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Knockout Tested Rabbit Recombinant Monoclonal BACE1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-BACE1 antibody [EPR3956] - BSA and Azide free (AB237595), expandable thumbnail
  • Immunoprecipitation - Anti-BACE1 antibody [EPR3956] - BSA and Azide free (AB237595), expandable thumbnail
  • Western blot - Anti-BACE1 antibody [EPR3956] - BSA and Azide free (AB237595), expandable thumbnail
  • Western blot - Anti-BACE1 antibody [EPR3956] - BSA and Azide free (AB237595), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Tested
Not recommended
Tested
Not recommended
Not recommended
Mouse
Expected
Not recommended
Tested
Not recommended
Not recommended
Rat
Expected
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

2 products for Alternative Version

Target data

Function

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase (PubMed:10656250, PubMed:10677483, PubMed:20354142). Cleaves CHL1 (By similarity).

Alternative names

BACE, KIAA1149, BACE1, Beta-secretase 1, Aspartyl protease 2, Beta-site amyloid precursor protein cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2, ASP2, Asp 2, Beta-site APP cleaving enzyme 1

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal BACE1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR3956
Purification technique
Affinity purification Protein A
Specificity

This antibody shows very low cross-reactivity with BACE2.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab237595 is the carrier-free version of Anti-BACE1 antibody [EPR3956] ab108394.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

BACE1 also known as beta-site APP cleaving enzyme 1 or beta-secretase plays an important role in the cleavage of amyloid precursor protein (APP). This cleavage results in the production of amyloid-beta peptides which are associated with Alzheimer's disease. BACE1 is a membrane-bound aspartic protease and has a molecular weight of approximately 50100 Da. The enzyme expresses mostly in the brain's neurons and some secretory tissues.

Biological function summary

BACE1 initiates the amyloidogenic pathway of APP processing which involves amyloid-beta generation. BACE1 doesn't function alone but acts as part of a complex that aids in protein substrate recognition and processing. Its activity contributes to physiological processes like myelination and axonal guidance indicating its importance beyond amyloid production.

Pathways

BACE1 is integral to the amyloidogenic pathway which is important in Alzheimer's disease development. It interacts with proteins such as presenilin 1 a part of the gamma-secretase complex that further processes the amyloid-beta precursor. Furthermore BACE1 links to synaptic functions and neural signaling pathways highlighting its multifaceted roles.

Associated diseases and disorders

BACE1 holds significance in Alzheimer's disease due to its role in amyloid-beta peptide production. This connection has led to the development of BACE1 inhibitors as potential therapeutic agents. Additionally BACE1’s involvement in other neural functions ties it to cognitive impairments. It also relates to APP through its function in Alzheimer's suggesting targeted strategies for treatment could involve modulating BACE1 activity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-BACE1 antibody [EPR3956] - BSA and Azide free (ab237595), expandable thumbnail

    Western blot - Anti-BACE1 antibody [EPR3956] - BSA and Azide free (ab237595)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: BACE1 knockout HAP1 whole cell lysate (20 μg)
    Lane 3: SH-SY5Y whole cell lysate (20 μg)
    Lane 4: Human brain whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-BACE1 antibody [EPR3956] ab108394 observed at 70 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    Anti-BACE1 antibody [EPR3956] ab108394 was shown to recognize BACE1 in wild type cells as signal was lost at the expected MW in BACE1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BACE1 knockout samples were subjected to SDS-PAGE. Anti-BACE1 antibody [EPR3956] ab108394 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BACE1 antibody [EPR3956] ab108394).

    All lanes: Western blot - Anti-BACE1 antibody [EPR3956] (Anti-BACE1 antibody [EPR3956] ab108394)

    Predicted band size: 56 kDa

  • Immunoprecipitation - Anti-BACE1 antibody [EPR3956] - BSA and Azide free (ab237595), expandable thumbnail

    Immunoprecipitation - Anti-BACE1 antibody [EPR3956] - BSA and Azide free (ab237595)

    Anti-BACE1 antibody [EPR3956] ab108394 (purified) at 1/40 immunoprecipitating BACE1 in human fetal brain whole tissue lysate.

    Lane 1 (input): human fetal brain whole tissue lysate (10µg)

    Lane 2 (+): Anti-BACE1 antibody [EPR3956] ab108394 + human fetal brain whole tissue lysate (10µg).

    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-BACE1 antibody [EPR3956] ab108394 in human fetal brain whole tissue lysate.

    For western blotting, VeriBlot for IP Detection Reagent (HRP) ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BACE1 antibody [EPR3956] ab108394).

    All lanes: Immunoprecipitation - Anti-BACE1 antibody [EPR3956] (Anti-BACE1 antibody [EPR3956] ab108394)

    Predicted band size: 39 kDa, 56 kDa

    Observed band size: 42 kDa, 70 kDa

  • Western blot - Anti-BACE1 antibody [EPR3956] - BSA and Azide free (ab237595), expandable thumbnail

    Western blot - Anti-BACE1 antibody [EPR3956] - BSA and Azide free (ab237595)

    This data was developed using Anti-BACE1 antibody [EPR3956] ab108394, the same antibody clone in a different buffer formulation.

    Western blot: Anti-BACE1 antibody [EPR3956] (Anti-BACE1 antibody [EPR3956] ab108394) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-BACE1 antibody [EPR3956] ab108394 was shown to bind specifically to BACE1. A band was observed at 56 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in BACE1 knockout cell line. To generate this image, wild-type and BACE1 knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-BACE1 antibody [EPR3956] (Anti-BACE1 antibody [EPR3956] ab108394) at 1/1000 dilution

    Lane 1: Wild-type U-87 MG cell lysate at 20 µg

    Lane 2: Western blot - Human BACE1 knockout U-87 MG cell line (ab306623)

    Lane 2: BACE1 knockout U-87 MG cell lysate at 20 µg

    Lane 3: Wild-type SH-SY5Y cell lysate at 20 µg

    Lane 4: BACE1 knockout SH-SY5Y ab280078 cell lysate at 20 µg

    Secondary

    Lanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

    Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 56 kDa

  • Western blot - Anti-BACE1 antibody [EPR3956] - BSA and Azide free (ab237595), expandable thumbnail

    Western blot - Anti-BACE1 antibody [EPR3956] - BSA and Azide free (ab237595)

    False colour image of Western blot: Anti-BACE1 antibody [EPR3956] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-BACE1 antibody [EPR3956] ab108394 was shown to bind specifically to BACE1. A band was observed at 60/70 kDa in wild-type SH-SY5Y cell lysates with no signal observed at this size in Bace1 knockout cell line ab280078 (knockout cell lysate ab280137).

    To generate this image, wild-type and Bace1 knockout SH-SY5Y cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation (Anti-BACE1 antibody [EPR3956] ab108394)

    Lanes 1 - 3: Western blot - Anti-BACE1 antibody [EPR3956] (Anti-BACE1 antibody [EPR3956] ab108394) at 1/1000 dilution

    Lanes 1 - 3: Western blot - Anti-BACE1 antibody [EPR3956] - BSA and Azide free (ab237595) at 1/1000 dilution

    Lane 1: Wild-type SH-SY5Y cell lysate at 20 µg

    Lane 2: Bace1 knockout SH-SY5Y cell lysate at 20 µg

    Lane 2: Western blot - Human BACE1 knockout SH-SY5Y cell line (ab280078)

    Lane 2: Western blot - Human BACE1 knockout SH-SY5Y cell lysate (ab280137)

    Lane 3: HAP1 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680R at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 70 kDa, 60 kDa

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Product protocols

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