Rabbit Polyclonal BACH1/BRIP1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BRIP1 aa 50-300.
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Publications
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- Endocrine connections 7:65-772017overexpression is correlated with clinical features and survival outcome of luminal breast cancer subtypes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIshita Gupta,Allal Ouhtit,Adil Al-Ajmi,Syed Gauhar A Rizvi,Hamad Al-Riyami,Marwa Al-Riyami,Yahya TamimiPubMed 29138235
- Nucleic acids research 44:6803-162016FANCJ is essential to maintain microsatellite structure genome-wide during replication stress.
- BMC cancer 14:2462014Gene expression profiling reveals activation of the FA/BRCA pathway in advanced squamous cervical cancer with intrinsic resistance and therapy failure.Applications:IHCReactive species:Unspecified reactive speciesOvidiu Balacescu,Loredana Balacescu,Oana Tudoran,Nicolae Todor,Meda Rus,Rares Buiga,Sergiu Susman,Bogdan Fetica,Laura Pop,Laura Maja,Simona Visan,Claudia Ordeanu,Ioana Berindan-Neagoe,Viorica NagyPubMed 24708616
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human BRIP1 aa 50-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BX63
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of chromosomal stability (PubMed:11301010, PubMed:14983014, PubMed:16116421, PubMed:16153896, PubMed:17596542, PubMed:36608669). Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination (PubMed:14983014, PubMed:16153896). Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1 (PubMed:14983014, PubMed:16153896). Involved in the repair of abasic sites at replication forks by promoting the degradation of DNA-protein cross-links: acts by catalyzing unfolding of HMCES DNA-protein cross-link via its helicase activity, exposing the underlying DNA and enabling cleavage of the DNA-protein adduct by the SPRTN metalloprotease (PubMed:16116421, PubMed:36608669). Can unwind RNA:DNA substrates (PubMed:14983014). Unwinds G-quadruplex DNA; unwinding requires a 5'-single stranded tail (PubMed:18426915, PubMed:20639400).
Alternative names
BACH1, FANCJ, BRIP1, Fanconi anemia group J protein, BRCA1-associated C-terminal helicase 1, BRCA1-interacting protein C-terminal helicase 1, DNA 5'-3' helicase FANCJ, BRCA1-interacting protein 1
Recommended products
Rabbit Polyclonal BACH1/BRIP1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BRIP1 aa 50-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human BRIP1 aa 50-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BX63
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
BACH1 also known as BRIP1 or FANCJ is a DNA helicase with a molecular weight of approximately 130 kDa. This protein plays an important role in unwinding DNA which is essential for replication and repair processes. BACH1 is expressed in the nucleus of cells especially in tissues with high proliferative rates like germ cells and certain stem cells. It possesses a unique ability to interact with other proteins engaging in DNA repair and maintenance activities.
- Biological function summary
BACH1 functions as a DNA repair protein integral to maintaining genomic stability. It forms part of the BRCA1 complex facilitating DNA damage repair by interacting with key partners such as BRCA1 and other related proteins. This protein ensures precise repair of double-stranded DNA breaks preventing mutations and maintaining cell viability. Its role makes it indispensable in cell cycle checkpoints and maintenance of chromosome integrity.
- Pathways
BACH1 is essential to the DNA damage response and homologous recombination repair pathways. It participates in recruiting other repair proteins like BRCA1 to sites of damage enabling effective repair. BACH1's interactions play an important role in the Fanconi anemia pathway along with proteins like RAD51 contributing to crosslink repair processes. These pathways ensure cells properly react to DNA damage reducing mutagenesis risk.
- Associated diseases and disorders
BACH1 mutations link to breast and ovarian cancers due to its critical role in DNA repair. These mutations often disrupt its interaction with BRCA1 relating BACH1 to hereditary cancer syndromes. In addition deficiencies in its function connect to Fanconi anemia a genetic disorder marked by bone marrow failure and cancer proneness. The protein’s malfunction in these conditions highlights its importance in protecting against genomic instability and disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-BACH1/BRIP1 antibody - N-terminal (ab151509)
5% SDS-PAGE.
All lanes: Western blot - Anti-BACH1/BRIP1 antibody - N-terminal (ab151509) at 1/1000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 141 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1/BRIP1 antibody - N-terminal (ab151509)
Immunohistochemical analysis of paraffin-embedded Human SAS xenograft tissue labeling BACH1/BRIP1 with ab151509 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-BACH1/BRIP1 antibody - N-terminal (ab151509)
Immunofluorescent analysis of paraformaldehyde-fixed 293T cells labeling BACH1/BRIP1 with ab151509 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33343.
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