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AB278338

Anti-Bad pSer75 antibody [EPR1891-88] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal BAD antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

BBC6, BCL2L8, BAD, Bcl2-associated agonist of cell death, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-L-8

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1891-88

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab278338 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Bad pSer75. The recommended pair for sandwich ELISA is:
Capture: ab278339, Human Bad pSer75 Capture Antibody (unconjugated)
Detector: ab278338, Human Bad pSer75 Detector Antibody (unconjugated)

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bad pSer75 also known as Bcl-2-associated death promoter Ser75 is a phosphorylation site of the BAD protein an important pro-apoptotic member of the Bcl-2 family. BAD is a protein that undergoes phosphorylation to regulate its function. Its mass is about 18 to 20 kDa and it expresses in various tissues including the brain heart and immune cells. The phosphorylation at serine 75 plays an important role in modulating BAD's interaction with other proteins.
Biological function summary

When phosphorylated at serine 75 BAD associates with 14-3-3 proteins which sequesters it in the cytoplasm and inhibits its pro-apoptotic activity. This modification is part of a larger signaling complex that influences cell survival. Phosphorylation of BAD affects its ability to bind to and neutralize anti-apoptotic proteins like Bcl-xL and Bcl-2 critical in balancing cell death and survival.

Pathways

BAD pSer75 phosphorylation integrates into survival pathways especially the PI3K/Akt and MAPK/ERK pathways. Akt and ERK kinases regulate this phosphorylation site and promote cell survival by inactivating BAD. These pathways interact with other players like mTOR and promote cellular responses to growth signals delineating a critical role in interpreting extracellular cues that affect apoptosis.

Dysregulation of BAD pSer75 phosphorylation connects to cancer and neurodegenerative disorders. In cancers abnormal phosphorylation could alter cell survival aiding the cancer cells’ resistance to apoptosis. BAD relates to proteins like Bcl-2 which also play roles in cancer pathways. In neurodegenerative diseases improper regulation of apoptosis contributes to cellular loss. Changes in BAD activity influenced through its phosphorylation contribute to the disease state by failing to maintain proper neuronal survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
See full target information BAD
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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