AB49657

Anti-BACH1/BRIP1 antibody

Name: Anti-BACH1/BRIP1 antibody (ab49657) | Abcam
Brand: Abcam Limited
SKU: AB49657

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(11 Publications)

Rabbit Polyclonal BACH1/BRIP1 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human BRIP1 aa 1200 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

BACH1, FANCJ, BRIP1, Fanconi anemia group J protein, BRCA1-associated C-terminal helicase 1, BRCA1-interacting protein C-terminal helicase 1, DNA 5'-3' helicase FANCJ, BRCA1-interacting protein 1

3 Images

Supplier Data

Immunocytochemistry - Anti-BACH1/BRIP1 antibody (AB49657)

Immunocytochemistry analysis of MCF-7 cells labeling BACH1/BRIP1 with ab49657 at 20 μg/mL followed by Goat Anti-Rabbit IgG (whole molecule)-FITC secondary antibody at 1/100. Cells were fixed and permeabilized with 4% paraformaldehyde. Panel C : cells were counterstained with DAPI (blue) to stain nuclei (Panel A : without DAPI, Panel B : only DAPI).

Supplier Data

Western blot - Anti-BACH1/BRIP1 antibody (AB49657)

All lanes:

Western blot - Anti-BACH1/BRIP1 antibody (ab49657) at 1/2000 dilution

All lanes:

MCF-7 cell lysate

Secondary

All lanes:

HRP conjugated Anti-Rabbit IgG antibody

Predicted band size: 141 kDa

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Lab

Western blot - Anti-BACH1/BRIP1 antibody (AB49657)

Western blot : Rabbit Polyclonal to BACH1/BRIP1 ab49657 staining at 1/2000 dilution, shown in green; Mouse anti alpha Tubulin (ab7291) loading control staining at 1/20,000 dilution, shown in magenta.

A band was observed at 151 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in BRIP1 knockout MCF7 cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-BACH1/BRIP1 antibody (ab49657) at 1/2000 dilution

Lane 1:

Wild-type MCF7 at 20 µg

Lane 2:

BRIP1 knockout MCF7 at 20 µg

Lane 2:

Western blot - Human BRIP1 knockout MCF7 cell line (ab286257) at 20 µg

Lane 3:

MOLT-4 at 20 µg

Lane 4:

Jurkat at 20 µg

Lane 5:

Raji at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 141 kDa

Observed band size: 151 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, WB

applications

Immunogen

Synthetic Peptide within Human BRIP1 aa 1200 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9BX63

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10-20 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL for 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BACH1 also known as BRIP1 or FANCJ is a DNA helicase with a molecular weight of approximately 130 kDa. This protein plays an important role in unwinding DNA which is essential for replication and repair processes. BACH1 is expressed in the nucleus of cells especially in tissues with high proliferative rates like germ cells and certain stem cells. It possesses a unique ability to interact with other proteins engaging in DNA repair and maintenance activities.

Biological function summary

BACH1 functions as a DNA repair protein integral to maintaining genomic stability. It forms part of the BRCA1 complex facilitating DNA damage repair by interacting with key partners such as BRCA1 and other related proteins. This protein ensures precise repair of double-stranded DNA breaks preventing mutations and maintaining cell viability. Its role makes it indispensable in cell cycle checkpoints and maintenance of chromosome integrity.

Pathways

BACH1 is essential to the DNA damage response and homologous recombination repair pathways. It participates in recruiting other repair proteins like BRCA1 to sites of damage enabling effective repair. BACH1's interactions play an important role in the Fanconi anemia pathway along with proteins like RAD51 contributing to crosslink repair processes. These pathways ensure cells properly react to DNA damage reducing mutagenesis risk.

BACH1 mutations link to breast and ovarian cancers due to its critical role in DNA repair. These mutations often disrupt its interaction with BRCA1 relating BACH1 to hereditary cancer syndromes. In addition deficiencies in its function connect to Fanconi anemia a genetic disorder marked by bone marrow failure and cancer proneness. The protein’s malfunction in these conditions highlights its importance in protecting against genomic instability and disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of chromosomal stability (PubMed : 11301010, PubMed : 14983014, PubMed : 16116421, PubMed : 16153896, PubMed : 17596542, PubMed : 36608669). Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination (PubMed : 14983014, PubMed : 16153896). Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1 (PubMed : 14983014, PubMed : 16153896). Involved in the repair of abasic sites at replication forks by promoting the degradation of DNA-protein cross-links : acts by catalyzing unfolding of HMCES DNA-protein cross-link via its helicase activity, exposing the underlying DNA and enabling cleavage of the DNA-protein adduct by the SPRTN metalloprotease (PubMed : 16116421, PubMed : 36608669). Can unwind RNA : DNA substrates (PubMed : 14983014). Unwinds G-quadruplex DNA; unwinding requires a 5'-single stranded tail (PubMed : 18426915, PubMed : 20639400).

