AB288418

Anti-B7-H6 antibody [17B1.3]

Recombinant
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Rabbit Recombinant Monoclonal B7-H6 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NCR3LG1.

View Alternative Names

B7H6, NCR3LG1, Natural cytotoxicity triggering receptor 3 ligand 1, B7 homolog 6, B7-H6

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-B7-H6 antibody [17B1.3] (AB288418)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-B7-H6 antibody [17B1.3] (AB288418)

Immunofluorescence staining of fixed K562 cells with ab288418 : Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix™ coverslips stained with ab288418 at 10 μg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 μg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom ab288418, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

17B1.3

Isotype

IgG

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NCR3LG1. The exact immunogen used to generate this antibody is proprietary information.

Q68D85

Specificity

mAb 17B1.3 specifically recognises B7-H6. 17B1.3 reacted with human tumour cell lines constitutively expressing B7-H6 such as HeLa cells. Fab 17B1.3 does not interfere with the binding of B7-H6 to NKp30, but nevertheless blocks NK cell activation.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Proclin 300 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

B7-H6 also called NCR3LG1 is a cell surface protein functioning as a ligand for the NKp30 receptor. The protein weighs approximately 55 kDa and is mainly expressed on tumor cells and certain activated immune cells. Scientists often use tools like B7-H6 flow assays to study its expression patterns in various cell types. In the immune landscape B7-H6 interacts with NKp30 to stimulate natural killer (NK) cell activation and mediate immune responses.

Biological function summary

B7-H6 acts as an important component in natural killer cell-mediated cytotoxicity. The protein is not part of a larger complex but operates independently to engage NK cell activity. It serves as a signal for NK cells to identify and destroy tumor cells. Additionally B7-H6 expression on tumor cells often correlates with immune cell recognition thereby playing an important role in tumor immunity.

Pathways

B7-H6 participates in the immune response pathways connected to natural killer cell activation. Specifically it interacts with proteins like NKp30 as part of the natural cytotoxicity triggering receptor signaling process. These interactions facilitate the destruction of target cells enhancing the body's innate immune response by activating downstream pathways vital to immune defense.

B7-H6 has associations with cancer and infectious diseases. In several cancer types including mesothelioma the protein's elevated expression can serve as an indicator of tumorigenesis. B7-H6 alongside its partner NKp30 potentially plays a role in immune escape mechanisms employed by tumors. Understanding this interaction offers insights into novel therapeutic strategies for tackling such conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Triggers NCR3-dependent natural killer cell activation.

See full target information NCR3LG1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-B7-H6 antibody [17B1.3]

Immunocytochemistry/ Immunofluorescence - Anti-B7-H6 antibody [17B1.3] (AB288418)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise