Skip to main content

Rabbit Recombinant Monoclonal B7-H6 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-B7-H6 antibody [EPR28658-68] - BSA and Azide free (AB316976), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7-H6 antibody [EPR28658-68] - BSA and Azide free (AB316976), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7-H6 antibody [EPR28658-68] - BSA and Azide free (AB316976), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7-H6 antibody [EPR28658-68] - BSA and Azide free (AB316976), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7-H6 antibody [EPR28658-68] - BSA and Azide free (AB316976), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt
Human
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Triggers NCR3-dependent natural killer cell activation.

Alternative names

B7H6, NCR3LG1, Natural cytotoxicity triggering receptor 3 ligand 1, B7 homolog 6, B7-H6

Recommended products

Rabbit Recombinant Monoclonal B7-H6 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28658-68
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab316976 is the carrier-free version of Anti-B7-H6 antibody [EPR28658-68] ab316975.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

B7-H6 also called NCR3LG1 is a cell surface protein functioning as a ligand for the NKp30 receptor. The protein weighs approximately 55 kDa and is mainly expressed on tumor cells and certain activated immune cells. Scientists often use tools like B7-H6 flow assays to study its expression patterns in various cell types. In the immune landscape B7-H6 interacts with NKp30 to stimulate natural killer (NK) cell activation and mediate immune responses.

Biological function summary

B7-H6 acts as an important component in natural killer cell-mediated cytotoxicity. The protein is not part of a larger complex but operates independently to engage NK cell activity. It serves as a signal for NK cells to identify and destroy tumor cells. Additionally B7-H6 expression on tumor cells often correlates with immune cell recognition thereby playing an important role in tumor immunity.

Pathways

B7-H6 participates in the immune response pathways connected to natural killer cell activation. Specifically it interacts with proteins like NKp30 as part of the natural cytotoxicity triggering receptor signaling process. These interactions facilitate the destruction of target cells enhancing the body's innate immune response by activating downstream pathways vital to immune defense.

Associated diseases and disorders

B7-H6 has associations with cancer and infectious diseases. In several cancer types including mesothelioma the protein's elevated expression can serve as an indicator of tumorigenesis. B7-H6 alongside its partner NKp30 potentially plays a role in immune escape mechanisms employed by tumors. Understanding this interaction offers insights into novel therapeutic strategies for tackling such conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-B7-H6 antibody [EPR28658-68] - BSA and Azide free (ab316976), expandable thumbnail

    Western blot - Anti-B7-H6 antibody [EPR28658-68] - BSA and Azide free (ab316976)

    This data was developed using Anti-B7-H6 antibody [EPR28658-68] ab316975, the same antibody clone in a different buffer formulation.

    Negative control: MCF7 (PMID: 22851709, PMID: 24780758), Daudi.

    Low expression: A549, HCT 116 (PMID: 24780758).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-B7-H6 antibody [EPR28658-68] (Anti-B7-H6 antibody [EPR28658-68] ab316975) at 1/1000 dilution

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 3: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 4: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 5: Daudi (human burkitts lymphoma lymphoblast) whole cell lysate at 20 µg with NFDM/TBST

    Lane 6: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 7: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 80 kDa, 36 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7-H6 antibody [EPR28658-68] - BSA and Azide free (ab316976), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7-H6 antibody [EPR28658-68] - BSA and Azide free (ab316976)

    This data was developed using Anti-B7-H6 antibody [EPR28658-68] ab316975, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling B7-H6 with Anti-B7-H6 antibody [EPR28658-68] ab316975 at 1/100 (5.08 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human breast cancer.

    The section was incubated with Anti-B7-H6 antibody [EPR28658-68] ab316975 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7-H6 antibody [EPR28658-68] - BSA and Azide free (ab316976), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7-H6 antibody [EPR28658-68] - BSA and Azide free (ab316976)

    This data was developed using Anti-B7-H6 antibody [EPR28658-68] ab316975, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling B7-H6 with Anti-B7-H6 antibody [EPR28658-68] ab316975 at 1/100 (5.08 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in human lung (PMID: 25400778).

    The section was incubated with Anti-B7-H6 antibody [EPR28658-68] ab316975 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7-H6 antibody [EPR28658-68] - BSA and Azide free (ab316976), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7-H6 antibody [EPR28658-68] - BSA and Azide free (ab316976)

    This data was developed using Anti-B7-H6 antibody [EPR28658-68] ab316975, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded (A) HepG2 (human hepatocellular carcinoma epithelial cell) cell pellet. (B) K562 (Human chronic myelogenous leukemia lymphoblast) cell pellets; (C) MCF-7 (human breast adenocarcinoma epithelial cell) cell pellet; (D) Daudi (human Burkitt's lymphoma lymphoblast) cell pellets; tissue labeling B7-H6 with Anti-B7-H6 antibody [EPR28658-68] ab316975 at 1/500 (1.016 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in (A) HepG2 and (B) K562, no staining in (C) MCF-7 and (D) Daudi.

    The section was incubated with Anti-B7-H6 antibody [EPR28658-68] ab316975 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7-H6 antibody [EPR28658-68] - BSA and Azide free (ab316976), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7-H6 antibody [EPR28658-68] - BSA and Azide free (ab316976)

    This data was developed using Anti-B7-H6 antibody [EPR28658-68] ab316975, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human B cell lymphoma tissue labeling B7-H6 with Anti-B7-H6 antibody [EPR28658-68] ab316975 at 1/100 (5.08 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human B cell lymphoma (PMID: 19528259).

    The section was incubated with Anti-B7-H6 antibody [EPR28658-68] ab316975 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7-H6 antibody [EPR28658-68] - BSA and Azide free (ab316976), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7-H6 antibody [EPR28658-68] - BSA and Azide free (ab316976)

    This data was developed using Anti-B7-H6 antibody [EPR28658-68] ab316975, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling B7-H6 with Anti-B7-H6 antibody [EPR28658-68] ab316975 at 1/100 (5.08 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human astrocytoma (PMID: 27330308).

    The section was incubated with Anti-B7-H6 antibody [EPR28658-68] ab316975 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com