JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB154918

Anti-BAAT antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal BAAT antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Bile acid-CoA:amino acid N-acyltransferase.

View Alternative Names

Bile acid-CoA:amino acid N-acyltransferase, BACAT, BAT, Bile acid-CoA thioesterase, Choloyl-CoA hydrolase, Glycine N-choloyltransferase, Long-chain fatty-acyl-CoA hydrolase, BAAT

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAAT antibody (AB154918)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAAT antibody (AB154918)

Paraffin-embedded human hepatoma tissue stained for BAAT using ab154918 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval step : EDTA based pH 8.0 buffer, 15 mins.

Western blot - Anti-BAAT antibody (AB154918)
  • WB

Unknown

Western blot - Anti-BAAT antibody (AB154918)

10% SDS PAGE

All lanes:

Western blot - Anti-BAAT antibody (ab154918) at 1/1000 dilution

Lane 1:

A431 (Human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human Bile acid-CoA:amino acid N-acyltransferase. The exact immunogen used to generate this antibody is proprietary information.

Q14032

style
left-column

Complementary Products

Primary Antibodies

AB156879

Anti-Spermine synthase antibody [EPR9252(B)]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Spermine synthase antibody [EPR9252(B)] (AB156879)

  • 0
  • 0
Primary Antibodies

AB176562

Anti-Glutamine Synthetase antibody [EPR13022(B)]

BOND RX™ Validated|20ul selling size|Recombinant|KO Validated|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [EPR13022(B)] (AB176562)

  • 5
  • 4
Primary Antibodies

AB228978

Anti-SLAM / CD150 antibody [EPR22904-135]

BOND RX™ Validated|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAM / CD150 antibody [EPR22904-135] (AB228978)

  • 0
  • 0
Primary Antibodies

AB246917

Anti-LIAS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIAS antibody (AB246917)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BAAT also known as Bile Acid-CoA: Amino Acid N-Acyltransferase is an enzyme that catalyzes the transfer of amino acids to bile acids forming conjugated bile acids. This process increases bile acid solubility and facilitates its function. BAAT has a molecular mass of approximately 47 kDa and is highly expressed in the liver. This expression is central for the enzyme's role in facilitating bile acid processing which is critical in fat digestion and absorption.
Biological function summary

BAAT plays an important role in conjugating bile acids with amino acids such as glycine and taurine. This conjugation modifies the hydrophobicity and toxicity of bile acids making them more water-soluble. BAAT operates within the mitochondria and functions as an independent enzyme rather than being part of a larger enzymatic complex. Its essential role lies in maintaining the homeostasis of bile acids which is important for lipid metabolism.

Pathways

BAAT is a part of the bile acid biosynthesis pathway. It assists in the conversion process that ensures the enterohepatic circulation of bile acids. Within this pathway BAAT works closely with enzymes like the cytochrome P450 family especially CYP7A1 which initiate bile acid synthesis. This partnership maintains the cycle of conversion and reabsorption of bile acids ensuring their availability for dietary fat processing.

BAAT relates significantly to disorders affecting bile acid metabolism such as cholestasis and gallstones. Abnormalities or deficiencies in BAAT activity can disrupt bile acid homeostasis contributing to these conditions. Moreover BAAT dysfunction is closely associated with defects in CYP7A1 as both enzymes play interconnected roles in bile acid synthesis and regulation. Understanding these interactions can provide insights into therapeutic approaches for bile-related disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the amidation of bile acids (BAs) with the amino acids taurine and glycine (PubMed : 12239217, PubMed : 12810727, PubMed : 2037576, PubMed : 8034703). More than 95% of the BAs are N-acyl amidates with glycine and taurine (PubMed : 8034703). Amidation of BAs in the liver with glycine or taurine prior to their excretion into bile is an important biochemical event in bile acid metabolism (PubMed : 12810727). This conjugation (or amidation) plays several important biological roles in that it promotes the secretion of BAs and cholesterol into bile and increases the detergent properties of BAs in the intestine, which facilitates lipid and vitamin absorption (PubMed : 12810727). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids (PubMed : 12239217, PubMed : 12810727, PubMed : 8034703). In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs (PubMed : 12810727).
See full target information Bile acid-CoA:amino acid N-acyltransferase
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational pediatrics 10:2083-2094 PubMed34584879

2021

Identification and evaluation of fructose-bisphosphate aldolase B as a potential diagnostic biomarker in choledochal cysts patients: a quantitative proteomic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Gedong Ming,Wanliang Guo,Yuan Cheng,Jian Wang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com