See full target information BRIP1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Drug development research 85:e22256 PubMed39285641

2024

m6A-activated BACH1 exacerbates ferroptosis by epigenetic suppression HSPB1 in severe acute pancreatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Fawei Zhou,Dezhong Li,Chang Liu,Can Li,Kaili Li,Lu Shi,Fachun Zhou

Human molecular genetics 31:4241-4254 PubMed35904444

2022

Identification of new RAD51D-regulating microRNAs that also emerge as potent inhibitors of the Fanconi anemia/homologous recombination pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Hater,Katharina M Iwaniuk,Carina Leifeld,Pia Grüten,Constanze Wiek,Katharina Raba,Fan Zhang,Johannes C Fischer,Paul R Andreassen,Helmut Hanenberg,Hans-Ingo Trompeter

Frontiers in pharmacology 13:857932 PubMed35462905

2022

Bushen Culuan Decoction Ameliorates Premature Ovarian Insufficiency by Acting on the Nrf2/ARE Signaling Pathway to Alleviate Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yanxia Chen,Xiaodi Fan,Kun Ma,Kaili Wang,Caidie Tian,Min Li,Linjuan Gong

Experimental and therapeutic medicine 21:516 PubMed33815589

2021

Expression of nuclear factor erythroid-2-related factor 2, broad complex-tramtrack-bric a brac and Cap'n'collar homology 1 and γ-glutamic acid cysteine synthase in peripheral blood of patients with chronic obstructive pulmonary disease and its clinical significance.

Applications

Unspecified application

Species

Unspecified reactive species

Dongrong Li,Ding Sun,Yehan Zhu

BMC ophthalmology 21:104 PubMed33632168

2021

Haem relieves hyperoxia-mediated inhibition of HMEC-1 cell proliferation, migration and angiogenesis by inhibiting BACH1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Jian,Yang Mei,Chen Xing,Yuan Rongdi

Molecular and cellular biology 40: PubMed32989015

2020

Loss of Mitochondrial Localization of Human FANCG Causes Defective FANCJ Helicase.

Applications

Unspecified application

Species

Unspecified reactive species

Jagadeesh Chandra Bose K,Bishwajit Singh Kapoor,Kamal Mandal,Shubhrima Ghosh,Raveendra B Mokhamatam,Sunil K Manna,Sudit S Mukhopadhyay

The EMBO journal 39:e104133 PubMed32347575

2020

BGL3 lncRNA mediates retention of the BRCA1/BARD1 complex at DNA damage sites.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaohua Hu,Shaojie Mi,Ting Zhao,Changmin Peng,Yihan Peng,Lulu Chen,Wenge Zhu,Yi Yao,Qibin Song,Xiangpan Li,Xinzhi Li,Chenxi Jia,Huadong Pei

The Kaohsiung journal of medical sciences 35:417-424 PubMed30977589

2019

Protective effects of HO-1 pathway on lung injury subsequent to limb ischemia reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Yan Li,Chun-Yan Liu,Mei Liu,Ke-Yu Sun

EBioMedicine 39:69-82 PubMed30545799

2018

Inhibition of AZIN2-sv induces neovascularization and improves prognosis after myocardial infarction by blocking ubiquitin-dependent talin1 degradation and activating the Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinzhong Li,Yili Sun,Senlin Huang,Yanmei Chen,Xiaoqiang Chen,Mengsha Li,Xiaoyun Si,Xiang He,Hao Zheng,Lintao Zhong,Yang Yang,Wangjun Liao,Yulin Liao,Guojun Chen,Jianping Bin

Nucleic acids research 45:8886-8900 PubMed28911102

2017

FANCJ helicase controls the balance between short- and long-tract gene conversions between sister chromatids.

Applications

Unspecified application

Species

Unspecified reactive species

Sarmi Nath,Kumar Somyajit,Anup Mishra,Ralph Scully,Ganesh Nagaraju

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-BACH1/BRIP1 antibody

Immunocytochemistry - Anti-BACH1/BRIP1 antibody (AB49657)

Western blot - Anti-BACH1/BRIP1 antibody (AB49657)

Western blot - Anti-BACH1/BRIP1 antibody (AB49657)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise