JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB28824

Anti-Bad (phospho S99) antibody

4

(1 Review)

|

(16 Publications)

Rabbit Polyclonal BAD phospho S99 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human BAD phospho S99 aa 50-150.

View Alternative Names

BBC6, BCL2L8, BAD, Bcl2-associated agonist of cell death, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-L-8

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad (phospho S99) antibody (AB28824)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad (phospho S99) antibody (AB28824)

Immunohistochemical analysis of paraffin embedded breast carcinoma, using ab28824. Left : Untreated. Right : Treated with synthesized phosphopeptide.

Western blot - Anti-Bad (phospho S99) antibody (AB28824)
  • WB

Unknown

Western blot - Anti-Bad (phospho S99) antibody (AB28824)

All lanes:

Western blot - Anti-Bad (phospho S99) antibody (ab28824) at 1/500 dilution

Lane 1:

MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg

Lane 2:

U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 18 kDa

Observed band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human BAD phospho S99 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q92934

Specificity

Predicited to react with Mouse S136 and Rat S137

style
left-column

Alternative Products

Primary Antibodies

AB28825

Anti-Bad (phospho S155) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad (phospho S155) antibody (AB28825)

  • 3
  • 1
Primary Antibodies

AB62465

Anti-Bad antibody

Western blot - Anti-Bad antibody (AB62465)

  • 0
  • 0
Primary Antibodies

AB90435

Anti-Bad antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad antibody (AB90435)

  • 4
  • 1
Primary Antibodies

AB32445

Anti-Bad antibody [Y208]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad antibody [Y208] (AB32445)

  • 4
  • 13
Primary Antibodies

AB220116

Anti-Bad antibody [Y208] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad antibody [Y208] - BSA and Azide free (AB220116)

  • 0
  • 0
Primary Antibodies

AB216829

Anti-Bad (phospho S128) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad (phospho S128) antibody (AB216829)

  • 0
  • 0
Primary Antibodies

AB129192

Anti-Bad (phospho S112) antibody [EPR1891(2)]

RabMAb|Recombinant

Immunoprecipitation - Anti-Bad (phospho S112) antibody [EPR1891(2)] (AB129192)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32503

Anti-Bax antibody [E63]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (AB32503)

  • 4
  • 12
Primary Antibodies

AB32370

Anti-Bcl-XL antibody [E18]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Bcl-XL antibody [E18] (AB32370)

  • 5
  • 10
Primary Antibodies

AB32042

Anti-Cleaved Caspase-3 antibody [E83-77]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-Cleaved Caspase-3 antibody [E83-77] (AB32042)

  • 4
  • 28
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Bad protein also known as Bcl-2-associated death promoter plays a critical role in apoptosis regulation. It possesses a molecular weight of approximately 23 kDa. Bad is expressed widely in tissues and is a member of the Bcl-2 family of proteins. This family is well-known for its involvement in the regulation of cell death both promoting and inhibiting depending on the protein’s interactions.
Biological function summary

Its main role is to promote apoptosis by binding to anti-apoptotic proteins like Bcl-2 and Bcl-XL displacing pro-apoptotic Bax and Bak proteins to trigger cell death. Bad functions as part of the mitochondrial apoptosis pathway and is known to form complexes with phosphorylated proteins. Phosphorylation of Bad by kinases can lead to its sequestration to the cytoplasm reducing apoptosis.

Pathways

Its action is pivotal in the PI3K/Akt signaling pathway. This pathway involves growth factors and cell survival signals where phosphorylation of Bad inhibits its pro-apoptotic activity. Bad interacts with proteins like Akt and 14-3-3 to modulate cell survival. Disruption in its regulation through these pathways may lead to abnormal cell survival or death.

Bad has implications in cancer and neurodegenerative diseases. Cancer cells often exhibit impaired apoptotic pathways where overexpression of Bad can ameliorate responsiveness to therapy. In neurodegenerative diseases such as Alzheimer's dysregulation of its function can contribute to cell death. The protein’s interaction with Bcl-2 and Bcl-XL is significant in these contexts influencing disease propagation and severity.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
See full target information BAD phospho S99
style
left-column

Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:112595 PubMed37224013

2023

Endogenous Cyclin D1 Promotes the Rate of Onset and Magnitude of Mitogenic Signaling via Akt1 Ser473 Phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Chen,Xuanmao Jiao,Agnese Di Rocco,Duanwen Shen,Shaohua Xu,Adam Ertel,Zuoren Yu,Gabriele Di Sante,Min Wang,Zhiping Li,Timothy G Pestell,Mathew C Casimiro,Emmanuel Skordalakes,Samuel Achilefu,Richard G Pestell

Physiological reports 10:e15241 PubMed35388988

2022

FGF7 peptide (FGF7p) mimetic mitigates bladder urothelial injury from cyclophosphamide.

