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Rabbit Recombinant Monoclonal BAD phospho S112 antibody. Carrier free. Suitable for IP, Dot, WB and reacts with Human, Rat, Synthetic peptide samples.

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Images

Immunoprecipitation - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (AB248343), expandable thumbnail
  • Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (AB248343), expandable thumbnail
  • Dot Blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (AB248343), expandable thumbnail
  • Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (AB248343), expandable thumbnail
  • Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (AB248343), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPDotWBIHC-PICC/IF
Human
Tested
Expected
Tested
Not recommended
Not recommended
Mouse
Predicted
Predicted
Predicted
Not recommended
Not recommended
Rat
Expected
Expected
Tested
Not recommended
Not recommended
Synthetic peptide
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Rat
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Human, Mouse, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Human, Mouse, Synthetic peptide
Dilution info
-
Notes

-

Target data

Function

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Alternative names

BBC6, BCL2L8, BAD, Bcl2-associated agonist of cell death, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-L-8

Recommended products

Rabbit Recombinant Monoclonal BAD phospho S112 antibody. Carrier free. Suitable for IP, Dot, WB and reacts with Human, Rat, Synthetic peptide samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR1891(2)
Purification technique
Affinity purification Protein A
Dissociation constant
4.7 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248343 is the carrier-free version of Anti-Bad (phospho S112) antibody [EPR1891(2)] ab129192.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Bad protein also known as Bcl-2-associated death promoter plays a critical role in apoptosis regulation. It possesses a molecular weight of approximately 23 kDa. Bad is expressed widely in tissues and is a member of the Bcl-2 family of proteins. This family is well-known for its involvement in the regulation of cell death both promoting and inhibiting depending on the protein’s interactions.

Biological function summary

Its main role is to promote apoptosis by binding to anti-apoptotic proteins like Bcl-2 and Bcl-XL displacing pro-apoptotic Bax and Bak proteins to trigger cell death. Bad functions as part of the mitochondrial apoptosis pathway and is known to form complexes with phosphorylated proteins. Phosphorylation of Bad by kinases can lead to its sequestration to the cytoplasm reducing apoptosis.

Pathways

Its action is pivotal in the PI3K/Akt signaling pathway. This pathway involves growth factors and cell survival signals where phosphorylation of Bad inhibits its pro-apoptotic activity. Bad interacts with proteins like Akt and 14-3-3 to modulate cell survival. Disruption in its regulation through these pathways may lead to abnormal cell survival or death.

Associated diseases and disorders

Bad has implications in cancer and neurodegenerative diseases. Cancer cells often exhibit impaired apoptotic pathways where overexpression of Bad can ameliorate responsiveness to therapy. In neurodegenerative diseases such as Alzheimer's dysregulation of its function can contribute to cell death. The protein’s interaction with Bcl-2 and Bcl-XL is significant in these contexts influencing disease propagation and severity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunoprecipitation - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343), expandable thumbnail

    Immunoprecipitation - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)

    This data was developed using Anti-Bad (phospho S112) antibody [EPR1891(2)] ab129192, the same antibody clone in a different buffer formulation.Anti-Bad (phospho S112) antibody [EPR1891(2)] ab129192 (purified) at 1/30 immunoprecipitating Bad in HeLa cell lysate treated with Calcyculin A (Lane 1). For western blotting a HRP-conjugated anti-rabbit IgG was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-Bad (phospho S112) antibody [EPR1891(2)] (Anti-Bad (phospho S112) antibody [EPR1891(2)] ab129192)

    Predicted band size: 18 kDa

  • Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343), expandable thumbnail

    Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)

    This data was developed using Anti-Bad (phospho S112) antibody [EPR1891(2)] ab129192, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] (Anti-Bad (phospho S112) antibody [EPR1891(2)] ab129192) at 1/5000 dilution

    Lane 1: Untreated C6 cells at 10 µg

    Lane 2: C6 cells treated with Calcyculin A at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 18 kDa

    Observed band size: 23 kDa

  • Dot Blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343), expandable thumbnail

    Dot Blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)

    This data was developed using Anti-Bad (phospho S112) antibody [EPR1891(2)] ab129192, the same antibody clone in a different buffer formulation.Dot blot analysis of Bad (phospho S112) using Anti-Bad (phospho S112) antibody [EPR1891(2)] ab129192 primary antibody at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as a secondary antibody at a dilution of 1/100,000.Lane 1: Bad (pS112) phospho peptide
    Lane 2: Bad non-phospho peptideBlocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343), expandable thumbnail

    Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)

    This data was developed using Anti-Bad (phospho S112) antibody [EPR1891(2)] ab129192, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] (Anti-Bad (phospho S112) antibody [EPR1891(2)] ab129192) at 1/20000 dilution

    Lane 1: Untreated HeLa cell lysate at 10 µg

    Lane 2: HeLa cell lysate treated with Calcyculin A at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 18 kDa

    Observed band size: 23 kDa

  • Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343), expandable thumbnail

    Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)

    This data was developed using Anti-Bad (phospho S112) antibody [EPR1891(2)] ab129192, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] (Anti-Bad (phospho S112) antibody [EPR1891(2)] ab129192) at 1/1000 dilution

    Lane 1: HeLa cell lysates, untreated at 10 µg

    Lane 2: HeLa cell lysates, treated with Calyculin A at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 18 kDa

  • OI-RD Scanning - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343), expandable thumbnail

    OI-RD Scanning - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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