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Rabbit Recombinant Monoclonal BACE1 antibody. Carrier free. Suitable for mIHC, WB, IHC-P and reacts with Mouse, Rat, Human, Recombinant full length protein - Human samples.

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Images

Western blot - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (AB267796), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (AB267796), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (AB267796), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (AB267796), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (AB267796), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCIPFlow CytWBICC/IFIHC-P
Human
Expected
Not recommended
Not recommended
Tested
Not recommended
Expected
Mouse
Tested
Not recommended
Not recommended
Tested
Not recommended
Tested
Rat
Tested
Not recommended
Not recommended
Tested
Not recommended
Tested
Recombinant full length protein - Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species
Mouse, Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Species
Human
Dilution info
-
Notes

-

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Recombinant full length protein - Human, Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

This antibody is not recommended for human IHC-P

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

This antibody is not recommended for human IHC-P

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase (PubMed:10656250, PubMed:10677483, PubMed:20354142). Cleaves CHL1 (By similarity).

Alternative names

BACE, KIAA1149, BACE1, Beta-secretase 1, Aspartyl protease 2, Beta-site amyloid precursor protein cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2, ASP2, Asp 2, Beta-site APP cleaving enzyme 1

Recommended products

Rabbit Recombinant Monoclonal BACE1 antibody. Carrier free. Suitable for mIHC, WB, IHC-P and reacts with Mouse, Rat, Human, Recombinant full length protein - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR22802-233
Purification technique
Affinity purification Protein A
Specificity

This antibody is not recommended for human IHC-P.

This antibody shows very low cross-reactivity with BACE2.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab267796 is the carrier-free version of Anti-BACE1 antibody [EPR22802-233] ab263901.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

BACE1 also known as beta-site APP cleaving enzyme 1 or beta-secretase plays an important role in the cleavage of amyloid precursor protein (APP). This cleavage results in the production of amyloid-beta peptides which are associated with Alzheimer's disease. BACE1 is a membrane-bound aspartic protease and has a molecular weight of approximately 50100 Da. The enzyme expresses mostly in the brain's neurons and some secretory tissues.

Biological function summary

BACE1 initiates the amyloidogenic pathway of APP processing which involves amyloid-beta generation. BACE1 doesn't function alone but acts as part of a complex that aids in protein substrate recognition and processing. Its activity contributes to physiological processes like myelination and axonal guidance indicating its importance beyond amyloid production.

Pathways

BACE1 is integral to the amyloidogenic pathway which is important in Alzheimer's disease development. It interacts with proteins such as presenilin 1 a part of the gamma-secretase complex that further processes the amyloid-beta precursor. Furthermore BACE1 links to synaptic functions and neural signaling pathways highlighting its multifaceted roles.

Associated diseases and disorders

BACE1 holds significance in Alzheimer's disease due to its role in amyloid-beta peptide production. This connection has led to the development of BACE1 inhibitors as potential therapeutic agents. Additionally BACE1’s involvement in other neural functions ties it to cognitive impairments. It also relates to APP through its function in Alzheimer's suggesting targeted strategies for treatment could involve modulating BACE1 activity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796), expandable thumbnail

    Western blot - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1-2:26 seconds;
    Lanes 3-6:10 seconds.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 17425515).

    Anti-BACE1 antibody [EPR22802-233] ab263901 was shown to specifically react with BACE1 in wild-type HAP1 cells as signal was lost in BACE1 knockout cells. Wild-type and BACE1 knockout samples were subjected to SDS-PAGE. Anti-BACE1 antibody [EPR22802-233] ab263901 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BACE1 antibody [EPR22802-233] ab263901).

    All lanes: Western blot - Anti-BACE1 antibody [EPR22802-233] (Anti-BACE1 antibody [EPR22802-233] ab263901) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: BACE1 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: Mouse brain lysate at 20 µg

    Lane 5: Human brain lysate at 20 µg

    Lane 6: Rat hippocampus lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 56 kDa

    Observed band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796)

    Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling BACE1 with Anti-BACE1 antibody [EPR22802-233] ab263901 at 1/4000 dilution (0.19 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mossy fibers in the hilar region of the dentate gyrus. The section was incubated with Anti-BACE1 antibody [EPR22802-233] ab263901 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BACE1 antibody [EPR22802-233] ab263901).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796)

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling BACE1 with Anti-BACE1 antibody [EPR22802-233] ab263901 at 1/4000 dilution (0.19 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mossy fibers in the hilar region of the dentate gyrus. The section was incubated with Anti-BACE1 antibody [EPR22802-233] ab263901 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101)..

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BACE1 antibody [EPR22802-233] ab263901).

  • Multiplex immunohistochemistry - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796), expandable thumbnail

    Multiplex immunohistochemistry - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BACE1 antibody [EPR22802-233] ab263901).

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat hippocampus tissue staining SYNPR with Anti-SYNPR antibody [EPR28139-74] ab314136 at a 1:8000 (0.06 ug/ml) dilution, BACE1 with Anti-BACE1 antibody [EPR22802-233] ab263901 at 1:4000 (0.193 ug/ml) dilution and 5HT6 Receptor with Anti-5HT6 Receptor antibody [EPR26400-50] ab315380 at a 1:1000 (0.504 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

    Panel A: merged staining of anti-SYNPR (green; Opal™520), anti-BACE1 (magenta;Opal™570) and anti-5HT6 Receptor (yellow;Opal™690) on rat hippocampus.
    Panel B: anti-SYNPR staining the mossy fibers in rat hippocampus.
    Panel C: anti-BACE1 staining the mossy fibers in rat hippocampus.
    Panel D: anti-5HT6 Receptor staining the pyramidal neurons in rat hippocampus.
    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-SYNPR antibody [EPR28139-74] ab314136, Anti-BACE1 antibody [EPR22802-233] ab263901 and Anti-5HT6 Receptor antibody [EPR26400-50] ab315380 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796), expandable thumbnail

    Multiplex immunohistochemistry - Anti-BACE1 antibody [EPR22802-233] - BSA and Azide free (ab267796)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BACE1 antibody [EPR22802-233] ab263901).

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse hippocampus tissue staining SYNPR with Anti-SYNPR antibody [EPR28139-74] ab314136 at a 1:8000 (0.06 ug/ml) dilution, BACE1 with Anti-BACE1 antibody [EPR22802-233] ab263901 at 1:4000 (0.193 ug/ml) dilution and 5HT6 Receptor with Anti-5HT6 Receptor antibody [EPR26400-50] ab315380 at a 1:1000 (0.504 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

    Panel A: merged staining of anti-SYNPR (green; Opal™520), anti-BACE1 (magenta;Opal™570) and anti-5HT6 Receptor (yellow;Opal™690) on mouse hippocampus.
    Panel B: anti-SYNPR staining the mossy fibers in mouse hippocampus.
    Panel C: anti-BACE1 staining the mossy fibers in mouse hippocampus.
    Panel D: anti-5HT6 Receptor staining the pyramidal neurons in mouse hippocampus.
    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-SYNPR antibody [EPR28139-74] ab314136, Anti-BACE1 antibody [EPR22802-233] ab263901 and Anti-5HT6 Receptor antibody [EPR26400-50] ab315380 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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