019

Nuclear control of lung cancer cells migration, invasion and bioenergetics by eukaryotic translation initiation factor 3F.

Applications

Unspecified application

Species

Unspecified reactive species

Pauline Esteves,Laetitia Dard,Aurélia Brillac,Christophe Hubert,Saharnaz Sarlak,Benoît Rousseau,Elodie Dumon,Julien Izotte,Marc Bonneu,Didier Lacombe,Jean-William Dupuy,Nivea Amoedo,Rodrigue Rossignol
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com