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AB169604

Anti-B4GALT4 antibody

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(1 Publication)

Mouse Polyclonal B4GALT4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human B4GALT4.

View Alternative Names

UNQ552/PRO1109, B4GALT4, Beta4Gal-T4, b4Gal-T4, N-acetyllactosamine synthase, Nal synthase

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-B4GALT4 antibody (AB169604)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-B4GALT4 antibody (AB169604)

Immunofluorescent analysis of HeLa cells labeling B4GALT4 with ab169604 at 10 µg/ml.

Western blot - Anti-B4GALT4 antibody (AB169604)
  • WB

Unknown

Western blot - Anti-B4GALT4 antibody (AB169604)

All lanes:

Western blot - Anti-B4GALT4 antibody (ab169604) at 1 µg/mL

All lanes:

Human kidney tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 40 kDa

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Western blot - Anti-B4GALT4 antibody (AB169604)
  • WB

Unknown

Western blot - Anti-B4GALT4 antibody (AB169604)

All lanes:

Western blot - Anti-B4GALT4 antibody (ab169604) at 1 µg/mL

Lane 1:

B4GALT4 transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 40 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human B4GALT4.

O60513

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The B4GALT4 protein also known as beta-14-galactosyltransferase 4 participates in the addition of galactose to poly-N-acetyllactosaminoglycan structures found on glycans. This enzymatic activity occurs primarily in the Golgi apparatus where B4GALT4 modifies glycoproteins and glycolipids. It has a molecular mass of approximately 45 kDa. Expression of this protein happens across various tissues including the liver kidney and brain indicating its involvement in diverse biological processes.
Biological function summary

B4GALT4 helps in carbohydrate metabolism by acting as part of a complex. It plays an important role in regulating cell-cell recognition and signaling. Its activity affects the composition of glycoconjugates involved in immune responses and cellular interactions. The protein is essential in forming N-glycans which are critical components for extracellular matrix organization and function.

Pathways

B4GALT4 features prominently in the glycosylation pathway. It influences the biosynthesis of glycoproteins and other related molecules. In the context of glycosylation B4GALT4 works alongside other glycosyltransferases like B3GALT6 and contributes to essential pathways such as the galactose metabolism. These pathways are vital for generating complex glycans necessary for various cellular functions.

Aberrations in B4GALT4 activity associate with certain congenital disorders of glycosylation (CDG) and some cancers. In CDG mutations impacting this protein cause improper glycan structures leading to multisystemic disease symptoms. Cancers sometimes show altered expression or activity of glycosyltransferases including B4GALT4 affecting cell behavior and tumor progression. The protein’s interaction with components such as B3GALT6 in these conditions highlights its importance in maintaining cellular homeostasis and normal physiological function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Galactose (Gal) transferase involved in the synthesis of terminal N-acetyllactosamine (LacNac) unit present on glycan chains of glycoproteins and glycosphingolipids (PubMed : 12511560, PubMed : 17690104, PubMed : 32827291, PubMed : 9792633). Catalyzes the transfer of Gal residue via a beta1->4 linkage from UDP-Gal to the non-reducing terminal N-acetyl glucosamine 6-O-sulfate (6-O-sulfoGlcNAc) in the linearly growing chain of both N- and O-linked keratan sulfate proteoglycans. Cooperates with B3GNT7 N-acetyl glucosamine transferase and CHST6 and CHST1 sulfotransferases to construct and elongate mono- and disulfated disaccharide units [->3Galbeta1->4(6-sulfoGlcNAcbeta)1->] and [->3(6-sulfoGalbeta)1->4(6-sulfoGlcNAcbeta)1->] within keratan sulfate polymer (PubMed : 17690104). Transfers Gal residue via a beta1->4 linkage to terminal 6-O-sulfoGlcNAc within the LacNac unit of core 2 O-glycans forming 6-sulfo-sialyl-Lewis X (sLex). May contribute to the generation of sLex epitope on mucin-type glycoproteins that serve as ligands for SELL/L-selectin, a major regulator of leukocyte migration (PubMed : 12511560). In the biosynthesis pathway of neolacto-series glycosphingolipids, transfers Gal residue via a beta1->4 linkage to terminal GlcNAc of a lactotriaosylceramide (Lc3Cer) acceptor to form a neolactotetraosylceramide (PubMed : 9792633).
See full target information B4GALT4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Human molecular genetics : PubMed30986821

2019

The protective variant rs7173049 at LOXL1 locus impacts on retinoic acid signaling pathway in pseudoexfoliation syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Berner,Ursula Hoja,Matthias Zenkel,James Julian Ross,Steffen Uebe,Daniela Paoli,Paolo Frezzotti,Robyn M Rautenbach,Ari Ziskind,Susan E Williams,Trevor R Carmichael,Michele Ramsay,Fotis Topouzis,Anthi Chatzikyriakidou,Alexandros Lambropoulos,Periasamy Sundaresan,Humaira Ayub,Farah Akhtar,Raheel Qamar,Juan C Zenteno,Marisa Cruz-Aguilar,Yury S Astakhov,Michael Dubina,Janey Wiggs,Mineo Ozaki,Friedrich E Kruse,Tin Aung,André Reis,Chiea Chuen Khor,Francesca Pasutto,Ursula Schlötzer-Schrehardt
View all publications
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