Anti-BACE1 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody (AB2077)

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-BACE antibody (ab2077) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as secondary. Counter stained with Hematoxylin.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (AB2077)

Immunocytochemical analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling BACE1 with ab2077 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Image showing both membrane and cytosol staining on NIH/3T3 cells.

• ICC

Supplier Data

Immunocytochemistry - Anti-BACE1 antibody (AB2077)

Immunocytochemical analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling BACE1 with ab2077 at 10 μg/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4^oC. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody (AB2077)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using ab2077 at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• ICC/IF

AbReview25871****

Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (AB2077)

Immunocytochemical analysis of D54MG (human glioblastoma cell line) cells labeling BACE1 with ab2077.Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab2077 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI.

Image courtesy of an anonymous Abreview.

• WB

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Western blot - Anti-BACE1 antibody (AB2077)

All lanes:

Western blot - Anti-BACE1 antibody (ab2077) at 1 µg/mL

Lane 1:

A431 cell lysates at 10 µg

Lane 2:

A549 cell lysates at 10 µg

Lane 3:

CaCo-2 cell lysates at 10 µg

Lane 4:

Daudi cell lysates at 10 µg

Lane 5:

HeLa cell lysates at 10 µg

Lane 6:

K562 cell lysates at 10 µg

Lane 7:

Jurkat cell lysates at 10 µg

Lane 8:

SK-N-SH cell lysates at 10 µg

Lane 9:

THP-1 cell lysates at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 55 kDa

Observed band size: 65 kDa

false

• WB

Supplier Data

Western blot - Anti-BACE1 antibody (AB2077)

All lanes:

Western blot - Anti-BACE1 antibody (ab2077) at 2 µg/mL

Lane 1:

Mouse bladder tissue lysates at 15 µg

Lane 2:

Mouse lung tissue lysates at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 55 kDa

Observed band size: 65 kDa

false