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Anti-BACE1 antibody [EPR3956] (ab108394) is a rabbit monoclonal antibody that is used to detect BACE1 in Western Blot, IP. Suitable for Human, Mouse, Rat samples.



- Specificity confirmed with BACE1 knockout cell line validation


Images

Western blot - Anti-BACE1 antibody [EPR3956] (AB108394), expandable thumbnail
  • Western blot - Anti-BACE1 antibody [EPR3956] (AB108394), expandable thumbnail
  • Immunoprecipitation - Anti-BACE1 antibody [EPR3956] (AB108394), expandable thumbnail
  • Western blot - Anti-BACE1 antibody [EPR3956] (AB108394), expandable thumbnail
  • Western blot - Anti-BACE1 antibody [EPR3956] (AB108394), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Tested
Not recommended
Tested
Not recommended
Not recommended
Mouse
Expected
Not recommended
Tested
Not recommended
Not recommended
Rat
Expected
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

2 products for Alternative Version

Target data

Function

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase (PubMed:10656250, PubMed:10677483, PubMed:20354142). Cleaves CHL1 (By similarity).

Alternative names

BACE, KIAA1149, BACE1, Beta-secretase 1, Aspartyl protease 2, Beta-site amyloid precursor protein cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2, ASP2, Asp 2, Beta-site APP cleaving enzyme 1

Recommended products

Anti-BACE1 antibody [EPR3956] (ab108394) is a rabbit monoclonal antibody that is used to detect BACE1 in Western Blot, IP. Suitable for Human, Mouse, Rat samples.



- Specificity confirmed with BACE1 knockout cell line validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3956
Purification technique
Affinity purification Protein A
Specificity

This antibody shows very low cross-reactivity with BACE2.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

What is this antibody validated in?


Anti-BACE1 antibody [EPR3956] (ab108394) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), in Human, Mouse, Rat samples.

What is the molecular weight of BACE1?


Anti-BACE1 [EPR3956] (ab108394) specifically detects a band for BACE1 (UniProt: P56817) at a molecular weight of 56kDa.

Recommended positive controls


WB: SH-SY5Y cell lysate and human fetal, mouse and rat brain tissue lysates.IP: Human fetal brain tissue lysate.

Trusted by the scientific community


Anti-BACE1 [EPR3956] (ab108394) was first used in a scientific publication in 2011 and has been cited over 60 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed


The specificity of Anti-BACE1 antibody [EPR3956] (ab108394) has been confirmed by Western blot testing in BACE1 Knockout HAP1 cells.



Other related products


We have a range of other formats of antibody clone [EPR3956] also available for your convenience:
ab108394, Carrier free - Anti-BACE1 antibody [EPR3956] - BSA and Azide free ab237595



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

BACE1 also known as beta-site APP cleaving enzyme 1 or beta-secretase plays an important role in the cleavage of amyloid precursor protein (APP). This cleavage results in the production of amyloid-beta peptides which are associated with Alzheimer's disease. BACE1 is a membrane-bound aspartic protease and has a molecular weight of approximately 50100 Da. The enzyme expresses mostly in the brain's neurons and some secretory tissues.

Biological function summary

BACE1 initiates the amyloidogenic pathway of APP processing which involves amyloid-beta generation. BACE1 doesn't function alone but acts as part of a complex that aids in protein substrate recognition and processing. Its activity contributes to physiological processes like myelination and axonal guidance indicating its importance beyond amyloid production.

Pathways

BACE1 is integral to the amyloidogenic pathway which is important in Alzheimer's disease development. It interacts with proteins such as presenilin 1 a part of the gamma-secretase complex that further processes the amyloid-beta precursor. Furthermore BACE1 links to synaptic functions and neural signaling pathways highlighting its multifaceted roles.

