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AB110398

Anti-B4GALT5 antibody

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(2 Publications)

Rabbit Polyclonal B4GALT5 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human B4GALT5.

View Alternative Names

Beta4Gal-T5, b4Gal-T5, Lactosylceramide synthase, LacCer synthase, B4GALT5

1 Images
Western blot - Anti-B4GALT5 antibody (AB110398)
  • WB

Unknown

Western blot - Anti-B4GALT5 antibody (AB110398)

All lanes:

Western blot - Anti-B4GALT5 antibody (ab110398) at 1/500 dilution

Lane 1:

Extracts from HeLa cells at 30 µg

Lane 2:

Extracts from 293 cells at 30 µg

Lane 3:

Extracts from Huvec cells at 30 µg

Lane 4:

Extracts from HuvEc cells at 30 µg with immunising peptide

Predicted band size: 45 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human B4GALT5. The exact immunogen used to generate this antibody is proprietary information.

O43286

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

B4GALT5 also known as beta-14-galactosyltransferase 5 is an enzyme critical for glycosylation processes. This enzyme has a molecular weight of approximately 43 kDa. It adds galactose to specific carbohydrate structures and plays a role in forming lactose and other glycan structures. This enzyme is part of the larger beta-14-galactosyltransferase family and is found in the Golgi apparatus. It is expressed in various tissues with significant activity in the liver and kidney and to a certain extent in the brain.
Biological function summary

B4GALT5 is involved in the synthesis of complex glycoconjugates and lactose. It contributes to the N-linked and O-linked glycosylation of proteins which are essential for protein folding stability and cell signaling. B4GALT5 forms part of the enzyme complex responsible for elongating glycan chains cooperating with other glycosyltransferases. Through its enzymatic activity it affects cell-cell interactions host-pathogen recognition and immune responses.

Pathways

B4GALT5 is an important component of the glycan biosynthesis and metabolism pathways. Its action affects the glycoprotein quality control and folding pathways. In these processes it works alongside other glycosyltransferases such as B4GALT1 ensuring proper glycoconjugate formation and modification. These pathways influence developmental processes immune system functions and normal cellular operations.

B4GALT5 has links to congenital disorders of glycosylation and cancer. Abnormal glycosylation can alter cell adhesion and migration contributing to tumorigenesis and metastasis. The enzyme shows altered expression levels in certain cancers suggesting its role as a potential biomarker. B4GALT5 has associations with other proteins like E-cadherin where changes in glycosylation patterns can influence cancer progression and immune evasion.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the synthesis of lactosylceramide (LacCer) via the transfer of galactose from UDP-galactose to glucosylceramide (GlcCer) (PubMed : 24498430). LacCer is the starting point in the biosynthesis of all gangliosides (membrane-bound glycosphingolipids) which play pivotal roles in the CNS including neuronal maturation and axonal and myelin formation (By similarity). Plays a role in the glycosylation of BMPR1A and regulation of its protein stability (By similarity). Essential for extraembryonic development during early embryogenesis (By similarity).
See full target information B4GALT5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 16:5 PubMed36611197

2023

A signature based on glycosyltransferase genes provides a promising tool for the prediction of prognosis and immunotherapy responsiveness in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xuyao Xu,Yue Wu,Genmei Jia,Qiaoying Zhu,Dake Li,Kaipeng Xie

Neuromolecular medicine 21:33-41 PubMed30607818

2019

Hsc70 Interacts with β4GalT5 to Regulate the Growth of Gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Guan Sun,Ying Cao,Xueliang Dai,Min Li,Jun Guo
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB155905

Anti-B4GALT5 antibody

primary-antibodies

b4galt5-antibody-ab155905

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