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AB327225

Anti-BACH1 Antibody

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Rabbit Polyclonal BACH1.3 antibody. Suitable for IHC-P, ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Transcription regulator protein BACH1.

View Alternative Names

Transcription regulator protein BACH1, BTB and CNC homolog 1, HA2303, BACH1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1 Antibody (AB327225)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1 Antibody (AB327225)

Immunohistochemical staining of human tonsil using ab327225 at 1 : 200–1 : 500 dilution, showing moderate nuclear positivity in germinal center cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1 Antibody (AB327225)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1 Antibody (AB327225)

Immunohistochemical staining of human skin using ab327225 at 1 : 200–1 : 500 dilution, showing strong cytoplasmic positivity in squamous epithelial cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1 Antibody (AB327225)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1 Antibody (AB327225)

Immunohistochemical staining of human testis using ab327225 at 1 : 200–1 : 500 dilution, showing moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts.

Immunocytochemistry/ Immunofluorescence - Anti-BACH1 Antibody (AB327225)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BACH1 Antibody (AB327225)

Immunofluorescent staining of human cell line A-431 using ab327225 at 0.25–2 µg/ml, showing localization to nucleoplasm.

ChIP - Anti-BACH1 Antibody (AB327225)
  • ChIP

Supplier Data

ChIP - Anti-BACH1 Antibody (AB327225)

ChIP-Exo-Seq composite graph for ab327225 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1 Antibody (AB327225)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1 Antibody (AB327225)

Immunohistochemical staining of human pancreas using ab327225 at 1 : 200–1 : 500 dilution, showing no positivity in exocrine glandular cells.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ChIP, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Transcription regulator protein BACH1.

O14867

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator that acts as a repressor or activator, depending on the context. Binds to NF-E2 DNA binding sites. Plays important roles in coordinating transcription activation and repression by MAFK (By similarity). Together with MAF, represses the transcription of genes under the control of the NFE2L2 oxidative stress pathway (PubMed : 24035498).
See full target information Transcription regulator protein BACH1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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