Associated diseases and disorders

BACE1 holds significance in Alzheimer's disease due to its role in amyloid-beta peptide production. This connection has led to the development of BACE1 inhibitors as potential therapeutic agents. Additionally BACE1’s involvement in other neural functions ties it to cognitive impairments. It also relates to APP through its function in Alzheimer's suggesting targeted strategies for treatment could involve modulating BACE1 activity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-BACE1 antibody [EPR3956] (ab108394), expandable thumbnail

    Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: BACE1 knockout HAP1 whole cell lysate (20 μg)
    Lane 3: SHSY5Y whole cell lysate (20 μg)
    Lane 4: Human brain whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab108394 observed at 70 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab108394 was shown to recognize BACE1 in wild type cells as signal was lost at the expected MW in BACE1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BACE1 knockout samples were subjected to SDS-PAGE. ab108394 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)

    Predicted band size: 56 kDa

  • Western blot - Anti-BACE1 antibody [EPR3956] (ab108394), expandable thumbnail

    Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-BACE1 antibody [EPR3956] (ab108394) at 1/10000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 56 kDa

    Observed band size: 70 kDa

  • Immunoprecipitation - Anti-BACE1 antibody [EPR3956] (ab108394), expandable thumbnail

    Immunoprecipitation - Anti-BACE1 antibody [EPR3956] (ab108394)

    ab108394 (purified) at 1/40 immunoprecipitating BACE1 in human fetal brain whole tissue lysate.

    Lane 1 (input): human fetal brain whole tissue lysate (10μg)

    Lane 2 (+): ab108394 + human fetal brain whole tissue lysate (10μg).

    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab108394 in human fetal brain whole tissue lysate.

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1500 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-BACE1 antibody [EPR3956] (ab108394)

    Predicted band size: 39 kDa, 56 kDa

    Observed band size: 42 kDa, 70 kDa

  • Western blot - Anti-BACE1 antibody [EPR3956] (ab108394), expandable thumbnail

    Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-BACE1 antibody [EPR3956] (ab108394) at 1/10000 dilution

    All lanes: Human fetal brain tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 56 kDa

    Observed band size: 70 kDa

  • Western blot - Anti-BACE1 antibody [EPR3956] (ab108394), expandable thumbnail

    Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)

    All lanes: Western blot - Anti-BACE1 antibody [EPR3956] (ab108394) at 1/1000 dilution

    Lane 1: Fetal brain lysate at 10 µg

    Lane 2: SH-SY5Y lysate at 10 µg

    Lane 3: Mouse brain lysate at 10 µg

    Lane 4: Rat brain lysate at 10 µg

    Predicted band size: 56 kDa

  • Western blot - Anti-BACE1 antibody [EPR3956] (ab108394), expandable thumbnail

    Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)

    Western blot: Anti-BACE1 antibody [EPR3956] (ab108394) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab108394 was shown to bind specifically to BACE1. A band was observed at 56 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in BACE1 knockout cell line. To generate this image, wild-type and BACE1 knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-BACE1 antibody [EPR3956] (ab108394) at 1/1000 dilution

    Lane 1: Wild-type U-87 MG cell lysate at 20 µg

    Lane 2: Western blot - Human BACE1 knockout U-87 MG cell line (ab306623)

    Lane 2: BACE1 knockout U-87 MG cell lysate at 20 µg

    Lane 3: Wild-type SH-SY5Y cell lysate at 20 µg

    Lane 4: BACE1 knockout SH-SY5Y ab280078 cell lysate at 20 µg

    Secondary

    Lanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

    Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 56 kDa

  • Western blot - Anti-BACE1 antibody [EPR3956] (ab108394), expandable thumbnail

    Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)

    False colour image of Western blot: Anti-BACE1 antibody [EPR3956] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108394 was shown to bind specifically to BACE1. A band was observed at 60/70 kDa in wild-type SH-SY5Y cell lysates with no signal observed at this size in Bace1 knockout cell line ab280078 (knockout cell lysate ab280137).

    To generate this image, wild-type and Bace1 knockout SH-SY5Y cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    Lanes 1 - 3: Western blot - Anti-BACE1 antibody [EPR3956] (ab108394) at 1/1000 dilution

    Lanes 1 - 3: Western blot - Anti-BACE1 antibody [EPR3956] - BSA and Azide free (Anti-BACE1 antibody [EPR3956] - BSA and Azide free ab237595) at 1/1000 dilution

    Lane 1: Wild-type SH-SY5Y cell lysate at 20 µg

    Lane 2: Bace1 knockout SH-SY5Y cell lysate at 20 µg

    Lane 2: Western blot - Human BACE1 knockout SH-SY5Y cell line (ab280078)

    Lane 2: Western blot - Human BACE1 knockout SH-SY5Y cell lysate (ab280137)

    Lane 3: HAP1 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680R at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 70 kDa, 60 kDa

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