Rabbit Recombinant Monoclonal B7H4 antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Human samples.
Images
![Sandwich ELISA - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (AB244746), expandable thumbnail](https://content.abcam.com/products/images/b7h4-antibody-epr20236-9-bsa-and-azide-free-ab244746--sandwich-elisa-img52959.png/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (AB244746)")
![Sandwich ELISA - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (AB244746), expandable thumbnail](https://content.abcam.com/products/images/b7h4-antibody-epr20236-9-bsa-and-azide-free-ab244746--sandwich-elisa-img51606.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (AB244746)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
| Mouse | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.
Alternative names
B7H4, UNQ659/PRO1291, VTCN1, V-set domain-containing T-cell activation inhibitor 1, B7 homolog 4, B7h.5, Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7-H4
Recommended products
Rabbit Recombinant Monoclonal B7H4 antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20236-9
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab244746 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human B7-H4. The recommended pair for sandwich ELISA is:
Capture: ab244746, Human B7-H4 Capture Antibody (unconjugated)
Detector: Anti-B7H4 antibody [EPR20236-301] - BSA and Azide free (Detector) ab245016, Human B7-H4 Detector Antibody (unconjugated)
The reference range value is 23 - 1500 pg/ml.
Or alternatively, for Mouse/Rat, the recommended pair for sandwich ELISA is:
Capture: Anti-B7H4 antibody [EPR21019-229] - BSA and Azide free (Capture) ab242918, Mouse/Rat B7-H4 Capture Antibody (unconjugated)
Detector: ab244746, Mouse/Rat B7-H4 Detector Antibody (unconjugated)
The reference range value is 46.875 - 3000 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
B7-H4 also known as VTCN1 or B7x is a member of the B7 family of immunoregulatory proteins. It has an approximate molecular mass of 50 kDa. B7-H4 expression is detected on the surface of various cells including B cells T cells monocytes and some cancer cells. Its expression is often inducible; immune cells can express B7-H4 in response to specific conditions or signals in the environment.
- Biological function summary
B7-H4 acts as an immune checkpoint molecule that negatively regulates T cell immunity. It does not form a known complex with other proteins but instead interacts directly with T cells. By inhibiting T cell activation and proliferation B7-H4 contributes to the regulation of immune responses helping to maintain self-tolerance and prevent autoimmunity.
- Pathways
B7-H4 is involved in the regulation of immune checkpoints prominently in the pathways related to immune tolerance and suppression. It plays a critical role in the pathways that modulate tumor evasion of the immune system. B7-H4 is related to other immune checkpoint proteins such as PD-L1 which are also pivotal in immune regulation and therapy targets.
- Associated diseases and disorders
B7-H4 is connected to cancer progression and autoimmune conditions. Overexpression of B7-H4 in certain cancers such as ovarian and breast cancer associates with tumor immune evasion allowing tumors to grow unchecked by the immune system. Its expression links with proteins like CTLA-4 in maintaining immune suppression. B7-H4 modifications could serve as a potential target in cancer immunotherapy aiming to enhance the immune system's ability to combat malignancies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Sandwich ELISA - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (ab244746), expandable thumbnail](https://content.abcam.com/products/images/b7h4-antibody-epr20236-9-bsa-and-azide-free-ab244746--sandwich-elisa-img52959.png "Sandwich ELISA - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (ab244746)")
Sandwich ELISA - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (ab244746)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Mouse/Rat B7-H4 ELISA Kit ab263879).
![Sandwich ELISA - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (ab244746), expandable thumbnail](https://content.abcam.com/products/images/b7h4-antibody-epr20236-9-bsa-and-azide-free-ab244746--sandwich-elisa-img51606.jpg "Sandwich ELISA - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (ab244746)")
Sandwich ELISA - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (ab244746)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Human B7-H4 ELISA Kit ab233633).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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