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AB278558

Anti-Bad (phospho S112) antibody [BADS112-B9]

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(1 Publication)

Rabbit Recombinant Monoclonal BAD phospho S112 antibody. Suitable for Flow Cyt and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human BAD phospho S112.

View Alternative Names

BBC6, BCL2L8, BAD, Bcl2-associated agonist of cell death, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-L-8

4 Images
Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (AB278558)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (AB278558)

Peptide blocking flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or Calyculin A-treated (green) or CalA and blocked with phospho peptide (gold) or CalA and blocked with non-phospho peptide (purple) using ab278558 at 0.1 µg/mL.

Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (AB278558)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (AB278558)

Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with Calyculin A (green) using ab278558 at 0.01μg/mL.

Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (AB278558)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (AB278558)

Flow cytometric analysis of C6 cells secondary antibody only negative control (blue), or treated with imatinib (red) or with pervanadate (green) using ab278558 at 0.08μg/mL.

Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (AB278558)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (AB278558)

ab278558 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of NIH3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of ab278558 (green).

  • 578 PE

    PE Anti-Bad (phospho S112) antibody [BADS112-B9]

  • 660 APC

    APC Anti-Bad (phospho S112) antibody [BADS112-B9]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BADS112-B9

Isotype

IgG

Light chain type

lambda

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human BAD phospho S112. The exact immunogen used to generate this antibody is proprietary information.

Q92934

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Bad protein also known as Bcl-2-associated death promoter plays a critical role in apoptosis regulation. It possesses a molecular weight of approximately 23 kDa. Bad is expressed widely in tissues and is a member of the Bcl-2 family of proteins. This family is well-known for its involvement in the regulation of cell death both promoting and inhibiting depending on the protein’s interactions.
Biological function summary

Its main role is to promote apoptosis by binding to anti-apoptotic proteins like Bcl-2 and Bcl-XL displacing pro-apoptotic Bax and Bak proteins to trigger cell death. Bad functions as part of the mitochondrial apoptosis pathway and is known to form complexes with phosphorylated proteins. Phosphorylation of Bad by kinases can lead to its sequestration to the cytoplasm reducing apoptosis.

Pathways

Its action is pivotal in the PI3K/Akt signaling pathway. This pathway involves growth factors and cell survival signals where phosphorylation of Bad inhibits its pro-apoptotic activity. Bad interacts with proteins like Akt and 14-3-3 to modulate cell survival. Disruption in its regulation through these pathways may lead to abnormal cell survival or death.

Bad has implications in cancer and neurodegenerative diseases. Cancer cells often exhibit impaired apoptotic pathways where overexpression of Bad can ameliorate responsiveness to therapy. In neurodegenerative diseases such as Alzheimer's dysregulation of its function can contribute to cell death. The protein’s interaction with Bcl-2 and Bcl-XL is significant in these contexts influencing disease propagation and severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
See full target information BAD phospho S112
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 43:68 PubMed38439082

2024

MUC20 regulated by extrachromosomal circular DNA attenuates proteasome inhibitor resistance of multiple myeloma by modulating cuproptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobin Wang,Yingqing Shi,Hua Shi,Xiaoyu Liu,Aijun Liao,Zhuogang Liu,Robert Z Orlowski,Rui Zhang,Huihan Wang
View all publications
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Product promise

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