AB229999

Anti-B7-H6 antibody

Name: Anti-B7-H6 antibody (ab229999) | Abcam
Brand: Abcam Limited
SKU: AB229999

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(2 Publications)

Rabbit Polyclonal B7-H6 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NCR3LG1 aa 1-300.

View Alternative Names

B7H6, NCR3LG1, Natural cytotoxicity triggering receptor 3 ligand 1, B7 homolog 6, B7-H6

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7-H6 antibody (AB229999)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7-H6 antibody (AB229999)

Paraffin-embedded human melanoma tissue stained for B7-H6 using ab229999 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-B7-H6 antibody (AB229999)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-B7-H6 antibody (AB229999)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for B7-H6 (green) using ab229999 at 1/100 dilution in ICC/IF, followed by Alexa Fluor^® 488-conjugated Goat Anti-Rabbit IgG(H+L) as the secondary antibody.

Supplier Data

Western blot - Anti-B7-H6 antibody (AB229999)

All lanes:

Western blot - Anti-B7-H6 antibody (ab229999) at 1/2000 dilution

Lane 1:

Jurkat cell lysate

Lane 2:

PC-3 cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NCR3LG1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q68D85

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

B7-H6 also called NCR3LG1 is a cell surface protein functioning as a ligand for the NKp30 receptor. The protein weighs approximately 55 kDa and is mainly expressed on tumor cells and certain activated immune cells. Scientists often use tools like B7-H6 flow assays to study its expression patterns in various cell types. In the immune landscape B7-H6 interacts with NKp30 to stimulate natural killer (NK) cell activation and mediate immune responses.

Biological function summary

B7-H6 acts as an important component in natural killer cell-mediated cytotoxicity. The protein is not part of a larger complex but operates independently to engage NK cell activity. It serves as a signal for NK cells to identify and destroy tumor cells. Additionally B7-H6 expression on tumor cells often correlates with immune cell recognition thereby playing an important role in tumor immunity.

Pathways

B7-H6 participates in the immune response pathways connected to natural killer cell activation. Specifically it interacts with proteins like NKp30 as part of the natural cytotoxicity triggering receptor signaling process. These interactions facilitate the destruction of target cells enhancing the body's innate immune response by activating downstream pathways vital to immune defense.

B7-H6 has associations with cancer and infectious diseases. In several cancer types including mesothelioma the protein's elevated expression can serve as an indicator of tumorigenesis. B7-H6 alongside its partner NKp30 potentially plays a role in immune escape mechanisms employed by tumors. Understanding this interaction offers insights into novel therapeutic strategies for tackling such conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Triggers NCR3-dependent natural killer cell activation.

See full target information NCR3LG1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular endocrinology 584:112160 PubMed38266771

2024

PMM2 controls ERα levels and cell proliferation in ESR1 Y537S variant expressing breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Manuela Cipolletti,Filippo Acconcia

Biomarkers : biochemical indicators of exposure, r :1-20 PubMed33523715

2021

NCR3LG1(B7-H6) is a potential prognostic factor for bladder cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Zhao,Tong Lian,Jianmin Li,Shiyao Wei,Hongjie Li,Changying Li,Haitao Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-B7-H6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7-H6 antibody (AB229999)

Immunocytochemistry/ Immunofluorescence - Anti-B7-H6 antibody (AB229999)

Western blot - Anti-B7-H6 antibody (AB229999)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise