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AB264133

Anti-BACH1/BRIP1 antibody

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Rabbit Polyclonal BACH1/BRIP1 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human BRIP1 aa 1200 to C-terminus.

View Alternative Names

BACH1, FANCJ, BRIP1, Fanconi anemia group J protein, BRCA1-associated C-terminal helicase 1, BRCA1-interacting protein C-terminal helicase 1, DNA 5'-3' helicase FANCJ, BRCA1-interacting protein 1

2 Images
Immunoprecipitation - Anti-BACH1/BRIP1 antibody (AB264133)
  • IP

Supplier Data

Immunoprecipitation - Anti-BACH1/BRIP1 antibody (AB264133)

BACH1/BRIP1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264133 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab264133 at 1 μg/ml.

Lane 1 : ab264133 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 10 secs.

All lanes:

Immunoprecipitation - Anti-BACH1/BRIP1 antibody (ab264133)

Predicted band size: 141 kDa

false

Western blot - Anti-BACH1/BRIP1 antibody (AB264133)
  • WB

Supplier Data

Western blot - Anti-BACH1/BRIP1 antibody (AB264133)

4-8% SDS-PAGE

All lanes:

Western blot - Anti-BACH1/BRIP1 antibody (ab264133) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T whole cell lysate at 50 µg

Predicted band size: 141 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human BRIP1 aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BX63

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-4 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BACH1 also known as BRIP1 or FANCJ is a DNA helicase with a molecular weight of approximately 130 kDa. This protein plays an important role in unwinding DNA which is essential for replication and repair processes. BACH1 is expressed in the nucleus of cells especially in tissues with high proliferative rates like germ cells and certain stem cells. It possesses a unique ability to interact with other proteins engaging in DNA repair and maintenance activities.
Biological function summary

BACH1 functions as a DNA repair protein integral to maintaining genomic stability. It forms part of the BRCA1 complex facilitating DNA damage repair by interacting with key partners such as BRCA1 and other related proteins. This protein ensures precise repair of double-stranded DNA breaks preventing mutations and maintaining cell viability. Its role makes it indispensable in cell cycle checkpoints and maintenance of chromosome integrity.

Pathways

BACH1 is essential to the DNA damage response and homologous recombination repair pathways. It participates in recruiting other repair proteins like BRCA1 to sites of damage enabling effective repair. BACH1's interactions play an important role in the Fanconi anemia pathway along with proteins like RAD51 contributing to crosslink repair processes. These pathways ensure cells properly react to DNA damage reducing mutagenesis risk.

BACH1 mutations link to breast and ovarian cancers due to its critical role in DNA repair. These mutations often disrupt its interaction with BRCA1 relating BACH1 to hereditary cancer syndromes. In addition deficiencies in its function connect to Fanconi anemia a genetic disorder marked by bone marrow failure and cancer proneness. The protein’s malfunction in these conditions highlights its importance in protecting against genomic instability and disease development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of chromosomal stability (PubMed : 11301010, PubMed : 14983014, PubMed : 16116421, PubMed : 16153896, PubMed : 17596542, PubMed : 36608669). Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination (PubMed : 14983014, PubMed : 16153896). Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1 (PubMed : 14983014, PubMed : 16153896). Involved in the repair of abasic sites at replication forks by promoting the degradation of DNA-protein cross-links : acts by catalyzing unfolding of HMCES DNA-protein cross-link via its helicase activity, exposing the underlying DNA and enabling cleavage of the DNA-protein adduct by the SPRTN metalloprotease (PubMed : 16116421, PubMed : 36608669). Can unwind RNA : DNA substrates (PubMed : 14983014). Unwinds G-quadruplex DNA; unwinding requires a 5'-single stranded tail (PubMed : 18426915, PubMed : 20639400).
See full target information BRIP1
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