JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB245549

Anti-BACH1.3 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal BACH1.3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Transcription regulator protein BACH1 aa 650-700.

View Alternative Names

Transcription regulator protein BACH1, BTB and CNC homolog 1, HA2303, BACH1

2 Images
Immunoprecipitation - Anti-BACH1.3 antibody (AB245549)
  • IP

Supplier Data

Immunoprecipitation - Anti-BACH1.3 antibody (AB245549)

BACH1.3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245549 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab245549 at 1 μg/ml.

Lane 1 : ab245549 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-BACH1.3 antibody (ab245549)

Predicted band size: 82 kDa

false

Western blot - Anti-BACH1.3 antibody (AB245549)
  • WB

Supplier Data

Western blot - Anti-BACH1.3 antibody (AB245549)

All lanes:

Western blot - Anti-BACH1.3 antibody (ab245549) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 82 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human Transcription regulator protein BACH1 aa 650-700. The exact immunogen used to generate this antibody is proprietary information.

O14867

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab245549 was affinity purified using an epitope specific to BACH1.3 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The BACH1.3 gene encodes a transcription regulator protein also known as BTB and CNC homology 1 basic leucine zipper transcription factor 1. The protein weighs approximately 105 kDa and you can find it in various tissues including the liver lungs and kidneys. It binds to the antioxidant response elements (ARE) in DNA regulating the transcription of genes involved in oxidative stress response. BACH1.3 functions by repressing gene activation through heterodimerization with small Maf proteins which helps maintain cellular redox balance.
Biological function summary

BACH1.3 modulates gene expression by forming complexes with small Maf proteins which are necessary for its regulatory activity. This protein plays a role in cellular oxidative stress management and heme metabolism. Through its function in stress response regulation BACH1.3 influences the expression of several genes such as heme oxygenase-1 (HO-1) important for detoxifying reactive oxygen species. By controlling these pathways BACH1.3 supports cell protection and adaptation to environmental oxidative stress.

Pathways

BACH1.3 participates in the oxidative stress response and heme degradation pathways. It partners with the KEAP1-NRF2 pathway where it acts as a negative regulator of NRF2 target genes by competing with NRF2 for binding to ARE sequences. This action impacts the transcription of antioxidant genes. BACH1.3 indirectly affects the expression of proteins like HO-1 and NAD(P)H:quinone oxidoreductase 1 (NQO1) moderating their protective effects in the cell.

Altered BACH1.3 function associates with cancer and cardiovascular diseases. Its involvement in improper oxidative stress response can lead to tumor progression and resistance to therapeutic agents in some cancers. Additionally in cardiovascular diseases BACH1.3 connects to abnormal arterial remodeling due to its regulation of genes involved in oxidative stress. This target interacts with proteins like KEAP1 and NRF2 modifying disease outcomes through changes in redox-sensitive signaling and gene expression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcriptional regulator that acts as a repressor or activator, depending on the context. Binds to NF-E2 DNA binding sites. Plays important roles in coordinating transcription activation and repression by MAFK (By similarity). Together with MAF, represses the transcription of genes under the control of the NFE2L2 oxidative stress pathway (PubMed : 24035498).
See full target information Transcription regulator protein BACH1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular immunology 19:516-526 PubMed34983952

2022

Enhancing the HSV-1-mediated antitumor immune response by suppressing Bach1.

Applications

Unspecified application

Species

Unspecified reactive species

Chaohu Pan,Qiaomei Cai,Xiaorong Li,Lili Li,Liping Yang,Yu Chen,Junxiao Liu,Wancheng Liu,Meiling Gao,Tianqi Sui,Xiaoyang Wang,Huiming Fan,Jiayin Ruan,Yueyue Shi,Saihua Chen,Lucy S Cheng,Jiayong Liu,Heng Yang,Genhong Cheng
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB245548

Anti-BACH1.3 antibody

Immunoprecipitation - Anti-BACH1.3 antibody (AB245548)

  • 0
  • 0
Primary Antibodies

AB128486

Anti-BACH1.3 antibody [OTI4E11]

Flow Cytometry (Intracellular) - Anti-BACH1.3 antibody [OTI4E11] (AB128486)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB186733

Anti-Argonaute-2 antibody [EPR10411]

KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Argonaute-2 antibody [EPR10411] (AB186733)

  • 0
  • 0
Primary Antibodies

AB26

Anti-p53 antibody [PAb 240]

KO Validated|Lab Essentials

Western blot - Anti-p53 antibody [PAb 240] (AB26)

  • 4
  • 56

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com