Rabbit Polyclonal BACE1 antibody. Suitable for ICC, WB, IHC-P and reacts with Mouse, Human samples. Cited in 91 publications. Immunogen corresponding to Synthetic Peptide within Human BACE1.
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- Brain sciences 13:2023DEAD-Box Helicase 17 Promotes Amyloidogenesis by Regulating BACE1 Translation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue Liu,Guifeng Zhou,Li Song,Qixin Wen,Shiqi Xie,Long Chen,Lu Wang,Xiaoyong Xie,Xue Chen,Yalan Pu,Guojun ChenPubMed 37239217
- International journal of molecular sciences 24:2023Oleracone F Alleviates Cognitive Impairment and Neuropathology in APPswe/PSEN1dE9 Mice by Reducing the Expression of Vascular Cell Adhesion Molecule and Leukocyte Adhesion to Brain Vascular Endothelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoung-Sun Kwon,Jin-Sung Ko,Se-Young Oh,Young Taek Han,Sangmee Ahn JoPubMed 36768379
- Scientific reports 12:220282022The interrelationships between neuronal viability, synaptic integrity, microglial responses, and amyloid-beta formation in an in vitro neurotrauma model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLan-Wan Wang,Hung-Jung Lin,Chien-Ming Chao,Mao-Tsun Lin,Lin-Yu Wang,Lan-Hsiang Chein,Ching-Ping Chang,Chung-Ching ChioPubMed 36539544
- Traffic (Copenhagen, Denmark) 24:20-332022AP2S1 regulates APP degradation through late endosome-lysosome fusion in cells and APP/PS1 mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi-Xin Wen,Biao Luo,Xiao-Yong Xie,Gui-Feng Zhou,Jian Chen,Li Song,Yue Liu,Shi-Qi Xie,Long Chen,Kun-Yi Li,Xiao-Jiao Xiang,Guo-Jun ChenPubMed 36412210
- EMBO molecular medicine 15:e167892022Whole-brain microscopy reveals distinct temporal and spatial efficacy of anti-Aβ therapies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Kirschenbaum,Ehsan Dadgar-Kiani,Francesca Catto,Fabian F Voigt,Chiara Trevisan,Oliver Bichsel,Hamid Shirani,K Peter R Nilsson,Karl J Frontzek,Paolo Paganetti,Fritjof Helmchen,Jin Hyung Lee,Adriano AguzziPubMed 36382364
- Frontiers in nutrition 9:8925582022Inhibitory Effects of Macelignan on Tau Phosphorylation and Aβ Aggregation in the Cell Model of Alzheimer's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Gu,Nan Cai,Meiting Li,Decheng Bi,Lijun Yao,Weishan Fang,Yan Wu,Zhangli Hu,Qiong Liu,Zhijian Lin,Jun Lu,Xu XuPubMed 35662922
- Aging cell 21:e135932022Reducing Nav1.6 expression attenuates the pathogenesis of Alzheimer's disease by suppressing BACE1 transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDe-Juan Yuan,Guang Yang,Wei Wu,Qi-Fa Li,De-En Xu,Michael Ntim,Chun-Yan Jiang,Ji-Chuan Liu,Yue Zhang,Ying-Zi Wang,Dan-Dan Zhu,Supratik Kundu,Ai-Ping Li,Zhi-Cheng Xiao,Quan-Hong Ma,Shao LiPubMed 35353937
- Redox biology 51:1022802022Theranostic F-SLOH mitigates Alzheimer's disease pathology involving TFEB and ameliorates cognitive functions in Alzheimer's disease models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshok Iyaswamy,Xueli Wang,Senthilkumar Krishnamoorthi,Venkatapathy Kaliamoorthy,Sravan G Sreenivasmurthy,Siva Sundara Kumar Durairajan,Ju-Xian Song,Benjamin Chun-Kit Tong,Zhou Zhu,Cheng-Fu Su,Jia Liu,King-Ho Cheung,Jia-Hong Lu,Jie-Qiong Tan,Hung Wing Li,Man Shing Wong,Min LiPubMed 35286997
- Cell death discovery 7:3122021Cell death inhibitors protect against brain damage caused by cardiac ischemia/reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuchan Liao,Nattayaporn Apaijai,Ying Luo,Jun Wu,Titikorn Chunchai,Kodchanan Singhanat,Busarin Arunsak,Juthipong Benjanuwattra,Nipon Chattipakorn,Siriporn C ChattipakornPubMed 34689160
- Neuroscience bulletin 38:290-3022021Polysaccharide Krestin Prevents Alzheimer's Disease-type Pathology and Cognitive Deficits by Enhancing Monocyte Amyloid-β Processing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSi-Han Chen,Chen-Yang He,Ying-Ying Shen,Gui-Hua Zeng,Ding-Yuan Tian,Yuan Cheng,Man-Yu Xu,Dong-Yu Fan,Cheng-Rong Tan,An-Yu Shi,Xian-Le Bu,Yan-Jiang WangPubMed 34611829
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human BACE1. The exact immunogen used to generate this antibody is proprietary information. Database link P56817
Consider this alternative
Reactivity data
Select an application| ICC | WB | IHC-P | |
|---|---|---|---|
| Human | Expected | Tested | Expected |
| Mouse | Tested | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes We recommend overnight incubation at 4C and using 5% skim milk to block. |
Species Human | Dilution info 1 µg/mL | Notes We recommend overnight incubation at 4C and using 5% skim milk to block. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2.5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
2 products for Alternative Version
Target data
Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase (PubMed:10656250, PubMed:10677483, PubMed:20354142). Cleaves CHL1 (By similarity).
