Anti-B7-H6 antibody [EPR21841]

• WB

Supplier Data

Western blot - Anti-B7-H6 antibody [EPR21841] (AB219205)

Blocking/Dilution buffer : 5% NFDM/TBST.

MCF7 cells have a low level of B7-H6 expression. The molecular mass observed is consistent with the literature (PMID 24780758).

All lanes:

Western blot - Anti-B7-H6 antibody [EPR21841] (ab219205) at 1/1000 dilution

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

Lane 2:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 51 kDa

Observed band size: 80 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-B7-H6 antibody [EPR21841] (AB219205)

Exposure time : Lanes 1 and 2 : 92 seconds; Lanes 3 and 4 : 3 minutes.

Blocking/Dilution buffer : 5% NFDM/TBST.

B7-H6 is a glycoprotein and can be de-glycosylated by PNGase F. The molecular mass observed is consistent with the literature (PMID 24780758).

All lanes:

Western blot - Anti-B7-H6 antibody [EPR21841] (ab219205) at 1/1000 dilution

Lane 1:

Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

HepG2 whole cell lysate treated with PNGase F at 20 µg

Lane 3:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 51 kDa

Observed band size: 50-80 kDa

false