Rabbit Recombinant Monoclonal B7H4 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human, Mouse samples.
Images
![Flow Cytometry - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (AB269460), expandable thumbnail](https://content.abcam.com/products/images/b7h4-antibody-epr20236-9-bsa-and-azide-free-ab269460--flow-cytometry-img71602.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (AB269460)")
![Immunoprecipitation - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (AB269460), expandable thumbnail](https://content.abcam.com/products/images/b7h4-antibody-epr20236-9-bsa-and-azide-free-ab269460--immunoprecipitation-img3200.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (AB269460)")
![Immunocytochemistry/ Immunofluorescence - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (AB269460), expandable thumbnail](https://content.abcam.com/products/images/b7h4-antibody-epr20236-9-bsa-and-azide-free-ab269460--immunocytochemistry-immunofluorescence-img18871.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (AB269460)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Not recommended | Tested |
| Mouse | Predicted | Predicted | Expected | Not recommended | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.
Alternative names
B7H4, UNQ659/PRO1291, VTCN1, V-set domain-containing T-cell activation inhibitor 1, B7 homolog 4, B7h.5, Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7-H4
Recommended products
Rabbit Recombinant Monoclonal B7H4 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20236-9
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab269460 is the carrier-free version of Anti-B7H4 antibody [EPR20236-9] ab269455.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
B7-H4 also known as VTCN1 or B7x is a member of the B7 family of immunoregulatory proteins. It has an approximate molecular mass of 50 kDa. B7-H4 expression is detected on the surface of various cells including B cells T cells monocytes and some cancer cells. Its expression is often inducible; immune cells can express B7-H4 in response to specific conditions or signals in the environment.
- Biological function summary
B7-H4 acts as an immune checkpoint molecule that negatively regulates T cell immunity. It does not form a known complex with other proteins but instead interacts directly with T cells. By inhibiting T cell activation and proliferation B7-H4 contributes to the regulation of immune responses helping to maintain self-tolerance and prevent autoimmunity.
- Pathways
B7-H4 is involved in the regulation of immune checkpoints prominently in the pathways related to immune tolerance and suppression. It plays a critical role in the pathways that modulate tumor evasion of the immune system. B7-H4 is related to other immune checkpoint proteins such as PD-L1 which are also pivotal in immune regulation and therapy targets.
- Associated diseases and disorders
B7-H4 is connected to cancer progression and autoimmune conditions. Overexpression of B7-H4 in certain cancers such as ovarian and breast cancer associates with tumor immune evasion allowing tumors to grow unchecked by the immune system. Its expression links with proteins like CTLA-4 in maintaining immune suppression. B7-H4 modifications could serve as a potential target in cancer immunotherapy aiming to enhance the immune system's ability to combat malignancies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (ab269460), expandable thumbnail](https://content.abcam.com/products/images/b7h4-antibody-epr20236-9-bsa-and-azide-free-ab269460--flow-cytometry-img71602.jpg "Flow Cytometry - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (ab269460)")
Flow Cytometry - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (ab269460)
Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized HEK-293T (human embryonic kidney epithelial cell) transfected with myc-tagged B7-H4 expression vector cells labelling B7H4 with Anti-B7H4 antibody [EPR20236-9] ab269455 at 1/60 dilution (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left). PMID: 23722540 and PMID: 27632942 showed that cell surface expression of B7-H4 hard to detected in vitro cultured cell lines, and all the commercial B7H4 antibodies that recommend FC as an application use the overexpression system. Our RabMab also could recognise overexpressed B7H4.The overexpression plasmid contains the full length aa1-282 of human B7H4(Q7Z7D3) with HA tag in pcDNA3.1(+) vector.
Cells were surface stained with rabbit IgG (Left) or Anti-B7H4 antibody [EPR20236-9] ab269455 (Right). Then fixed with 2% PFA followed by intracellularly stained with anti-HA tag conjugated to Alexa Fluor® 647.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-B7H4 antibody [EPR20236-9] ab269455).
![Immunoprecipitation - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (ab269460), expandable thumbnail](https://content.abcam.com/products/images/b7h4-antibody-epr20236-9-bsa-and-azide-free-ab269460--immunoprecipitation-img3200.jpg "Immunoprecipitation - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (ab269460)")
Immunoprecipitation - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (ab269460)
B7H4 was immunoprecipitated from 0.35 mg SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate with Anti-B7H4 antibody [EPR20236-9] ab269455 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-B7H4 antibody [EPR20236-9] ab269455 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: SK-BR-3 whole cell lysate 10ug.
Lane 2: Anti-B7H4 antibody [EPR20236-9] ab269455 IP in SK-BR-3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-B7H4 antibody [EPR20236-9] ab269455 in SK-BR-3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
B7H4 is N-glycosylated protein. The level of B7H4 glycosylation varies between different cells and tissues. The band at 65kDa represents glycosylated B7H4. The band at 28kDa represents non-glycosylated B7H4 (PMID: 15878339).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-B7H4 antibody [EPR20236-9] ab269455).
All lanes: Immunoprecipitation - Anti-B7H4 antibody [EPR20236-9] (Anti-B7H4 antibody [EPR20236-9] ab269455)
Predicted band size: 31 kDa
Observed band size: 28 kDa, 65 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (ab269460), expandable thumbnail](https://content.abcam.com/products/images/b7h4-antibody-epr20236-9-bsa-and-azide-free-ab269460--immunocytochemistry-immunofluorescence-img18871.jpg "Immunocytochemistry/ Immunofluorescence - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (ab269460)")
Immunocytochemistry/ Immunofluorescence - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (ab269460)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling B7H4 with Anti-B7H4 antibody [EPR20236-9] ab269455 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in HEK-293T cell line transfected with a HA-tagged B7-H4 expression vector. Alexa Fluor® 647 anti-HA.11 Epitope Tag Antibody was used at 200 dilution to detecte the overexpressed protein (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluo®488 Goat anti-Rabbit secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-B7H4 antibody [EPR20236-9] ab269455).
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