Rabbit Polyclonal BACH2 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human BACH2 aa 750 to C-terminus.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human BACH2 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BYV9
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Transcriptional regulator that acts as repressor or activator (By similarity). Binds to Maf recognition elements (MARE) (By similarity). Plays an important role in coordinating transcription activation and repression by MAFK (By similarity). Induces apoptosis in response to oxidative stress through repression of the antiapoptotic factor HMOX1 (PubMed:17018862). Positively regulates the nuclear import of actin (By similarity). Is a key regulator of adaptive immunity, crucial for the maintenance of regulatory T-cell function and B-cell maturation (PubMed:28530713).
Alternative names
Transcription regulator protein BACH2, BTB and CNC homolog 2, BACH2
Recommended products
Rabbit Polyclonal BACH2 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human BACH2 aa 750 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human BACH2 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BYV9
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
BACH2 also known as BTB and CNC homology 2 is a transcription factor with a molecular mass of around 95 kDa. It regulates the expression of certain genes by binding to specific DNA sequences. BACH2 maintains a zinc-finger domain and a BTB domain which are important for its binding and transcriptional repressor functions. The target expresses predominantly in immune cells especially in B cells and T cells playing a critical role in immune system regulation. It is also found in other tissues but its levels are comparatively higher in lymphoid organs.
- Biological function summary
BACH2 plays an important role in the regulation of immune responses. It functions as a homodimer or forms heterodimers with other proteins such as BACH1 allowing it to modulate gene expression effectively. BACH2 regulates the balance between immune tolerance and activation by controlling the production of cytokines and the differentiation of T cells. This regulation is important for preventing autoimmune responses while enabling proper immune responses against infections.
- Pathways
BACH2 participates actively in the oxidative stress response and pathways of lymphocyte development. In the oxidative stress pathway it interacts with proteins like KEAP1 and NRF2 to regulate antioxidant gene expression. In lymphocyte development it influences the recombination of immunoglobulin genes and supports the maturation of B cells into plasma cells. By doing so BACH2 ensures the proper functioning of the immune system working in conjunction with various transcriptional regulatory networks.
- Associated diseases and disorders
BACH2 has associations with several immune-related conditions. It is implicated in autoimmune diseases such as type 1 diabetes where improper regulation may lead to immune system attacks on healthy cells. Additionally changes in BACH2 expression levels have been linked to lymphoma a type of blood cancer involving the lymphatic system. BACH2 works closely with proteins like BCL6 and PRDM1 in these contexts influencing cell proliferation and survival pathways which can impact both tumor development and autoimmune pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-BACH2 antibody (ab226394)
All lanes: Western blot - Anti-BACH2 antibody (ab226394) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 15 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 15 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Exposure time: 3min
Immunoprecipitation - Anti-BACH2 antibody (ab226394)
BACH2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN buffer; 1.0 mg for IP, 20% of IP loaded) with ab226394 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226394 at 1 μg/ml.
Lane 1: ab226394 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
All lanes: Immunoprecipitation - Anti-BACH2 antibody (ab226394)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Exposure time: 3min
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