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AB252254

Anti-BACH2 antibody [7A4] - BSA and Azide free

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Rat Recombinant Monoclonal BACH2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.

View Alternative Names

Transcription regulator protein BACH2, BTB and CNC homolog 2, BACH2

1 Images
Western blot - Anti-BACH2 antibody [7A4] - BSA and Azide free (AB252254)
  • WB

Supplier Data

Western blot - Anti-BACH2 antibody [7A4] - BSA and Azide free (AB252254)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243148).

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

Exposure time :

Lane 1 : 3 minutes
Lanes 2-5 : 3 seconds
Lane 6 : 5 seconds

Negative control : THP-1 and HL-60 (PMID : 10949928).

The molecular weight observed is consistent with what has been described in the literature (PMID : 9755173).

All lanes:

Western blot - Anti-BACH2 antibody [7A4] (<a href='/en-us/products/primary-antibodies/bach2-antibody-7a4-ab243148'>ab243148</a>) at 1/2000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 2:

Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

Lane 3:

THP-1 (human monocytic leukemia monocyte), whole cell lysate at 20 µg

Lane 4:

HL-60 (human Acute Promyelocytic Leukemia promyeloblast), whole cell lysate at 20 µg

Lane 5:

NAMALWA (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

Lane 6:

WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

Predicted band size: 93 kDa

Observed band size: 130 kDa

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Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

7A4

Isotype

IgG2a

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab252254 is the carrier-free version of ab243148.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BACH2 also known as BTB and CNC homology 2 is a transcription factor with a molecular mass of around 95 kDa. It regulates the expression of certain genes by binding to specific DNA sequences. BACH2 maintains a zinc-finger domain and a BTB domain which are important for its binding and transcriptional repressor functions. The target expresses predominantly in immune cells especially in B cells and T cells playing a critical role in immune system regulation. It is also found in other tissues but its levels are comparatively higher in lymphoid organs.
Biological function summary

BACH2 plays an important role in the regulation of immune responses. It functions as a homodimer or forms heterodimers with other proteins such as BACH1 allowing it to modulate gene expression effectively. BACH2 regulates the balance between immune tolerance and activation by controlling the production of cytokines and the differentiation of T cells. This regulation is important for preventing autoimmune responses while enabling proper immune responses against infections.

Pathways

BACH2 participates actively in the oxidative stress response and pathways of lymphocyte development. In the oxidative stress pathway it interacts with proteins like KEAP1 and NRF2 to regulate antioxidant gene expression. In lymphocyte development it influences the recombination of immunoglobulin genes and supports the maturation of B cells into plasma cells. By doing so BACH2 ensures the proper functioning of the immune system working in conjunction with various transcriptional regulatory networks.

BACH2 has associations with several immune-related conditions. It is implicated in autoimmune diseases such as type 1 diabetes where improper regulation may lead to immune system attacks on healthy cells. Additionally changes in BACH2 expression levels have been linked to lymphoma a type of blood cancer involving the lymphatic system. BACH2 works closely with proteins like BCL6 and PRDM1 in these contexts influencing cell proliferation and survival pathways which can impact both tumor development and autoimmune pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator that acts as a repressor or activator (By similarity). Binds to Maf recognition elements (MARE) (By similarity). Plays an important role in coordinating transcription activation and repression by MAFK (By similarity). Induces apoptosis in response to oxidative stress through repression of the antiapoptotic factor HMOX1 (PubMed : 17018862). Positively regulates the nuclear import of actin (By similarity). Is a key regulator of adaptive immunity, crucial for the maintenance of regulatory T-cell function and B-cell maturation (PubMed : 28530713).
See full target information BACH2
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Product promise

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