Anti-BACE2 antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-BACE2 antibody (AB5670)

Immunocytochemistry/Immunofluorescence analysis of BACE2 (green) showing staining in the nucleus and cytoplasm of A549 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5670 in 3% BSA-PBS at a dilution of 1 : 100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

• WB

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Western blot - Anti-BACE2 antibody (AB5670)

All lanes:

Western blot - Anti-BACE2 antibody (ab5670) at 1/500 dilution

Lane 1:

Mouse spleen cell lysate at 25 µg

Lane 2:

SH-SY5Y cell lysate at 25 µg

Lane 3:

HL60 cell lysate at 25 µg

Lane 4:

HeLa cell lysate at 25 µg

Predicted band size: 56 kDa

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