AB62465

Anti-Bad antibody

Name: Anti-Bad antibody (ab62465) | Abcam
Brand: Abcam Limited
SKU: AB62465
Price: 515 USD
Availability: InStock

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(12 Publications)

Rabbit Polyclonal BAD antibody. Suitable for WB and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human BAD.

View Alternative Names

BBC6, BCL2L8, BAD, Bcl2-associated agonist of cell death, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-L-8

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Western blot - Anti-Bad antibody (AB62465)

Lane 1:

Western blot - Anti-Bad antibody (ab62465) at 0.5 µg/mL

Lane 2:

Western blot - Anti-Bad antibody (ab62465) at 1 µg/mL

Lane 3:

Western blot - Anti-Bad antibody (ab62465) at 2 µg/mL

All lanes:

T24 cell lysate at 15 µg

Secondary

All lanes:

Anti rabbit IgG secondary antibody

Predicted band size: 18 kDa

Observed band size: 22 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human BAD. The exact immunogen used to generate this antibody is proprietary information.

Q92934

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Bad protein also known as Bcl-2-associated death promoter plays a critical role in apoptosis regulation. It possesses a molecular weight of approximately 23 kDa. Bad is expressed widely in tissues and is a member of the Bcl-2 family of proteins. This family is well-known for its involvement in the regulation of cell death both promoting and inhibiting depending on the protein’s interactions.

Biological function summary

Its main role is to promote apoptosis by binding to anti-apoptotic proteins like Bcl-2 and Bcl-XL displacing pro-apoptotic Bax and Bak proteins to trigger cell death. Bad functions as part of the mitochondrial apoptosis pathway and is known to form complexes with phosphorylated proteins. Phosphorylation of Bad by kinases can lead to its sequestration to the cytoplasm reducing apoptosis.

Pathways

Its action is pivotal in the PI3K/Akt signaling pathway. This pathway involves growth factors and cell survival signals where phosphorylation of Bad inhibits its pro-apoptotic activity. Bad interacts with proteins like Akt and 14-3-3 to modulate cell survival. Disruption in its regulation through these pathways may lead to abnormal cell survival or death.

Bad has implications in cancer and neurodegenerative diseases. Cancer cells often exhibit impaired apoptotic pathways where overexpression of Bad can ameliorate responsiveness to therapy. In neurodegenerative diseases such as Alzheimer's dysregulation of its function can contribute to cell death. The protein’s interaction with Bcl-2 and Bcl-XL is significant in these contexts influencing disease propagation and severity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

See full target information BAD

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40565356

2025

Pro-Apoptotic Activity of 1-(4,5,6,7-Tetrabromo-1-benzimidazol-1-yl)propan-2-one, an Intracellular Inhibitor of PIM-1 Kinase in Acute Lymphoblastic Leukemia and Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Patrycja Wińska,Monika Wielechowska,Łukasz Milewski,Paweł Siedlecki,Edyta Łukowska-Chojnacka

Cancers 13: PubMed34572804

2021

The Stress-Inducible BCL2A1 Is Required for Ovarian Cancer Metastatic Progression in the Peritoneal Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Liang,Mingo M H Yung,Fangfang He,Peili Jiao,Karen K L Chan,Hextan Y S Ngan,David W Chan

The American journal of pathology 191:1537-1549 PubMed34139193

2021

Rictor/Mammalian Target of Rapamycin Complex 2 Signaling Protects Colonocytes from Apoptosis and Prevents Epithelial Barrier Breakdown.

Applications

Unspecified application

Species

Unspecified reactive species

Felipe Castro-Martinez,Aurora Candelario-Martinez,Maria R Encarnacion-Garcia,Zayda Piedra-Quintero,Raul Bonilla-Moreno,Abigail Betanzos,Rocio Perez-Orozco,Maria A Hernandez-Cueto,Jose E Muñoz-Medina,Genaro Patiño-Lopez,Michael Schnoor,Nicolas Villegas-Sepulveda,Porfirio Nava

Molecular medicine reports 23: PubMed33236129

2020

Chloroform extract of Markovich peel induces apoptosis and inhibits stemness in HeLa human cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Seul Choi,Jang Mi Han,Yue Jai Kang,Hye Jin Jung

Journal of cellular and molecular medicine : PubMed33135290

2020

circRNA 001306 enhances hepatocellular carcinoma growth by up-regulating CDK16 expression via sponging miR-584-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Liu,Cixiao Wang,Zeyou Jiang,Shan Li,Fang Li,Hua-Bing Tan,Su-Yang Yue

Pigment cell & melanoma research 33:542-555 PubMed31909885

2020

Extracellular vesicles released by melanocytes after UVA irradiation promote intercellular signaling via miR21.

Applications

Unspecified application

Species

Unspecified reactive species

Petra Wäster,Ida Eriksson,Linda Vainikka,Karin Öllinger

eLife 8: PubMed31294695

2019

The CUL5 ubiquitin ligase complex mediates resistance to CDK9 and MCL1 inhibitors in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shaheen Kabir,Justin Cidado,Courtney Andersen,Cortni Dick,Pei-Chun Lin,Therese Mitros,Hong Ma,Seung Hyun Baik,Matthew A Belmonte,Lisa Drew,Jacob E Corn

Journal of experimental & clinical cancer research 38:42 PubMed30691497

2019

Activation of dynamin-related protein 1 - dependent mitochondria fragmentation and suppression of osteosarcoma by cryptotanshinone.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Hau Yen,Hung Sen Huang,Chia Ju Chuang,Sheng-Teng Huang

Oncology letters 17:2498-2506 PubMed30675313

2019

Nonylphenol promotes the proliferation of colorectal cancer COLO205 cells by upregulating the expression of protein kinase C ζ.

Applications

Unspecified application

Species

Unspecified reactive species

Xuefeng Yang,Handong Huang,Maijian Wang,Xingbin Zheng,Ming Xie,Jie Xu

International journal of clinical and experimental 11:4562-4570 PubMed31949854

2018

The protective effect of the Rho-kinase inhibitor hydroxyfasudil on propofol-induced hippocampal neuron apoptosis in neonatal rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xuze Li,Lining Huang,Zhifang Zhao,Lijun Bo,Rongtian Kang,Jiaojiao Yang,Zhenming Dong

View all publications

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