AB171280

Anti-B7H4 antibody [H74] - Low endotoxin, Azide free

Name: Anti-B7H4 antibody [H74] - Low endotoxin, Azide free (ab171280) | Abcam
Brand: Abcam Limited
SKU: AB171280
Price: 460 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal B7H4 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Full Length Protein corresponding to Human VTCN1.

View Alternative Names

B7H4, UNQ659/PRO1291, VTCN1, V-set domain-containing T-cell activation inhibitor 1, B7 homolog 4, B7h.5, Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7-H4

1 Images

Flow Cytometry - Anti-B7H4 antibody [H74] - Low endotoxin, Azide free (AB171280)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-B7H4 antibody [H74] - Low endotoxin, Azide free (AB171280)

Flow cytometric analysis of non-transfected (left) and Human B7H4 transfected (right) 293T cells with 0.125 μg of Mouse IgG1 K Isotype Control (open histogram) or 0.125 μg of ab171280 (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE. Total cells were used for analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

H74

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Full Length Protein corresponding to Human VTCN1. The exact immunogen used to generate this antibody is proprietary information.

Q7Z7D3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.125-0.25 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>(in a final volume of 100 μl).</p><p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by the LAL assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

B7-H4 also known as VTCN1 or B7x is a member of the B7 family of immunoregulatory proteins. It has an approximate molecular mass of 50 kDa. B7-H4 expression is detected on the surface of various cells including B cells T cells monocytes and some cancer cells. Its expression is often inducible; immune cells can express B7-H4 in response to specific conditions or signals in the environment.

Biological function summary

B7-H4 acts as an immune checkpoint molecule that negatively regulates T cell immunity. It does not form a known complex with other proteins but instead interacts directly with T cells. By inhibiting T cell activation and proliferation B7-H4 contributes to the regulation of immune responses helping to maintain self-tolerance and prevent autoimmunity.

Pathways

B7-H4 is involved in the regulation of immune checkpoints prominently in the pathways related to immune tolerance and suppression. It plays a critical role in the pathways that modulate tumor evasion of the immune system. B7-H4 is related to other immune checkpoint proteins such as PD-L1 which are also pivotal in immune regulation and therapy targets.

B7-H4 is connected to cancer progression and autoimmune conditions. Overexpression of B7-H4 in certain cancers such as ovarian and breast cancer associates with tumor immune evasion allowing tumors to grow unchecked by the immune system. Its expression links with proteins like CTLA-4 in maintaining immune suppression. B7-H4 modifications could serve as a potential target in cancer immunotherapy aiming to enhance the immune system's ability to combat malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.

See full target information VTCN1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Particle and fibre toxicology 13:16 PubMed27026438

2016

Multi-walled carbon nanotube induces nitrative DNA damage in human lung epithelial cells via HMGB1-RAGE interaction and Toll-like receptor 9 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Hiraku,Feiye Guo,Ning Ma,Tatsuhiko Yamada,Shumin Wang,Shosuke Kawanishi,Mariko Murata

View all publications

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