Anti-BACE1 antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (AB10716)

Immunocytochemistry/Immunofluorescent analysis of BACE1 (green) showing staining in the Golgi apparatus of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab10716 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (AB10716)

Immunocytochemistry/Immunofluorescent analysis of BACE1 (green) showing staining in the Golgi apparatus of SH-SY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a10716 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (AB10716)

Immunocytochemistry/Immunofluorescent analysis of BACE1 (green) showing staining in the Golgi apparatus of Neuro-2a cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab10716 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

• WB

Supplier Data

Western blot - Anti-BACE1 antibody (AB10716)

Western blot analysis of BACE1 was performed by loading Samples onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with primary antibody overnight at 4°C, washed in TBST, and probed with secondary antibody for 1 hour at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate.

All lanes:

Western blot - Anti-BACE1 antibody (ab10716) at 1/1000 dilution

Lane 1:

U87-MG cell lysate at 25 µg

Lane 2:

HeLa cell lysate at 25 µg

Lane 3:

Mouse brain cell lysate at 25 µg

Secondary

All lanes:

HRP-conjugated secondary antibody

Predicted band size: 56 kDa

Observed band size: 42 kDa,56 kDa,70 kDa

false