Alternative names
BACE, KIAA1149, BACE1, Beta-secretase 1, Aspartyl protease 2, Beta-site amyloid precursor protein cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2, ASP2, Asp 2, Beta-site APP cleaving enzyme 1
Recommended products
Rabbit Polyclonal BACE1 antibody. Suitable for ICC, WB, IHC-P and reacts with Mouse, Human samples. Cited in 91 publications. Immunogen corresponding to Synthetic Peptide within Human BACE1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human BACE1. The exact immunogen used to generate this antibody is proprietary information. Database link P56817
- Purification technique
- Affinity purification
- Concentration
- Purification notes
BACE Antibody is Ion exchange chromatography purified.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
BACE1 also known as beta-site APP cleaving enzyme 1 or beta-secretase plays an important role in the cleavage of amyloid precursor protein (APP). This cleavage results in the production of amyloid-beta peptides which are associated with Alzheimer's disease. BACE1 is a membrane-bound aspartic protease and has a molecular weight of approximately 50100 Da. The enzyme expresses mostly in the brain's neurons and some secretory tissues.
- Biological function summary
BACE1 initiates the amyloidogenic pathway of APP processing which involves amyloid-beta generation. BACE1 doesn't function alone but acts as part of a complex that aids in protein substrate recognition and processing. Its activity contributes to physiological processes like myelination and axonal guidance indicating its importance beyond amyloid production.
- Pathways
BACE1 is integral to the amyloidogenic pathway which is important in Alzheimer's disease development. It interacts with proteins such as presenilin 1 a part of the gamma-secretase complex that further processes the amyloid-beta precursor. Furthermore BACE1 links to synaptic functions and neural signaling pathways highlighting its multifaceted roles.
- Associated diseases and disorders
BACE1 holds significance in Alzheimer's disease due to its role in amyloid-beta peptide production. This connection has led to the development of BACE1 inhibitors as potential therapeutic agents. Additionally BACE1’s involvement in other neural functions ties it to cognitive impairments. It also relates to APP through its function in Alzheimer's suggesting targeted strategies for treatment could involve modulating BACE1 activity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Image courtesy of an anonymous customer review.Immunocytochemistry - Anti-BACE1 antibody (ab2077)
Immunocytochemical analysis of D54MG (human glioblastoma cell line) cells labeling BACE1 with ab2077.Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab2077 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI.
Immunocytochemistry - Anti-BACE1 antibody (ab2077)
Immunocytochemical analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling BACE1 with ab2077 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Image showing both membrane and cytosol staining on NIH/3T3 cells.
Immunocytochemistry - Anti-BACE1 antibody (ab2077)
Immunocytochemical analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling BACE1 with ab2077 at 10 μg/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4oC. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western blot - Anti-BACE1 antibody (ab2077)
10% gel.
Running conditions: 130v for 2 hours.
Transfer conditions: wet, 250mA, 2 hrs (Nitrocellulose membrane).
Blocking condition: 5% non-fat dry milk in TBS, 4C, overnight.
Primary antibody incubation: Room temperature for 1 hour.
Secondary antibody incubation: Room temperature for 1 hour.
Washing conditions: 15 mL TSBT, 3 x 10 minutes.
Exposure: ECL solution
All lanes: Western blot - Anti-BACE1 antibody (ab2077) at 1 µg/mL
Lane 1: A431 (Human epidermoid carcinoma cell line) whole cell lysate at 15 µg
Lane 2: A549 (Human lung carcinoma cell line) whole cell lysate at 15 µg
Lane 3: Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate at 15 µg
Lane 4: Daudi (Human Burkitt's lymphoma cell line) whole cell lysate at 15 µg
Lane 5: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 6: K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 15 µg
Lane 7: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg
Lane 8: SK-N-SH (Human neuroblastoma cell line) whole cell lysate at 15 µg
Lane 9: THP-1 (Human monocytic leukemia cell line) whole cell lysate at 15 µg
SecondaryAll lanes: Rabbit IgG antibody (HRP) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 56 kDa
Observed band size: 65 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody (ab2077)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using ab2077 at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western blot - Anti-BACE1 antibody (ab2077)
Incubate the antibody for 1 hour at room temperature in 5% NFDM/TBST.
All lanes: Western blot - Anti-BACE1 antibody (ab2077) at 1 µg/mL
Lane 1: Human brain tissue lysate at 15 µg with absence of blocking peptide
Lane 2: Human brain tissue lysate at 15 µg with BACE1 peptide (ab7883)
Lane 3: Mouse 3T3/NIH cell lysate at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 56 kDa
Observed band size: 70 kDa
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