Applications

Unspecified application

Species

Unspecified reactive species

Sridhar Tatarao Narla,Lori Rice,David Ostrov,Steven G Swarts,Dietmar W Siemann,Daniel Scott Bushnell,Jacqueline G Holden,Joanne Lindsey Duara,Carlton Matthew Bates

The American journal of pathology 192:604-612 PubMed35063403

2022

AKT Signaling Downstream of KGF Is Necessary and Sufficient for Blocking Cyclophosphamide Bladder Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Sridhar T Narla,Daniel S Bushnell,Joanne L Duara,Carlton M Bates

Frontiers in pharmacology 12:713715 PubMed34381366

2021

The Dual Dose-Dependent Effects of Corticosterone on Hippocampal Cell Apoptosis After Traumatic Brain Injury Depend on the Activation Ratio of Mineralocorticoid Receptors to Glucocorticoid Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Zhang,Mengshi Yang,Qiongyu Yan,Xiaojian Xu,Fei Niu,Jinqian Dong,Yuan Zhuang,Shenghua Lu,Qianqian Ge,Baiyun Liu

Journal of neuroinflammation 17:318 PubMed33100225

2020

Corticosteroid receptor rebalancing alleviates critical illness-related corticosteroid insufficiency after traumatic brain injury by promoting paraventricular nuclear cell survival via Akt/CREB/BDNF signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Zhang,Miao Bai,Xiaojian Xu,Mengshi Yang,Fei Niu,Fei Gao,Baiyun Liu

Cell reports 32:108151 PubMed32937140

2020

Endogenous Cyclin D1 Promotes the Rate of Onset and Magnitude of Mitogenic Signaling via Akt1 Ser473 Phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Chen,Xuanmao Jiao,Agnese Di Rocco,Duanwen Shen,Shaohua Xu,Adam Ertel,Zuoren Yu,Gabriele Di Sante,Min Wang,Zhiping Li,Timothy G Pestell,Mathew C Casimiro,Emmanuel Skordalakes,Samuel Achilefu,Richard G Pestell

BioMed research international 2019:7298539 PubMed31772936

2019

Dihydrochalcone Derivative Induces Breast Cancer Cell Apoptosis via Intrinsic, Extrinsic, and ER Stress Pathways but Abolishes EGFR/MAPK Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wasitta Rachakhom,Patompong Khaw-On,Wilart Pompimon,Ratana Banjerdpongchai

PloS one 13:e0196805 PubMed29723246

2018

Early changes in rpS6 phosphorylation and BH3 profiling predict response to chemotherapy in AML cells.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Grundy,Thomas Jones,Liban Elmi,Michael Hall,Adam Graham,Nigel Russell,Monica Pallis

Cellular physiology and biochemistry : internation 41:451-465 PubMed28214890

2017

A New Perspective for Osteosarcoma Therapy: Proteasome Inhibition by MLN9708/2238 Successfully Induces Apoptosis and Cell Cycle Arrest and Attenuates the Invasion Ability of Osteosarcoma Cells in Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Renhao Liu,Chunjiang Fu,Jiabing Sun,Xvming Wang,Shuo Geng,Xiaoyu Wang,Jilong Zou,Zhenggang Bi,Chenglin Yang

Oncotarget 7:68314-68327 PubMed27582542

2016

Propranolol induced G0/G1/S phase arrest and apoptosis in melanoma cells via AKT/MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chengfang Zhou,Xiang Chen,Weiqi Zeng,Cong Peng,Gang Huang,Xian'an Li,Zhengxiao Ouyang,Yi Luo,Xuezheng Xu,Biaobo Xu,Weili Wang,Ruohui He,Xu Zhang,Liyang Zhang,Jie Liu,Todd C Knepper,Yijing He,Howard L McLeod
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com